LOCUS       Exported                9258 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pQCXIH-Myc-YAP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9258)
  AUTHORS   Zhao B, Wei X, Li W, Udan RS, Yang Q, Kim J, Xie J, Ikenoue T, Yu J,
            Li L, Zheng P, Ye K, Chinnaiyan A, Halder G, Lai ZC, Guan KL
  TITLE     Inactivation of YAP oncoprotein by the Hippo pathway is involved in 
            cell contact inhibition and tissue growth control.
  JOURNAL   Genes Dev. 2007 Nov 1. 21(21):2747-61.
  PUBMED    17974916
REFERENCE   2  (bases 1 to 9258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9258
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        2..381
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..585
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             586..761
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    824..1023
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1224..1640
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1570..1592
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1608..1624
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1677..1980
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1981..2184
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2181..2205
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2329..2358
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    3970..4432
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4026..4043)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4253..4272
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4471..5490
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     LTR             6037..6462
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6565..6587)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6723..6743)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6728..7057
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6908..7043
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6970..6989
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7079..7098
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7179..7196)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7350..7938)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7430..7449)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8109..8969)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8732..8751
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8970..9074)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9142..9160
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
       61 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      121 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      181 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      241 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      301 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      361 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      421 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      481 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      541 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcaata aaagagccca
      601 caacccctca ctcggcgcgc cagtcttccg atagactgcg tcgcccgggt acccgtattc
      661 ccaataaagc ctcttgctgt ttgcatccga atcgtggtct cgctgttcct tgggagggtc
      721 tcctctgagt gattgactac ccacgacggg ggtctttcat ttgggggctc gtccgggatt
      781 tggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg ccagcaactt
      841 atctgtgtct gtccgattgt ctagtgtcta tgtttgatgt tatgcgcctg cgtctgtact
      901 agttagctaa ctagctctgt atctggcgga cccgtggtgg aactgacgag ttctgaacac
      961 ccggccgcaa ccctgggaga cgtcccaggg actttggggg ccgtttttgt ggcccgacct
     1021 gaggaaggga gtcgatgtgg aatccgaccc cgtcaggata tgtggttctg gtaggagacg
     1081 agaacctaaa acagttcccg cctccgtctg aatttttgct ttcggtttgg aaccgaagcc
     1141 gcgcgtcttg tctgctgcag cgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt
     1201 ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag
     1261 gtcactggaa agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac
     1321 gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg
     1381 gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc
     1441 atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc
     1501 ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc
     1561 cgtctctccc ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc
     1621 tcactccttc tctaggcgcc ggaattgaag atctggggga tcgatcctct agagtccgtt
     1681 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     1741 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     1801 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     1861 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     1921 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     1981 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     2041 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     2101 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     2161 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     2221 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccgggaa
     2281 cggtgcattg gaacggacct gcaggaattg atccgcggcc gcaccatgga acaaaaactc
     2341 atctcagaag aggatctggc gggcaaggat cccgggcagc agccgccgcc tcaaccggcc
     2401 ccccagggcc aagggcagcc gccttcgcag cccccgcagg ggcagggccc gccgtccgga
     2461 cccgggcaac cggcacccgc ggcgacccag gcggcgccgc aggcaccccc cgccgggcat
     2521 cagatcgtgc acgtccgcgg ggactcggag accgacctgg aggcgctctt caacgccgtc
     2581 atgaacccca agacggccaa cgtgccccag accgtgccca tgaggctccg gaagctgccc
     2641 gactccttct tcaagccgcc ggagcccaaa tcccactccc gacaggccag tactgatgca
     2701 ggcactgcag gagccctgac tccacagcat gttcgagctc attcctctcc agcttctctg
     2761 cagttgggag ctgtttctcc tgggacactg acccccactg gagtagtctc tggcccagca
     2821 gctacaccca cagctcagca tcttcgacag tcttcttttg agatacctga tgatgtacct
     2881 ctgccagcag gttgggagat ggcaaagaca tcttctggtc agagatactt cttaaatcac
     2941 atcgatcaga caacaacatg gcaggacccc aggaaggcca tgctgtccca gatgaacgtc
     3001 acagccccca ccagtccacc agtgcagcag aatatgatga actcggcttc aggtcctctt
     3061 cctgatggat gggaacaagc catgactcag gatggagaaa tttactatat aaaccataag
     3121 aacaagacca cctcttggct agacccaagg cttgaccctc gttttgccat gaaccagaga
     3181 atcagtcaga gtgctccagt gaaacagcca ccacccctgg ctccccagag cccacaggga
     3241 ggcgtcatgg gtggcagcaa ctccaaccag cagcaacaga tgcgactgca gcaactgcag
     3301 atggagaagg agaggctgcg gctgaaacag caagaactgc ttcggcagga gttagccctg
