LOCUS Exported 4457 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBiFC-bJunVN155(I152L) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4457) AUTHORS Kodama Y, Hu CD TITLE An improved bimolecular fluorescence complementation assay with a high signal-to-noise ratio. JOURNAL Biotechniques. 2010 Nov . 49(5):793-805. PUBMED 21091444 REFERENCE 2 (bases 1 to 4457) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4457 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 289..753 /codon_start=1 /product="improved N-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /label=VN155(I152L) /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA" primer_bind complement(334..355) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(595..614) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" polyA_signal 768..902 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(792..811) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 846..865 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(1013..1029) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1013..1029) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1026..1048) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1037..1053 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1061..1091) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1106..1127 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(1244..1261) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1415..2003) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1495..1514) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2174..3034) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2797..2816 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3035..3139) /gene="bla" /label=AmpR promoter primer_bind 3207..3225 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(3263..3285) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 3385..3404 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3598..3620 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3612..3629 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3613..3629 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" enhancer 3656..3959 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 3960..4163 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 4114..4134 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 4160..4184 /label=LNCX /note="Human CMV promoter, forward primer" intron 4301..4397 /label=SV40 intron /note="modified SV40 intron with splice donor and acceptor sites" ORIGIN 1 atggcatcaa tgcagaagct gatctcagag gaggacctgc ttatggccat ggaggcccga 61 attcggaagg cggagaggaa gcgcatgagg aaccgcatcg ctgcctccaa gtgccggaaa 121 aggaagctgg agcggatcgc ccggctagag gaaaaagtga aaaccttgaa agcgcaaaac 181 tccgagctgg cgtccacggc caacatgctc agggaacagg tggcacagct taaacagaaa 241 gtcatgaacc acggtaccgg aggtggcggg agcggaggtg gcgggagtat ggtgagcaag 301 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 361 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 421 ctgaagctga tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 481 ctgggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc 541 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 601 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 661 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 721 aactacaaca gccacaacgt ctatctcacc gcctgagcgg ccgcggggat ccagacatga 781 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta 841 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 901 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt 961 tttcggatcc tctagagtcg atctgcaggc atgctagctt ggcgtaatca tggtcatagc 1021 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 1081 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 1141 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 1201 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 1261 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 1321 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 1381 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 1441 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 1501 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 1561 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 1621 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 1681 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 1741 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 1801 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 1861 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 1921 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 1981 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 2041 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 2101 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 2161 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 2221 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 2281 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 2341 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 2401 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 2461 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 2521 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 2581 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 2641 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 2701 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 2761 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 2821 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 2881 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 2941 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 3001 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 3061 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 3121 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca 3181 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc 3241 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 3301 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 3361 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata 3421 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc 3481 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc 3541 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc 3601 agtcacgacg ttgtaaaacg acggccagtg agttcgagct tgcatgcctg caggtcgtta 3661 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt 3721 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg 3781 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta 3841 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga 3901 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg 3961 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc 4021 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact 4081 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt 4141 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc 4201 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg actctagagg 4261 atccggtact agaggaactg aaaaaccaga aagttaactg gtaagtttag tctttttgtc 4321 ttttatttca ggtcccggat ccggtggtgg tgcaaatcaa agaactgctc ctcagtggat 4381 gttgccttta cttctaggcc tgtacggaag tgttacttct gctctaaaag ctgcggaatt 4441 gtacccgcgg gcccacc //