LOCUS Exported 4906 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with Dynamin2a. ACCESSION . VERSION . KEYWORDS p3E-Dnm2a SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4906) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 4906) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4906 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(1..125) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2392..2487) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2524..2543) /label=T7 /note="T7 promoter, forward primer" promoter complement(2525..2543) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2548..2564) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2548..2564) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2666..2685) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2677..3486 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2744..2763) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3633..4221 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4122..4141 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 4375..4392 /label=L4440 /note="L4440 vector, forward primer" terminator 4551..4578 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 4670..4756 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 4805..4827 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 4819..4836 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 4820..4836 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc 61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa 121 gtggctggca accgggggat ggaggaattg atccccctga tcaataaact ccaagatgct 181 ttcagctcca ttggccaaag ttgtaacctg gatctgccgc agatagcagt tgtcggagga 241 cagagcgccg gaaaaagctc cgtgctggaa aatttcgtcg gcagggattt ccttccgcga 301 ggctcaggca tcgtcacccg caggcctctc attctgcagc tagtaaacaa caaagccgaa 361 tatgcagagt tcctacactg caaagggagg aagttcgtgg actttgatga agtccggcag 421 gagattgaag cagaaaccga caggattaca ggctccaata agggcatctc tcctatcccc 481 atcaacctga gagtctactc tcctaacgta ttgaatctga cactcatcga tttgcctggg 541 atgacgaagg tggcagtggg cgaccagccg cctgacatcg agcaccagat tcgggacatg 601 ataatgcagt ttatcaccag ggagagctgt ctaatccttg ccgtcacccc agccaacatg 661 gacttggcca attcggatgc cctcaaggtg gcgaaagaag tggaccctca aggactgcgt 721 accattggtg taatcactaa actggattta atggatgagg gtacagatgc ccgggatatc 781 ctggaaaaca aacttttacc acttcgtaga ggttatatcg gtgtagtgaa ccggagtcag 841 aaagacattg atggccgtaa ggacattcgt gctgcgctgg cagcagaaag gaagtttttc 901 ctgtcccacc ctagttacag acatatggct gaaaggatgg gaacgccaca cctgcaaaaa 961 gccctcaacc agcaattgac caaccacatc cgggacacac ttcccggctt gcggagcaaa 1021 ctccagtctc agcttctctc tctagaaaaa gaagtggaag agtataagaa cttccgccca 1081 gatgacccta ctcgaaagac caaggccctg ctgcagatgg tccagcaatt tggtgtggac 1141 tttgagaagt gcattgaggg atccggagat caggtggaca ctgcagagct gtctggaggt 1201 gccaggatta accgaatctt tcatgagcgc ttcccctttg agctggtcaa gatcgtgttt 1261 gatgaaaagg agctccggag agaaattagt cacgccatta agaacgtcca tggtgtcaga 1321 actgggctgt tcactccaga tctggcgttt gaagccatcg tgaaaaagca gatcattaag 1381 ctgaaagagc cctgtctgaa atgtatcgat ctggttattc aggagctcat caacacattc 1441 aggcagtgca ccaacaagtt aagctcgtac cctagactcc gagaagagac ggagaggatc 1501 gtcactactt acgtcagaga gagagaaggg aagaccaagg accaggttct tttgctgatt 1561 gacactgagc tgtcatacat caacaccaac cacgaggact tcattggatt tgcaaatgca 1621 cagcaaagga atatcgtcaa caagaagagg gccattccca accaggtgat ccgtaaaggc 1681 tggttaaccc tgaacatcag catcatgaag ggaggttcaa aggagtactg gttcgtcctg 1741 actgccgaat cgctgtcctg gtacaaagac gaggaggaaa aagagaagaa atacatgctt 1801 cctctggata acctgaagct cagagacgtg gagaaaggct tcatgtccac taagcacatt 1861 tttgcaatct tcaacactga gcaaaggaat gtgtataagg acttgcgtca gatcgagctg 1921 gcctgtgact cgcaggagga catggatagt tggaaagcct cttttctcag agcaggcgtt 1981 taccctgaga aagatcaagt ggagtcagaa gaggcagccc cagccgacag cttctccatg 2041 gaccctcagt tggaaagaca ggtcgagacc atccgtaacc tggtggactc ctatattgga 2101 atcgtcaaca aaaccatcag agacctcatg cccaagacta tcatgcatct catgatcaac 2161 agcgctaaag acttcatcca ctcagagctg ctggcgtacc tgtactcgtc tggagatcag 2221 aacagtctga tggaggagtc tgcagaccag gcccagcgca gagacgagat gctgaggatg 2281 taccatgcca tcaaagaggc actcagcatc atcggtgaca tcagcaccag caccatttcc 2341 acacctgtgc caccacccgt caacgacagc tggatacctg aaaccaggtg acaactttat 2401 tatacaaagt tggcattata aaaaagcatt gcttatcaat ttgttgcaac gaacaggtca 2461 ctatcagtca aaataaaatc attatttgga gctccatggt agcgttaacg cggccgcgat 2521 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg 2581 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa 2641 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg 2701 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc 2761 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca 2821 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc 2881 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact 2941 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta 3001 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg 3061 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct 3121 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt 3181 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg 3241 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa 3301 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc 3361 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa 3421 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt 3481 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg 3541 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg 3601 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 3661 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 3721 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 3781 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 3841 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 3901 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 3961 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 4021 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 4081 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 4141 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 4201 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 4261 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 4321 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 4381 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg 4441 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 4501 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat 4561 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc 4621 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac 4681 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag 4741 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg 4801 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc ctgcagctct 4861 agagctcgaa ttctacaggt cactaatacc atctaagtag ttggtt //