LOCUS Exported 5200 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with Dynamin2b. ACCESSION . VERSION . KEYWORDS p3E-Dnm2b SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5200) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 5200) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5200 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(1..125) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2686..2781) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2818..2837) /label=T7 /note="T7 promoter, forward primer" promoter complement(2819..2837) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2842..2858) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2842..2858) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2960..2979) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2971..3780 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(3038..3057) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3927..4515 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4416..4435 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 4669..4686 /label=L4440 /note="L4440 vector, forward primer" terminator 4845..4872 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 4964..5050 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 5099..5121 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 5113..5130 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 5114..5130 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc 61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa 121 gtggtgggga accgcgggat ggaggacttg attcccctca ttaacaagct gcatgacgcc 181 ttcagctcca tcggccagag ctgcaacctg gagctgccgc agatcgcggt ggtcggcgtg 241 cagagtgccg gcaagacctc cgtgctcgag aacttcgtgg gaagggactt tctgccgaga 301 ggaccaggaa tcgtcactcg caggcctctg attctccagc tggtgaacag taaagctgaa 361 tatgccgagt tcctgcactg taagggccgc aagtttgtgg actttgacga ggttcggatg 421 gagattgaag cagaaacaga ccgaatcact gaatctaaca ttggaatctc tgcagtgccc 481 attaacctgc gcctttactc gcccaacgtg ctgaatctga cgctgatcga tcttccgggc 541 ataacgaagg tggcggtggg tgaccagccg caggatatcg agtaccagat ccgggatatg 601 gtgttgcagt tcatctccaa agagaactgt ctgattctgg ccatcactcc agcaaacatg 661 gacctgacca actctgacgc tctcaagatc agcaaagagg tggaccctca tggtctgcgc 721 accatcggtg tgatcaccaa actggacctg atggatgaag gaaccgacgc cagagacatc 781 ctggagaaca agctgctgcc gctgcgcaga ggttatatcg gtgtggtgaa ccgcagtcag 841 aaggacattg atggcaggaa ggacatccgc gcggctcttt ctgctgagag gaagttcttc 901 ctgtcccatc cagcatacag acacatagca gagagaatgg gcacaccgta cctgcagaaa 961 acactcaacc agcaactgat gaaccacatc cgcgacacac tgccggcgct gcgcagtaaa 1021 ctgcagtctc agctgctgtc gctggagaaa gaggtggagg agtacaagaa cttcaaacca 1081 gacgatcctg cgcgcaaaac caaagccctg ctgcagatgg tccagcagtt cggtgtggac 1141 tttgagaagc gtatcggggg ctctggagat caggtggaca ctctggaact ctccggcgga 1201 gctcgaataa accgcatctt ccacgagcgc ttccccttcg agctggtgaa gacggagttt 1261 gatgagaagg agttgcggag ggagatcagt tacgccatca agaacattca cggagtcagg 1321 acggggctct tcaccccaga tctggccttc gaggccatcg tgaaaaagca gctgataaag 1381 ctgaaggagc cgtgcctcaa gtgcatcgac ctggtcatcc aggagctgat caacacggtc 1441 aggcagagca cactgaagct gaagttgtat cctcggctga gagaagagac ggagagaatc 1501 atcacaatat atgtcagaga gagagagacc aaaaccaaag accagatgat gctgctgatc 1561 gatgtccagc tctcctacat caacacaaac cacgaggact tcatcggatt cgccaatgcg 1621 caacagagca gcagagatgt cactaagaag caagccgttc ccaatcaggt gatccgccgt 1681 ggttggctga ccatcaacat cagcatcatg aagggcggct ccaaagacta ctggttcgtg 1741 ctgacggcgg agtctctgtc ctggtacaag gatgaggagg agaaggagaa gaagttcatg 1801 ctgccgctgg ataacctgaa gctgcgggat gtggagaaga gcttcatgag caacaaacac 1861 accttcggca tcttcaacac cgagcagagg aacgtgtata aagacctgcg tcagatcgag 1921 ctggcctgcg ccactcagga ggatgtggac agctggaagg cctctttcct cagagcggga 1981 gtctatcctg agaaagacca ggtggagaac ggggacgtgt cagcagactt tctctccatg 2041 gaccctcagc tggagcgtca ggttgagacc atcagtaatc tggtggactc gtacatcagc 2101 atcgtcaaca agagcatccg agacctgatg cccaaaacca tcatgcacct gatgatcaac 2161 agcacgaagg agttcatcta ctcggagctg ctggcgtacc tgtactcgtc aggtgatcag 2221 aacagtctga tggaggagtc ggcggagcag gagcagcggc gggacgagat gctccgcatg 2281 tacaacgctc tgaaagaggc tctgaagatc atcggggacg tcagcacatc caccttcacc 2341 acacctgtgc ccgcgccggt ggacgacacc tggatacaca ctgaccccag tcctccgtct 2401 cagcgtaagc cagctccgcc cactgtccgc cctcctgcag tgcgaggctc cacccccggt 2461 gctccagccc caccttctct aaacccctcc cctgccttcg gagctccgcc tgttccctcc 2521 agacccacca cattggagcc agctgtagct ccgccccctt gtgttccatc tcgccctgca 2581 caggtcccgc ccactgtgct gcccgggata cgcagtagga ggcctccagc ggctccaaac 2641 aggcccacaa tcatccgtcc gacagaacca tctctgctgg actgacaact ttattataca 2701 aagttggcat tataaaaaag cattgcttat caatttgttg caacgaacag gtcactatca 2761 gtcaaaataa aatcattatt tggagctcca tggtagcgtt aacgcggccg cgatatcccc 2821 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa 2881 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct 2941 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg 3001 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat 3061 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg 3121 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta 3181 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat 3241 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa 3301 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat 3361 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg 3421 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc 3481 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca 3541 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata 3601 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta 3661 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt 3721 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa 3781 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc 3841 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac 3901 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 3961 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 4021 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 4081 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 4141 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 4201 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 4261 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 4321 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 4381 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 4441 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 4501 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 4561 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 4621 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 4681 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 4741 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 4801 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca 4861 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc 4921 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga 4981 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc 5041 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc 5101 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccctgcag ctctagagct 5161 cgaattctac aggtcactaa taccatctaa gtagttggtt //