LOCUS Exported 3220 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with RhoAa. ACCESSION . VERSION . KEYWORDS p3E-RhoAa SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3220) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3220) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3220 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(1..125) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(706..801) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(838..857) /label=T7 /note="T7 promoter, forward primer" promoter complement(839..857) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(862..878) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(862..878) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(980..999) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 991..1800 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1058..1077) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 1947..2535 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2436..2455 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2689..2706 /label=L4440 /note="L4440 vector, forward primer" terminator 2865..2892 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 2984..3070 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3119..3141 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3133..3150 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3134..3150 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc 61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa 121 gtggtggctg cgattcgtaa gaagcttgta atcgttggag atggagcctg tggaaagacg 181 tgtttactca tcgttttcag taaagaccag tttcctgagg tttacgttcc cacagtgttt 241 gagaactatg tcgctgatat tgaggtggat tcaaaacagg tggaactggc tctatgggat 301 acagctggcc aagaggacta cgacaggctg aggcccctgt cctacccaga cactgatgtc 361 atccttatgt gcttttccat agacagtcct gacagtttgg agaacatccc agagaaatgg 421 acgccagaag taaaacattt ctgccctaat gttccaataa tactggtggg taataaaaaa 481 gatttacgga atgacgagca cactcgacgg gagctgcaga agatgaaaca ggaaccagtt 541 aaaccagagg agggacgaga catggctaat cgcatcaatg ccttcggcta ccttgagtgt 601 tcagctaaaa ctaaggaggg cgtgagggaa gtgtttgaaa tggccacaag agcggcgctc 661 caagccaaga aacgtggcaa gaagaatgcc tgcgctttgc tatagcaact ttattataca 721 aagttggcat tataaaaaag cattgcttat caatttgttg caacgaacag gtcactatca 781 gtcaaaataa aatcattatt tggagctcca tggtagcgtt aacgcggccg cgatatcccc 841 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa 901 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct 961 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg 1021 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat 1081 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg 1141 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta 1201 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat 1261 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa 1321 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat 1381 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg 1441 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc 1501 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca 1561 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata 1621 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta 1681 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt 1741 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa 1801 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc 1861 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac 1921 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1981 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 2041 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 2101 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 2161 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 2221 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 2281 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 2341 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 2401 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 2461 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 2521 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 2581 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 2641 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 2701 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 2761 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 2821 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca 2881 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc 2941 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga 3001 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc 3061 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc 3121 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccctgcag ctctagagct 3181 cgaattctac aggtcactaa taccatctaa gtagttggtt //