LOCUS Exported 5773 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Myc-TEAD4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5773) AUTHORS Li Z, Zhao B, Wang P, Chen F, Dong Z, Yang H, Guan KL, Xu Y TITLE Structural insights into the YAP and TEAD complex. JOURNAL Genes Dev. 2010 Feb 1;24(3):235-40. PUBMED 20123905 REFERENCE 2 (bases 1 to 5773) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5773 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 55..76 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 91..121 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 129..145 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 134..156 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 153..169 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 153..169 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" enhancer 198..577 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 578..781 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 732..752 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 778..802 /label=LNCX /note="Human CMV promoter, forward primer" promoter 1012..1030 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 1012..1029 /label=SP6 /note="SP6 promoter, forward primer" primer_bind 1043..1062 /label=pMT2-F /note="Synthetic intron, forward primer" CDS 1111..1140 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" regulatory 2257..2266 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 2270..2404 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2307..2326) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2361..2380 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" promoter 2473..2830 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2496..2516) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2681..2816 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2743..2762 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(2850..2867) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(2850..2866) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2859..2881) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 3079..3534 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3166..3185) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3376..3397 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3551..3570) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3670..3692 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(3730..3748) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 3816..3920 /gene="bla" /label=AmpR promoter CDS 3921..4781 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4139..4158) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 4952..5540 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5441..5460 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5694..5711 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt 61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt 121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa 181 ttaattcgag ctcgcccgac attgattatt gactagttat taatagtaat caattacggg 241 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 301 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 361 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 421 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga 481 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg 541 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat 601 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 661 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc 721 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 781 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag 841 aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa cgcggattcc 901 ccgtgccaag agtgacgtaa gtaccgccta tagagtctat aggcccaccc ccttggcttc 961 gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg 1021 acactataga ataacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa 1081 ctgcacctcg gttctatcga tcccaccatg gagcagaagc tgatcagcga ggaggacctg 1141 ctcgagggat cctatggtcg gaacgagctg attgcccgct acatcaagct ccggacaggg 1201 aagacccgca ccaggaagca ggtctccagc cacatccagg tgctggctcg tcgcaaagct 1261 cgcgagatcc aggccaagct aaaggaccag gcagctaagg acaaggccct gcagagcatg 1321 gctgccatgt cgtctgcaca gatcatctcc gccacggcct tccacagtag catggccctc 1381 gcccggggcc ccggccgccc agcagtctca gggttttggc aaggagcttt gccaggccaa 1441 gccggaacgt cccatgatgt gaagcctttc tctcagcaaa cctatgctgt ccagcctccg 1501 ctgcctctgc cagggtttga gtctcctgca gggcccgccc catcgccctc tgcgcccccg 1561 gcacccccat ggcagggccg cagcgtggcc agctccaagc tctggatgtt ggagttctct 1621 gccttcctgg agcagcagca ggacccggac acgtacaaca agcacctgtt cgtgcacatt 1681 ggccagtcca gcccaagcta cagcgacccc tacctcgaag ccgtggacat ccgccaaatc 1741 tatgacaaat tcccggagaa aaagggtgga ctcaaggatc tcttcgaacg gggaccctcc 1801 aatgcctttt ttcttgtgaa gttctgggca gacctcaaca ccaacatcga ggatgaaggc 1861 agctccttct atggggtctc cagccagtat gagagccccg agaacatgat catcacctgc 1921 tccacgaagg tctgctcttt cggcaagcag gtggtggaga aagttgagac agagtatgct 1981 cgctatgaga atggacacta ctcttaccgc atccaccggt ccccgctctg tgagtacatg 2041 atcaacttca tccacaagct caagcacctc cctgagaagt acatgatgaa cagcgtgctg 2101 gagaacttca ccatcctgca ggtggtcacc aacagagaca cacaggagac cttgctgtgc 2161 attgcctatg tctttgaggt gtcagccagt gagcacgggg ctcagcacca catctacagg 2221 ctggtgaaag aatgagaatt ctagattaga agcttggccg ccatggccca acttgtttat 2281 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 2341 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 2401 gatcgggaat taattcggcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg 2461 gttaggtacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg 2521 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 2581 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 2641 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 2701 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 2761 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 2821 cttttgcaaa aagctgttaa cagcttggca ctggccgtcg ttttacaacg tcgtgactgg 2881 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 2941 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 3001 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata 3061 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 3121 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 3181 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 3241 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg 3301 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 3361 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 3421 gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 3481 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt 3541 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa 3601 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 3661 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 3721 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 3781 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 3841 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 3901 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 3961 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 4021 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 4081 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 4141 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 4201 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 4261 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 4321 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 4381 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 4441 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 4501 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 4561 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 4621 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 4681 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 4741 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 4801 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 4861 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 4921 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 4981 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 5041 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 5101 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 5161 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 5221 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 5281 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 5341 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 5401 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 5461 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 5521 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 5581 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 5641 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 5701 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 5761 cgattcatta atg //