LOCUS Exported 7326 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCMV5 TrkA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7326) AUTHORS Yano H, Cong F, Birge RB, Goff SP, Chao MV TITLE Association of the Abl tyrosine kinase with the Trk nerve growth factor receptor. JOURNAL J Neurosci Res. 2000 Feb 1. 59(3):356-64. PUBMED 10679771 REFERENCE 2 (bases 1 to 7326) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7326 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 42..421 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 422..625 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 576..596 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" polyA_signal 3391..4013 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3590..3609) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" primer_bind complement(4037..4057) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 4042..4371 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4222..4357 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4284..4303 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(4409..4428) /label=T7 /note="T7 promoter, forward primer" promoter complement(4410..4428) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4442..4458) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4442..4458) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4455..4477) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4466..4482 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4490..4520) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4535..4556 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4673..4690) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4844..5432) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4924..4943) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5603..6463) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6226..6245 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6464..6568) /gene="bla" /label=AmpR promoter rep_origin 6595..7050 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6682..6701) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6892..6913 /label=F1ori-F /note="F1 origin, forward primer" primer_bind 7176..7198 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 7190..7207 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 7191..7207 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag 61 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt 121 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac 181 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg 241 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag 301 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat 361 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat 421 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt 481 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga 541 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg 601 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagaattccg cgcacatgtc 661 gggggaggcc tggcagctgc agctgggagc gcacagacgg ctgccccgcc tgagcgaggc 721 gggcgccgcc gcgatgctgc gaggcggacg gcgcgggcag cttggctggc acagctgggc 781 tgcggggccg ggcagcctgc tggcttggct gatactggca tctgcgggcg ccgcaccctg 841 ccccgatgcc tgctgccccc acggctcctc gggactgcga tgcacccggg atggggccct 901 ggatagcctc caccacctgc ccggcgcaga gaacctgact gagctctaca tcgagaacca 961 gcagcatctg cagcatctgg agctccgtga tctgaggggc ctgggggagc tgagaaacct 1021 caccatcgtg aagagtggtc tccgtttcgt ggcgccagat gccttccatt tcactcctcg 1081 gctcagtcgc ctgaatctct ccttcaacgc tctggagtct ctctcctgga aaactgtgca 1141 gggcctctcc ttacaggaac tggtcctgtc ggggaaccct ctgcactgtt cttgtgccct 1201 gcgctggcta cagcgctggg aggaggaggg actgggcgga gtgcctgaac agaagctgca 1261 gtgtcatggg caagggcccc tggcccacat gcccaatgcc agctgtggtg tgcccacgct 1321 gaaggtccag gtgcccaatg cctcggtgga tgtgggggac gacgtgctgc tgcggtgcca 1381 ggtggagggg cggggcctgg agcaggccgg ctggatcctc acagagctgg agcagtcagc 1441 cacggtgatg aaatctgggg gtctgccatc cctggggctg accctggcca atgtcaccag 1501 tgacctcaac aggaagaact tgacgtgctg ggcagagaac gatgtgggcc gggcagaggt 1561 ctctgttcag gtcaacgtct ccttcccggc cagtgtgcag ctgcacacgg cggtggagat 1621 gcaccactgg tgcatcccct tctctgtgga tgggcagccg gcaccgtctc tgcgctggct 1681 cttcaatggc tccgtgctca atgagaccag cttcatcttc actgagttcc tggagccggc 1741 agccaatgag accgtgcggc acgggtgtct gcgcctcaac cagcccaccc acgtcaacaa 1801 cggcaactac acgctgctgg ctgccaaccc cttcggccag gcctccgcct ccatcatggc 1861 tgccttcatg gacaaccctt tcgagttcaa ccccgaggac cccatccctg acactaacag 1921 cacatctgga gacccggtgg agaagaagga cgaaacacct tttggggtct cggtggctgt 1981 gggcctggcc gtctttgcct gcctcttcct ttctacgctg ctccttgtgc tcaacaaatg 2041 tggacggaga aacaagtttg ggatcaaccg cccggctgtg ctggctccag aggatgggct 2101 ggccatgtcc ctgcatttca tgacattggg tggcagctcc ctgtccccca ccgagggcaa 2161 aggctctggg ctccaaggcc acatcatcga gaacccacaa tacttcagtg atgcctgtgt 2221 tcaccacatc aagcgccggg acatcgtgct caagtgggag ctgggggagg gcgcctttgg 2281 gaaggtcttc cttgctgagt gccacaacct cctgcctgag caggacaaga tgctggtggc 2341 tgtcaaggca ctgaaggagg cgtccgagag tgctcggcag