LOCUS       Exported               10792 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Fluorescent reporter of membrane voltage with improved sensitivity.
ACCESSION   .
VERSION     .
KEYWORDS    DRH334: FCK-QuasAr2-mO2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10792)
  AUTHORS   Hochbaum DR, Zhao Y, Farhi SL, Klapoetke N, Werley CA, Kapoor V, Zou
            P, Kralj JM, Maclaurin D, Smedemark-Margulies N, Saulnier JL, 
            Boulting GL, Straub C, Cho YK, Melkonian M, Wong GK, Harrison DJ, 
            Murthy VN, Sabatini BL, Boyden ES, Campbell RE, Cohen AE
  TITLE     All-optical electrophysiology in mammalian neurons using engineered 
            microbial rhodopsins.
  JOURNAL   Nat Methods. 2014 Aug;11(8):825-33. doi: 10.1038/nmeth.3000. Epub 
            2014 Jun 22.
  PUBMED    24952910
REFERENCE   2  (bases 1 to 10792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10792
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(42..61)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        233..612
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        614..812
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     767..787
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             830..1010
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1057..1182
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1675..1908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2093..2137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2435..2552
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2597..3885
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             3898..4656
                     /codon_start=1
                     /product="achaerhodopsin-3 (Arch) derivative with improved 
                     voltage sensitivity (Hochbaum et al., 2014)"
                     /label=QuasAr2
                     /translation="MVSIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILVSGIASAAYLSMFFGIGLTEVSVGGEMLDIYYARYAQWLFTTPLLL
                     LHLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYVLA
                     TSLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGAD"
     CDS             4663..5370
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEYNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    5479..6067
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5532..5552)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5950..5961)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6070..6086)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6071..6087)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6592..6772
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6798..6815)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6804..7028
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7074..7502
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7161..7180)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7371..7392
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7511..7531)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7516..7845
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7696..7831
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7758..7777
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7893..7940
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7959..8333
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8463..8584
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8500..8519)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8554..8573
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8633..8649)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8633..8649)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8646..8668)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8657..8673
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8681..8711)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8726..8747
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8864..8881)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9035..9623)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9115..9134)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9794..10654)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10417..10436
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10655..10759)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
       61 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
      121 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
      181 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
      241 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      301 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      361 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      421 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      481 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      541 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      601 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      661 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      721 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      781 aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg
      841 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      901 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      961 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     1021 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1861 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1981 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2281 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     2401 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2461 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2521 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2581 cagtttggtt aattaacatt atggccttag gtcacttcat ctccatgggg ttcttcttct
     2641 gattttctag aaaatgagat gggggtgcag agagcttcct cagtgacctg cccagggtca
     2701 catcagaaat gtcagagcta gaacttgaac tcagattact aatcttaaat tccatgcctt
     2761 gggggcatgc aagtacgata tacagaagga gtgaactcat tagggcagat gaccaatgag
     2821 tttaggaaag aagagtccag ggcagggtac atctacacca cccgcccagc cctgggtgag
     2881 tccagccacg ttcacctcat tatagttgcc tctctccagt cctaccttga cgggaagcac
     2941 aagcagaaac tgggacagga gccccaggag accaaatctt catggtccct ctgggaggat
     3001 gggtggggag agctgtggca gaggcctcag gaggggccct gctgctcagt ggtgacagat
     3061 aggggtgaga aagcagacag agtcattccg tcagcattct gggtctgttt ggtacttctt
     3121 ctcacgctaa ggtggcggtg tgatatgcac aatggctaaa aagcagggag agctggaaag
     3181 aaacaaggac agagacagag gccaagtcaa ccagaccaat tcccagagga agcaaagaaa
     3241 ccattacaga gactacaagg gggaagggaa ggagagatga attagcttcc cctgtaaacc
     3301 ttagaaccca gctgttgcca gggcaacggg gcaatacctg tctcttcaga ggagatgaag
     3361 ttgccagggt aactacatcc tgtctttctc aaggaccatc ccagaatgtg gcacccacta
     3421 gccgttacca tagcaactgc ctctttgccc cacttaatcc catcccgtct gttaaaaggg
     3481 ccctatagtt ggaggtgggg gaggtaggaa gagcgatgat cacttgtgga ctaagtttgt
     3541 tcgcatcccc ttctccaacc ccctcagtac atcaccctgg gggaacaggg tccacttgct
     3601 cctgggccca cacagtcctg cagtattgtg tatataaggc cagggcaaag aggagcaggt
     3661 tttaaagtga aaggcaggca ggtgttgggg aggcagttac cggggcaacg ggaacagggc
     3721 gtttcggagg tggttgccat ggggacctgg atgctgacga aggctcgcga ggctgtgagc
     3781 agccacagtg ccctgctcag aagccccaag ctcgtcagtc aagccggttc tccgtttgca
     3841 ctcaggagca cgggcaggcg agtggcccct agttctgggg gcagcggggg atccaccatg
