LOCUS       Exported                7623 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    HA-HIF1alpha-wt-pBabe-puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Yan Q, Bartz S, Mao M, Li L, Kaelin WG
  TITLE     The hypoxia-inducible factor 2alpha N-terminal and C-terminal 
            transactivation domains cooperate to promote renal tumorigenesis in 
            vivo.
  JOURNAL   Mol Cell Biol. 2007 Mar . 27(6):2092-102.
  PUBMED    17220275
REFERENCE   2  (bases 1 to 7623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    530..729
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             930..1346
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1276..1298
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1314..1330
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1378..1404
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      1417..1426
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(3941..3961)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3946..4275
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4126..4261
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4188..4207
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4285..4884
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4285..4304)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4781..4801
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5105..5574
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5704..5721)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5875..6463)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5955..5974)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6623..7483)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7246..7265
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7484..7588)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             7619..465
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
       61 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
      121 tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac
      181 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
      241 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
      301 agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc
      361 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
      421 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc
      481 gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggca agctggccag
      541 caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg cgcctgcgtc
      601 ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttct
      661 gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt ttttgtggcc
      721 cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg gttctggtag
      781 gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttggaacc
      841 gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt ctctgtctga
      901 ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc ttaagtttga
      961 ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga
     1021 agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc
     1081 gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg
     1141 gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg
     1201 acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat
     1261 ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc
     1321 cagccctcac tccttctcta ggcgccggcc ggatcgaaga ctaagcttac catggcctac
     1381 ccctacgacg tgcccgacta cgcctccctc ggatccgcca ccatggaggg cgccggcggc
     1441 gcgaacgaca agaaaaagat aagttctgaa cgtcgaaaag aaaagtctcg agatgcagcc
     1501 agatctcggc gaagtaaaga atctgaagtt ttttatgagc ttgctcatca gttgccactt
     1561 ccacataatg tgagttcgca tcttgataag gcctctgtga tgaggcttac catcagctat
     1621 ttgcgtgtga ggaaacttct ggatgctggt gatttggata ttgaagatga catgaaagca
     1681 cagatgaatt gcttttattt gaaagccttg gatggttttg ttatggttct cacagatgat
     1741 ggtgacatga tttacatttc tgataatgtg aacaaataca tgggattaac tcagtttgaa
     1801 ctaactggac acagtgtgtt tgattttact catccatgtg accatgagga aatgagagaa
     1861 atgcttacac acagaaatgg ccttgtgaaa aagggtaaag aacaaaacac acagcgaagc
     1921 ttttttctca gaatgaagtg taccctaact agccgaggaa gaactatgaa cataaagtct
     1981 gcaacatgga aggtattgca ctgcacaggc cacattcacg tatatgatac caacagtaac
     2041 caacctcagt gtgggtataa gaaaccacct atgacctgct tggtgctgat ttgtgaaccc
     2101 attcctcacc catcaaatat tgaaattcct ttagatagca agactttcct cagtcgacac
     2161 agcctggata tgaaattttc ttattgtgat gaaagaatta ccgaattgat gggatatgag
     2221 ccagaagaac ttttaggccg ctcaatttat gaatattatc atgctttgga ctctgatcat
     2281 ctgaccaaaa ctcatcatga tatgtttact aaaggacaag tcaccacagg acagtacagg
     2341 atgcttgcca aaagaggtgg atatgtctgg gttgaaactc aagcaactgt catatataac
     2401 accaagaatt ctcaaccaca gtgcattgta tgtgtgaatt acgttgtgag tggtattatt
     2461 cagcacgact tgattttctc ccttcaacaa acagaatgtg tccttaaacc ggttgaatct
     2521 tcagatatga aaatgactca gctattcacc aaagttgaat cagaagatac aagtagcctc
     2581 tttgacaaac ttaagaagga acctgatgct ttaactttgc tggccccagc cgctggagac
     2641 acaatcatat ctttagattt tggcagcaac gacacagaaa ctgatgacca gcaacttgag
     2701 gaagtaccat tatataatga tgtaatgctc ccctcaccca acgaaaaatt acagaatata
     2761 aatttggcaa tgtctccatt acccaccgct gaaacgccaa agccacttcg aagtagtgct
     2821 gaccctgcac tcaatcaaga agttgcatta aaattagaac caaatccaga gtcactggaa
     2881 ctttctttta ccatgcccca gattcaggat cagacaccta gtccttccga tggaagcact
     2941 agacaaagtt cacctgagcc taatagtccc agtgaatatt gtttttatgt ggatagtgat
     3001 atggtcaatg aattcaagtt ggaattggta gaaaaacttt ttgctgaaga cacagaagca
     3061 aagaacccat tttctactca ggacacagat ttagacttgg agatgttagc tccctatatc
     3121 ccaatggatg atgacttcca gttacgttcc ttcgatcagt tgtcaccatt agaaagcagt
     3181 tccgcaagcc ctgaaagcgc aagtcctcaa agcacagtta cagtattcca gcagactcaa
     3241 atacaagaac ctactgctaa tgccaccact accactgcca ccactgatga attaaaaaca
     3301 gtgacaaaag accgtatgga agacattaaa atattgattg catctccatc tcctacccac
     3361 atacataaag aaactactag tgccacatca tcaccatata gagatactca aagtcggaca
     3421 gcctcaccaa acagagcagg aaaaggagtc atagaacaga cagaaaaatc tcatccaaga
     3481 agccctaacg tgttatctgt cgctttgagt caaagaacta cagttcctga ggaagaacta
     3541 aatccaaaga tactagcttt gcagaatgct cagagaaagc gaaaaatgga acatgatggt
     3601 tcactttttc aagcagtagg aattggaaca ttattacagc agccagacga tcatgcagct
     3661 actacatcac tttcttggaa acgtgtaaaa ggatgcaaat ctagtgaaca gaatggaatg
     3721 gagcaaaaga caattatttt aataccctct gatttagcat gtagactgct ggggcaatca
     3781 atggatgaaa gtggattacc acagctgacc agttatgatt gtgaagttaa tgctcctata
     3841 caaggcagca gaaacctact gcagggtgaa gaattactca gagctttgga tcaagttaac
     3901 tgacaattct gcagatatcc atcacagtgg tcgaccctgt ggaatgtgtg tcagttaggg
     3961 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4021 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4081 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     4141 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4201 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4261 ctaggctttt gcaaaaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
     4321 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     4381 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     4441 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     4501 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     4561 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     4621 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     4681 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     4741 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     4801 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     4861 atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg
     4921 agcgcacgac cccatgcatc gataaaataa aagattttat ttagtctcca gaaaaagggg
     4981 ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag
     5041 gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg aacagatgga
     5101 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     5161 ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc
     5221 tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt
     5281 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
     5341 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
     5401 caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc
     5461 cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt
     5521 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcacatgca
     5581 gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat ggccatagtt
     5641 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5701 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5761 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5821 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5881 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5941 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6001 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6061 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6121 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6181 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6241 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6301 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6361 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6421 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6481 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6541 attatcaaaa aggatcttca cctagatcct tttgcggccg caaatcaatc taaagtatat
     6601 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6661 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     6721 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     6781 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     6841 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6901 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     6961 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7021 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7081 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7141 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7201 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7261 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7321 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7381 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7441 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7501 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7561 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgcagcct
     7621 gaa
//
