LOCUS Exported 3385 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5P. ACCESSION . VERSION . KEYWORDS p3E-ING2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3385) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3385) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3385 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(1..125) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(871..966) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1003..1022) /label=T7 /note="T7 promoter, forward primer" promoter complement(1004..1022) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1027..1043) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1027..1043) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1145..1164) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1156..1965 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1223..1242) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2112..2700 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2601..2620 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2854..2871 /label=L4440 /note="L4440 vector, forward primer" terminator 3030..3057 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3149..3235 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3284..3306 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3298..3315 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3299..3315 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc 61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa 121 gtggtggcaa gtccagtaga attcgcaatt gaccccaatg agccaactta ttgtctttgc 181 aatcaagtta gttatggcga gatgatagga tgcgacaacg agcagtgccc aatagagtgg 241 ttccatttca gttgcgtatc attgacatat aaaccaaagg gcaaatggta ttgcccaaag 301 tgcaggggtg acaatgagaa gacgatggac aagagtacag agaagacaaa aaaagagcga 361 cgtgctcgtg agttcgccag tccggtggag ttcgcgattg acccgaacga gcccacgtac 421 tgcttgtgta atcaggtatc ctacggggaa atgatcggat gcgacaatga acagtgtccc 481 atcgagtggt ttcacttttc ctgcgtgtct ttgacatata aacctaaagg aaaatggtac 541 tgcccaaagt gtagaggaga taatgaaaag actatggata agtctaccga gaagactaaa 601 aaggaacgcc gagcgaggga gttcgcaagt cccgttgaat ttgcgattga ccctaatgag 661 ccgacttact gcctttgcaa ccaggtttca tacggtgaga tgatcggctg tgacaacgaa 721 caatgcccaa tagaatggtt ccacttttca tgtgtgagtc ttacatataa acccaagggg 781 aaatggtatt gccccaagtg tcgaggcgac aatgaaaaaa cgatggacaa gagcacagaa 841 aaaaccaaaa aagagagacg tgcgcggtaa caactttatt atacaaagtt ggcattataa 901 aaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca 961 ttatttggag ctccatggta gcgttaacgc ggccgcgata tcccctatag tgagtcgtat 1021 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac 1081 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt 1141 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc 1201 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt 1261 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc 1321 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa 1381 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc 1441 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt 1501 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt 1561 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat 1621 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa 1681 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt 1741 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt 1801 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt 1861 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat 1921 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat 1981 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca 2041 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc 2101 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 2161 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 2221 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 2281 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 2341 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 2401 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 2461 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 2521 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 2581 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 2641 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 2701 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 2761 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 2821 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 2881 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 2941 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc 3001 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct 3061 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct 3121 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca 3181 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc 3241 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt 3301 aaaacgacgg ccagtcttaa gctcgggccc tgcagctcta gagctcgaat tctacaggtc 3361 actaatacca tctaagtagt tggtt //