LOCUS       Exported                3508 bp ds-DNA     linear   SYN 10-MAR-2022
DEFINITION  To generate Adeno-Associated viruses for expression of dLight1.1 
            under CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CAG-dLight1.1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Patriarchi T, Cho JR, Merten K, Howe MW, Marley A, Xiong WH, Folk 
            RW, Broussard GJ, Liang R, Jang MJ, Zhong H, Dombeck D, von Zastrow 
            M, Nimmerjahn A, Gradinaru V, Williams JT, Tian L
  TITLE     Ultrafast neuronal imaging of dopamine dynamics with designed 
            genetically encoded sensors.
  JOURNAL   Science. 2018 Jun 29;360(6396). pii: science.aat4422. doi: 
            10.1126/science.aat4422. Epub 2018 May 31.
  PUBMED    29853555
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..278
                     /label=chicken beta-actin promoter
     primer_bind     557..579
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     641..660
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1663..1684
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1790..1811)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2051..2070)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2756..3344
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2809..2829)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3227..3238)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3387..3508
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3398..3417)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3452..3471
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      121 ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg
      181 cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc
      241 ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc
      301 gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
      361 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
      421 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg
      481 gccctttgtg cggggggagc ggctcggggc tgtccgcggg gggacggctg ccttcggggg
      541 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
      601 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
      661 ctgtctcatc attttggcaa agaattggat cctgcgcgcc atgaagacga tcatcgccct
      721 gagctacatc ttctgcctgg tgttcgccga ctacaaggac gatgatgacg ccatgaggac
      781 tctgaacacc tctgccatgg acgggactgg gctggtggtg gagagggact tctctgttcg
      841 tatcctcact gcctgtttcc tgtcgctgct catcctgtcc acgctcctgg ggaacacgct
      901 ggtctgtgct gccgttatca ggttccgaca cctgcggtcc aaggtgacca acttctttgt
      961 catctccttg gctgtgtcag atctcttggt ggccgtcctg gtcatgccct ggaaggcagt
     1021 ggctgagatt gctggcttct ggccctttgg gtccttctgt aacatctggg tggcctttga
     1081 catcatgtgc tccactgcat ccatcctcaa cctctgtgtg atcagcgtgg acaggtattg
     1141 ggctatctcc agccctttcc ggtatgagag aaagatgacc cccaaggcag ccttcatcct
     1201 gatcagtgtg gcatggacct tgtctgtact catctccttc atcccagtgc agctcagctg
     1261 gcacaaggca aaacccacaa gcccctctga tggaaatgcc acttccctgg ctgagaccat
     1321 agacaactgt gactccagcc tcagcaggac atatgccatc tcatcctctg taatcagctt
     1381 ttacatccct gtggccatca tgattgtcac ctacaccagg atctacagga ttgctcagaa
     1441 actgagctca ctcattaacg tctatatcaa ggccgacaag cagaagaacg gcatcaaggc
     1501 gaacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgcct accactacca
     1561 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcgt
     1621 gcagtccaaa ctttcgaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     1681 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggcg gtaccggagg
     1741 gagcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     1801 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggtgagg gcgatgccac
     1861 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     1921 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     1981 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacatccagg agcgcaccat
     2041 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2101 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2161 gcacaagctg gagtacaaca atcatgacca actgaaaaga gaaactaaag tcctgaagac
     2221 tctgtcggtg atcatgggtg tgtttgtgtg ctgttggcta cctttcttca tcttgaactg
     2281 cattttgccc ttctgtgggt ctggggagac gcagcccttc tgcattgatt ccaacacctt
     2341 tgacgtgttt gtgtggtttg ggtgggctaa ttcatccttg aaccccatca tttatgcctt
     2401 taatgctgat tttcggaagg cattttcaac cctcttagga tgctacagac tttgccctgc
     2461 gacgaataat gccatagaga cggtgagtat caataacaat ggggccgcga tgttttccag
     2521 ccatcatgag ccacgaggct ccatctccaa ggagtgcaat ctggtttacc tgatcccaca
     2581 tgctgtgggc tcctctgagg acctgaaaaa ggaggaggca gctggcatcg ccagaccctt
     2641 ggagaagctg tccccagccc tatcggtcat attggactat gacactgacg tctctctgga
     2701 gaagatccaa cccatcacac aaaacggtca gcacccaacc tgaaagctta tcgataatca
     2761 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     2821 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     2881 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     2941 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     3001 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     3061 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3121 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     3181 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     3241 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     3301 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     3361 cgggcggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     3421 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     3481 aaccattata agctgcaata aacaagtt
//
