LOCUS       Exported                4168 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Blasticidin-S resistance Vector for Phaeodactylum tricornutum.
ACCESSION   .
VERSION     .
KEYWORDS    pPTbsr
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Buck JM, Rio Bartulos C, Gruber A, Kroth PG
  TITLE     Blasticidin-S deaminase, a new selection marker for genetic 
            transformation of the diatom Phaeodactylum tricornutum.
  JOURNAL   PeerJ. 2018 Nov 14;6:e5884. doi: 10.7717/peerj.5884. eCollection 
            2018.
  PUBMED    30488015
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(1791..1810)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1792..1810)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1897..1914)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2068..2656)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2148..2167)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2827..3687)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3450..3469
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3688..3792)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3860..3878
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3916..3938)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4038..4057
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4138..4156
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4138..4155
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gggctgcagg acgcaatgga ggattatcac cgcaaaaatg aacttcgaaa aaaactttcg
       61 agcgaccatg gaaaaggagg atcagattca gattacaaca gtggattgct ctggtagcaa
      121 atatcttctg ctagattggc tcatggtcgg ttttggacgt tcgaagctca ccgtcaaaag
      181 aaacaaaaga gaagaatgac gtcttcgtga cgtagaatct acgactgtac tcggatctgg
      241 gaaatgaatt gactcacggt cttcttcgag tcctgttaca ggcccttggt ccgaaccccc
      301 acacgatttt tgcaccaaag atttgcttca atttgctgga tgttttgact gcaagatcag
      361 ctggcctagc aagagtgctc gtgttgcttc gtcgggaatc cctacgaatt tcagttctgc
      421 acaaatttgt ctgccgtttc gagaattcga tatcatcgac taattcgagc tcggtacccg
      481 gggatcctct agagtcgacc tgcaggcatg caagcttcag aagcgtgcta tcgaactcaa
      541 ccagggacgt gcggcacaaa tgggcatcct tgctctcatg gtgcacgaac agttgggagt
      601 ctctatcctt ccttaaaaat ttaattttca ttagttgcag tcactccgct ttggtttcac
      661 agtcaggaat aacactagct cgtcttcacc atggatgcca atctcgccta ttcatggtgt
      721 ataaaagttc aacatccaaa gctagaactt ttggaaagag aaagaatatc cgaatagggc
      781 acggcgtgcc gtattgttgg agtggactag cagaaagtga ggaaggcaca ggatgagttt
      841 tctcgagaca taccttcagc gtcgtcttca ctgtcacagt caactgacag taatcgttga
      901 tccggagaga ttcaaaattc aatctgtttg gacctggata agacacaaga gcgacatcct
      961 gacatgaacg ccgtaaacag caaatcctgg ttgaacacgt atccttttgg gggcctcccg
     1021 ctacgacgct cgctccagct ggggcttcct tactatacac agcgcgcata tttcacggtt
     1081 gccagatgtc aagacaattg gtttatgaaa acgtttaata tctcgcaaca agaccttgaa
     1141 cttgtcgaag tcgcaacgga aaaaattacg atgctttacg aagacaataa acatcatgtc
     1201 ggagcagcaa ttcgaacgaa aacgggagaa attatttcgg cagtccatat tgaagcatac
     1261 attggacgag tcacggtctg cgcagaagca attgcaattg gatcggcagt ctcgaatgga
     1321 caaaaagact ttgacacgat tgtcgcagtc cgacatccat actcggacga agtcgatcga
     1381 tcgattcgag tcgtctcgcc atgcggaatg tgccgagaac ttatttcgga ctacgcacca
     1441 gactgctttg tccttattga aatgaatgga aaacttgtca aaacgacgat tgaagaactt
     1501 attccactta aatacacgcg aaattgaaaa ccaggactga accttcctta aaaatttaat
     1561 tttcattagt tgcagtcact ccgctttggt ttcacagtca ggaataacac tagctcgtct
     1621 tcaccatgga tgccaatctc gcctattcat ggtgtataaa agttcaacat ccaaagctag
     1681 aacttttgga aagagaaaga atatccgaat agggcacggc gtgccgtatt gttggagtgg
     1741 actagcagaa agtgaggaag gcacaggatg agttttctcg aggccggtct ccctatagtg
     1801 agtcgtatta atttcgataa gccaggttaa cctgcattaa tgaatcggcc aacgcgcggg
     1861 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     1921 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1981 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     2041 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     2101 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     2161 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     2221 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     2281 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     2341 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     2401 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2461 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2521 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2581 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2641 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2701 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2761 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2821 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2881 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2941 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     3001 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     3061 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     3121 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     3181 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     3241 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     3301 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     3361 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     3421 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     3481 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     3541 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     3601 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     3661 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     3721 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3781 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     3841 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     3901 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     3961 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     4021 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat
     4081 attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
     4141 taggtgacac tatagaacca gatccccc
//