     3361 cgtagccagt taccaacact ggagcaggat ggtgggactc aaaatccagt gtcttctccc
     3421 gggatgtctc aggaattgag aacaatgacg accaatagct cagatccttt ccttaacagt
     3481 ggcacctatc actctcgaga tgagagtaca gacagtggac taagcatgag cagctacagt
     3541 gtccctcgaa ccccagatga cttcctgaac agtgtggatg agatggatac aggtgatact
     3601 atcaaccaaa gcaccctgcc ctcacagcag aaccgtttcc cagactacct tgaagccatt
     3661 cctgggacaa atgtggacct tggaacactg gaaggagatg gaatgaacat agaaggagag
     3721 gagctgatgc caagtctgca ggaagctttg agttctgaca tccttaatga catggagtct
     3781 gttttggctg ccaccaagct agataaagaa agctttctta catggttata gttaattaac
     3841 ggatccggaa ttccgccccc ccccyccctc ccccccccct aacgttactg gccgaagccg
     3901 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     3961 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     4021 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     4081 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     4141 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     4201 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     4261 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     4321 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     4381 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aagcttgcca
     4441 caacccgtac caaagatgga tagatccgga aagcctgaac tcaccgcgac gtctgtcgag
     4501 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     4561 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     4621 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     4681 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
     4741 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     4801 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     4861 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     4921 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     4981 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     5041 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     5101 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     5161 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     5221 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     5281 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     5341 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     5401 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     5461 cccagcactc gtccgagggc aaaggaatag agtagatgcc gaccgaacaa gagctgattt
     5521 cgagaacgcc tcagccagca actcgcgcga gcctagcaag gcaaatgcga gagaacggcc
     5581 ttacgcttgg tggcacagtt ctcgtccaca gttcgctaag ctcgctcggc tgggtcgcgg
     5641 gagggccggt cgcagtgatt caggcccttc tggattgtgt tggtccccag ggcacgattg
     5701 tcatgcccac gcactcgggt gatctgactg atcccgcaga ttggagatcg ccgcccgtgc
     5761 ctgccgattg ggtgcagatc tctatcgagg gctgcagcgc tgcagaggcc gagtgcagaa
     5821 ctgctccaaa gggacctcaa ggctttccga gggacactag gctgactcca tcgagccagt
     5881 gtagagataa gcttatcgat tagtccaatt tgttaaagac aggatatcag tggtccaggc
     5941 tctagttttg actcaacaat atcaccagct gaagcctata gagtacgagc catagataaa
     6001 ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt
     6061 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat
     6121 agagaagttc agatcaaggt caggaacaga tggaacaggg tcgaccctag agaaccatca
     6181 gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa
     6241 tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc
     6301 acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta
     6361 tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc
     6421 tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct cgtccgggat
     6481 cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg gctgcctcgc
     6541 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     6601 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     6661 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt agatccggct
     6721 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6781 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6841 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6901 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6961 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7021 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttactggct taactatgcg
     7081 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     7141 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
     7201 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7261 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7321 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7381 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7441 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7501 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7561 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7621 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7681 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7741 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     7801 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7861 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7921 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7981 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8041 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8101 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8161 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     8221 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8281 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8341 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8401 ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg
     8461 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8521 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8581 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8641 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8701 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta
     8761 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8821 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8881 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8941 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9001 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9061 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     9121 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaatta
     9181 gcttggccat tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt
     9241 ccaacattac cgccatgt
//