     3901 gtaagtatcg ctctgcaggc tggttacgac ctactgggtg acggcagacc tgaaaccctg
     3961 tggctgggca tcggcactct gctgatgctg attggaacct tctactttct ggtccgcgga
     4021 tggggagtca ccgataagga tgcccgggaa tattacgctg tgactatcct ggtgtccgga
     4081 atcgcatccg ccgcatatct gtctatgttc tttggtatcg ggcttactga ggtgtccgtc
     4141 gggggcgaaa tgttggatat ctattatgcc aggtacgccc agtggctgtt taccacccca
     4201 cttctgctgc tgcacctggc ccttctcgct aaggtggatc gggtgaccat cggcaccctg
     4261 gtgggtgtgg acgccctgat gatcgtcact ggcctcatcg gagccttgag ccacacggcc
     4321 atagccagat acagttggtg gttgttctct acaatttgca tgatagtggt gctctatgtt
     4381 ctggctacat ccctgcgatc tgctgcaaag gagcggggcc ccgaggtggc atctaccttt
     4441 aacaccctga cagctctggt cttggtgctg tggaccgctt accctatcct gtggatcata
     4501 ggcactgagg gcgctggcgt ggtgggcctg ggcatcgaaa ctctgctgtt tatggtgttg
     4561 gacgtgactg ccaaggtcgg ctttggcttt atcctgttga gatcccgggc tattctgggc
     4621 gacaccgagg caccagaacc cagtgccggt gccgatacgt tgatggtgag caagggcgag
     4681 gagtataaca tggccatcat caaggagttc atgcgcttca aggtgcgcat ggagggctcc
     4741 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcttt
     4801 cagaccgcta agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     4861 tcccctcatt tcacctacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     4921 tacttcaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa ctacgaggac
     4981 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     5041 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg tgatgcagaa gaagaccatg
     5101 ggctgggagg cctcctccga gcggatgtac cccgaggacg gtgccctgaa gggcaagatc
     5161 aagatgaggc tgaagctgaa ggacggcggc cactacacct ccgaggtcaa gaccacctac
     5221 aaggccaaga agcccgtgca gctgcccggc gcctacatcg tcgacatcaa gttggacatc
     5281 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     5341 tccaccggcg gcatggacga gctgtacaag agtagaatca caagcgaagg cgagtacatc
     5401 cccctggatc aaatagacat aaatgtaggt ggattttgtt atgagaatga ataagaattc
     5461 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     5521 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     5581 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     5641 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     5701 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     5761 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     5821 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     5881 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     5941 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6001 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6061 tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc
     6121 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     6181 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     6241 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     6301 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     6361 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     6421 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     6481 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     6541 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     6601 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6661 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6721 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
     6781 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     6841 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     6901 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     6961 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     7021 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     7081 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     7141 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     7201 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     7261 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     7321 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     7381 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     7441 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     7501 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     7561 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     7621 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     7681 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7741 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     7801 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     7861 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
     7921 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
     7981 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
     8041 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
     8101 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
     8161 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
     8221 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
     8281 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
     8341 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     8401 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     8461 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8521 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8581 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     8641 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8701 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8761 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8821 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     8881 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8941 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9001 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9061 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9121 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9181 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9241 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9301 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9361 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9421 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     9481 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9541 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9601 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9661 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9721 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9781 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9841 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9901 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9961 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    10021 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    10081 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    10141 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10201 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    10261 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    10321 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    10381 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    10441 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10501 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10561 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10621 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10681 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10741 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc ga
//
