LOCUS Exported 6427 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pbabe-c-mycT58A+HRasG12V SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6427) AUTHORS Kendall SD, Linardic CM, Adam SJ, Counter CM TITLE A network of genetic events sufficient to convert normal human cells to a tumorigenic state. JOURNAL Cancer Res. 2005 Nov 1. 65(21):9824-8. PUBMED 16267004 REFERENCE 2 (bases 1 to 6427) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6427 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 44..513 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 578..777 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 978..1394 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1324..1346 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1362..1378 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" primer_bind complement(2870..2890) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 2875..3204 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3055..3190 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3117..3136 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3217..3786 /codon_start=1 /product="human oncoprotein generated by the G12V mutation in the small GTPase H-Ras" /label=H-Ras (G12V) /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI RQHKLRKLNPPDESGPGCMSCKCVLS" LTR 3957..4426 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(4556..4573) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4727..5315) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4807..4826) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5475..6335) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6098..6117 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6336..13) /gene="bla" /label=AmpR promoter ORIGIN 1 taggggttcc gcgcacattt ccccgaaaag tgccacctgc agcctgaata tgggccaaac 61 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct 121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 181 acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt 241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc 301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc 361 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata 421 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg 481 agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga 541 cccctgccca gggaccaccg acccaccacc gggaggcaag ctggccagca acttatctgt 601 gtctgtccga ttgtctagtg tctatgactg attttatgcg cctgcgtcgg tactagttag 661 ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttctga acacccggcc 721 gcaaccctgg gagacgtccc agggactttg ggggccgttt ttgtggcccg acctgaggaa 781 gggagtcgat gtggaatccg accccgtcag gatatgtggt tctggtagga gacgagaacc 841 taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttggaaccga agccgcgcgt 901 cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt 961 atttgtctga aaattagggc cagactgtta ccactccctt aagtttgacc ttaggtcact 1021 ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag agacgttggg 1081 ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga gacggcacct 1141 ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc ccgcatggac 1201 acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac ccccctccct 1261 gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc 1321 tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc 1381 cttctctagg cgccggccgg atcccagtgt ggtggtacgc ttgcatgcct gcaggtcgag 1441 ctgtttgaag gctggatttc ctttgggcgt tggaaacccc gcagacagcc acgacgatgc 1501 ccctcaacgt gaacttcacc aacaggaact atgacctcga ctacgactcc gtacagccct 1561 atttcatctg cgacgaggaa gagaatttct atcaccagca acagcagagc gagctgcagc 1621 cgcccgcgcc cagtgaggat atctggaaga aattcgagct gcttcccgcc ccgcccctgt 1681 ccccgagccg ccgctccggg ctctgctctc catcctatgt tgcggtcgct acgtccttct 1741 ccccaaggga agacgatgac ggcggcggtg gcaacttctc caccgccgat cagctggaga 1801 tgatgaccga gttacttgga ggagacatgg tgaaccagag cttcatctgc gatcctgacg 1861 acgagacctt catcaagaac atcatcatcc aggactgtat gtggagcggt ttctcagccg 1921 ctgccaagct ggtctcggag aagctggcct cctaccaggc tacgcgcaaa gacagcacca 1981 gcctgagccc cgcccgcggg cacagcgtct gctccacctc cagcctgtac ctgcaggacc 2041 tcaccgccgc cgcgtccgag tgcattgacc cctcagtggt ctttccctac ccgctcaacg 2101 acagcagctc gcccaaatcc tgtacctcgt ccgattccac ggccttctct ccttcctcgg 2161 actcgctgct gtcctccgag tcctccccac gggccagccc tgagccccta gtgctgcatg 2221 aggagacacc gcccaccacc agcagcgact ctgaagaaga gcaagaagat gaggaagaaa 2281 ttgatgtggt gtctgtggag aagaggcaaa cccctgccaa gaggtcggag tcgggctcat 2341 ctccatcccg aggccacagc aaacctccgc acagcccact ggtcctcaag aggtgccacg 2401 tctccactca ccagcacaac tacgccgcac ccccctccac aaggaaggac tatccagctg 2461 ccaagagggc caagttggac agtggcaggg tcctgaagca gatcagcaac aaccgcaagt 2521 gctccagccc caggtcctca gacacggagg aaaacgacaa gaggcggaca cacaacgtct 2581 tggaacgtca gaggaggaac gagctgaagc gcagcttttt tgccctgcgt gaccagatcc 2641 ctgaattgga aaacaacgaa aaggccccca aggtagtgat cctcaaaaaa gccaccgcct 2701 acatcctgtc cattcaagca gacgagcaca agctcacctc tgaaaaggac ttattgagga 2761 aacgacgaga acagttgaaa cacaaactcg aacagcttcg aaactctcgt gcataaactg 2821 acctaactcg actctagtag gaattcgcca gcacagtggt cgaccctgtg gaatgtgtgt 2881 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 2941 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 3001 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 3061 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 3121 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 3181 tttggaggcc taggcttttg caaaaagctt gccaccatga cggaatataa gctggtggtg 3241 gtgggcgccg tcggtgtggg caagagtgcg ctgaccatcc agctgatcca gaaccatttt 3301 gtggacgaat acgaccccac tatagaggat tcctaccgga agcaggtggt cattgatggg 3361 gagacgtgcc tgttggacat cctggatacc gccggccagg aggagtacag cgccatgcgg 3421 gaccagtaca tgcgcaccgg ggagggcttc ctgtgtgtgt ttgccatcaa caacaccaag 3481 tcttttgagg acatccacca gtacagggag cagatcaaac gggtgaagga ctcggatgac 3541 gtgcccatgg tgctggtggg gaacaagtgt gacctggctg cacgcactgt ggaatctcgg 3601 caggctcagg acctcgcccg aagctacggc atcccctaca tcgagacctc ggccaagacc 3661 cggcagggag tggaggatgc cttctacacg ttggtgcgtg agatccggca gcacaagctg 3721 cggaagctga accctcctga tgagagtggc cccggctgca tgagctgcaa gtgtgtgctc 3781 tcctgataaa tcgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa 3841 agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa 3901 aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga 3961 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac 4021 agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 4081 ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa 4141 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact 4201 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa 4261 gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc 4321 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg 4381 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacatg cagcatgtat 4441 caaaattaat ttggtttttt ttcttaagta tttacattaa atggccatag ttgcattaat 4501 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 4561 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 4621 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 4681 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 4741 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 4801 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 4861 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 4921 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 4981 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 5041 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 5101 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 5161 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 5221 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 5281 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 5341 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 5401 aaaggatctt cacctagatc cttttgcggc cgcaaatcaa tctaaagtat atatgagtaa 5461 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 5521 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 5581 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 5641 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 5701 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 5761 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 5821 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 5881 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 5941 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 6001 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 6061 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 6121 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 6181 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 6241 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 6301 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 6361 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 6421 aaacaaa //