gacttccaac gtgaggctga 2401 gctgctcacc atgctgcagc accagcacat cgtgcgcttc ttcggcgtct gcaccgaggg 2461 ccgccccctg ctcatggtct ttgagtatat gcggcacggg gacctcaacc gcttcctccg 2521 atcccatgga cctgatgcca agctgctggc tggtggggag gatgtggctc caggccccct 2581 gggtctgggg cagctgctgg ccgtggctag ccaggtcgct gcggggatgg tgtacctggc 2641 gggtctgcat tttgtgcacc gggacctggc cacacgcaac tgtctagtgg gccagggact 2701 ggtggtcaag attggtgatt ttggcatgag cagggatatc tacagcaccg actattaccg 2761 tgtgggaggc cgcaccatgc tgcccattcg ctggatgccg cccgagagca tcctgtaccg 2821 taagttcacc accgagagcg acgtgtggag cttcggcgtg gtgctctggg agatcttcac 2881 ctacggcaag cagccctggt accagctctc caacacggag gcaatcgact gcatcacgca 2941 gggacgtgag ttggagcggc cacgtgcctg cccaccagag gtctacgcca tcatgcgggg 3001 ctgctggcag cgggagcccc agcaacgcca cagcatcaag gatgtgcacg cccggctgca 3061 agccctggcc caggcacctc ctgtctacct ggatgtcctg ggctaggggg ccggcccagg 3121 ggctgggagt ggttagccgg aatactgggg cctgccctca gcatccccca tagctcccag 3181 cagccccagg gtgatctcaa agtatctaat tcaccctcag catgtgggaa gggacaggtg 3241 ggggctggga gtagaggatg ttcctgcttc tctaggcaag gtcccgtcat agcaattata 3301 tttattatcc cttgaaaaaa aaggaattca gatctggtac cacgcgtatc gataagcttg 3361 catgcctgca ggtcgactct agaggatccc gggtggcatc cctgtgaccc ctccccagtg 3421 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta 3481 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg 3541 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa 3601 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa 3661 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc 3721 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc 3781 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg 3841 tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac 3901 gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg 3961 cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg 4021 cggccagctt ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 4081 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 4141 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 4201 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 4261 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 4321 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcctc 4381 gaggaactga aaaaccagaa agttaattcc ctatagtgag tcgtattaaa ttcgtaatca 4441 tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 4501 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 4681 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 4741 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 4801 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 4861 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 4921 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 4981 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 5041 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 5101 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 5161 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 5221 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 5281 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 5341 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 5401 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 5461 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 5521 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 5581 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 5641 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 5701 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 5761 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 5821 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 5881 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 5941 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 6001 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 6061 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 6121 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 6181 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 6241 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 6301 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 6361 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 6421 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 6481 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 6541 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc 6601 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 6661 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 6721 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 6781 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 6841 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 6901 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 6961 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 7021 attttaacaa aatattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg 7081 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 7141 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 7201 ggccagtgcc aagctgatct atacattgaa tcaatattgg caattagcca tattagtcat 7261 tggttatata gcataaatca atattggcta ttggccattg catacgttgt atctatatca 7321 taatat //