LOCUS Exported 10859 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS E[beta]C SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10859) AUTHORS Fuerer C, Nusse R TITLE Lentiviral vectors to probe and manipulate the wnt signaling pathway. JOURNAL PLoS One. 2010 . 5(2):e9370. PUBMED 20186325 REFERENCE 2 (bases 1 to 10859) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10859 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 50..67 /label=L4440 /note="L4440 vector, forward primer" protein_bind 184..205 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 220..250 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 258..274 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 263..285 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 282..298 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 282..298 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 317..337 /label=T3 /note="T3 promoter, forward primer" promoter 319..337 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 365..591 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 592..772 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 819..944 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1437..1670 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1855..1899 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2166..2282 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2318..3496 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2549..3487 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3444..3464 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" promoter 3513..3531 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 3657..3692 /codon_start=1 /product="nanobody epitope tag corresponding to amino acids 16-27 of human beta-catenin (Traenkle et al., 2015)" /label=BC2 tag /translation="PDRKAAVSHWQQ" primer_bind complement(6006..6026) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 6011..6340 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6191..6326 /label=SV40 ori /note="SV40 origin of replication" CDS 6350..7060 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(6495..6513) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 6763..6782 /label=mCherry-F /note="mCherry, forward primer" misc_feature 7067..7655 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7120..7140) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7538..7549) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7742..7975 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 8047..8168 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8084..8103) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8138..8157 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 8208..8343 /label=SV40 ori /note="SV40 origin of replication" promoter complement(8364..8382) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(8392..8409) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(8392..8408) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8401..8423) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 8550..9005 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8637..8656) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8847..8868 /label=F1ori-F /note="F1 origin, forward primer" promoter 9031..9135 /gene="bla" /label=AmpR promoter CDS 9136..9996 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9354..9373) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10167..10755 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10656..10675 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 61 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 121 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 181 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc 241 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca 301 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc 361 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa 421 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac 481 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa 541 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc 601 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 661 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 721 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc 781 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc 841 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt 901 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga 961 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat 1021 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt 1081 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag 1141 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa 1201 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa 1261 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg 1321 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1381 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1441 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1501 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1561 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1621 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1681 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1741 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1801 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1861 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 1921 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 1981 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2041 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2101 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg 2161 ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac 2221 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa attcaaaatt 2281 ttatcggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc 2341 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg 2401 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 2461 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 2521 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg 2581 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt 2641 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta 2701 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt 2761 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta 2821 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg 2881 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc 2941 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg 3001 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc 3061 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc 3121 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc 3181 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga 3241 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac 3301 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt 3361 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt 3421 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc 3481 atttcaggtg tcgtgaggaa ttagcttggt actaatacga ctcactatag ggagacccaa 3541 gctggctagg taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg 3601 cagataacac catggctact caagctgacc tgatggagtt ggacatggcc atggagccgg 3661 acagaaaagc tgctgtcagc cactggcagc agcagtctta cttggatgct ggaatccatg 3721 ctggtgccac cgccacagct cctgccctga gtggcaaggg caaccctgag gaagaagatg 3781 ttgacacctc ccaagtcctt tatgaatggg agcaaggctt ttcccagtcc ttcacgcaag 3841 agcaagtagc tgatattgac gggcagtatg caatgactag ggctcagagg gtccgagctg 3901 ccatgttccc tgagacgcta gatgagggca tgcagatccc atccacgcag tttgacgctg 3961 ctcatcccac taatgtccag cgcttggctg aaccatcaca gatgttgaaa catgcagttg 4021 tcaatttgat taactatcag gatgacgcgg aacttgccac acgtgcaatt cctgagctga 4081 caaaactgct aaacgatgag gaccaggtgg tagttaataa agctgctgtt atggtccatc 4141 agctttccaa aaaggaagct tccagacatg ccatcatgcg ctcccctcag atggtgtctg 4201 ccattgtacg caccatgcag aatacaaatg atgtagagac agctcgttgt actgctggga 4261 ctctgcacaa cctttctcac caccgcgagg gcttgctggc catctttaag tctggtggca 4321 tcccagcgct ggtgaaaatg cttgggtcac cagtggattc tgtactgttc tacgccatca 4381 cgacactgca taatctcctg ctccatcagg aaggagctaa aatggcagtg cgcctagctg 4441 gtggactgca gaaaatggtt gctttgctca acaaaacaaa cgtgaaattc ttggctatta 4501 caacagactg ccttcagatc ttagcttatg gcaatcaaga gagcaagctc atcattctgg 4561 ccagtggtgg accccaagcc ttagtaaaca taatgaggac ctacacttat gagaagcttc 4621 tgtggaccac aagcagagtg ctgaaagtgc tgtctgtctg ctctagcaac aagccggcca 4681 ttgtagaagc tggtgggatg caggcactgg ggcttcatct gacagaccca agtcagcgac 4741 ttgttcaaaa ctgtctttgg actctcagaa acctttcaga tgcagcgact aagcaggaag 4801 ggatggaagg cctccttggg actctagtgc agcttctggg ttccgatgat ataaatgtgg 4861 tcacctgtgc agctggaatt ctctctaacc tcacttgcaa taattacaaa aacaagatga 4921 tggtgtgcca agtgggtggc atagaggctc ttgtacgcac cgtccttcgt gctggtgaca 4981 gggaagacat cactgagcct gccatctgtg ctcttcgtca tctgaccagc cggcatcagg 5041 aagccgagat ggcccagaat gccgttcgcc ttcattatgg actgcctgtt gtggttaaac 5101 tcctgcaccc accatcccac tggcctctga taaaggcaac tgttggattg attcgaaacc 5161 ttgccctttg cccagcaaat catgcgcctt tgcgggaaca gggtgctatt ccacgactag 5221 ttcagctgct tgtacgagca catcaggaca cccaacggcg cacctccatg ggtggaacgc 5281 agcagcagtt tgtggagggc gtgcgcatgg aggagatagt agaagggtgt actggagctc 5341 tccacatcct tgctcgggac gttcacaacc ggattgtaat ccgaggactc aataccattc 5401 cattgtttgt gcagttgctt tattctccca ttgaaaatat ccaaagagta gctgcagggg 5461 tcctctgtga acttgctcag gacaaggagg ctgcagaggc cattgaagct gagggagcca 5521 cagctcccct gacagagtta ctccactcca ggaatgaagg cgtggcaaca tacgcagctg 5581 ctgtcctatt ccgaatgtct gaggacaagc cacaggatta caagaagcgg ctttcagtcg 5641 agctgaccag ttccctcttc aggacagagc caatggcttg gaatgagact gcagatcttg 5701 gactggacat tggtgcccag ggagaagccc ttggatatcg ccaggatgat cccagctacc 5761 gttcttttca ctctggtgga tacggccagg atgccttggg gatggaccct atgatggagc 5821 atgagatggg tggccaccac cctggtgctg actatccagt tgatgggctg cctgatctgg 5881 gacacgccca ggacctcatg gatgggctgc ccccaggtga tagcaatcag ctggcctggt 5941 ttgatactga cctgtaatct agaggatccg gactagttaa cgcgtcgacc agtggtcgac 6001 cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 6061 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 6121 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 6181 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 6241 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 6301 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttacca tggtgagcaa 6361 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga 6421 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga 6481 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga 6541 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat 6601 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt 6661 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat 6721 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa 6781 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg 6841 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac 6901 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt 6961 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg 7021 ccgccactcc accggcggca tggacgagct gtacaagtaa gtcgacaatc aacctctgga 7081 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 7141 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 7201 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 7261 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 7321 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 7381 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 7441 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 7501 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 7561 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 7621 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta 7681 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 7741 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct 7801 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 7861 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 7921 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 7981 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 8041 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 8101 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 8161 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa 8221 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 8281 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 8341 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt 8401 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 8461 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 8521 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc 8581 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 8641 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 8701 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 8761 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 8821 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 8881 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 8941 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac 9001 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 9061 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 9121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 9181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 9241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 9301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 9361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 9421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 9481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 9541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 9601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 9661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 9721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 9781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 9841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 9901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 9961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 10021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 10081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 10141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 10201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 10261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 10321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 10381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 10441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 10501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 10561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 10621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 10681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 10741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 10801 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattac //