LOCUS Exported 5342 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION mammalian expression of WT c-Jun. ACCESSION . VERSION . KEYWORDS Flag-JunWT-Myc SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5342) AUTHORS Aguilera C, Nakagawa K, Sancho R, Chakraborty A, Hendrich B, Behrens A TITLE c-Jun N-terminal phosphorylation antagonises recruitment of the Mbd3/NuRD repressor complex. JOURNAL Nature. 2011 Jan 13;469(7329):231-5. doi: 10.1038/nature09607. Epub 2011 Jan 2. PUBMED 21196933 REFERENCE 2 (bases 1 to 5342) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5342 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 68..371 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 372..575 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 526..546 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" promoter 621..639 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" regulatory 674..683 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 683..706 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 1742..1771 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" primer_bind complement(1844..1863) /label=T7 /note="T7 promoter, forward primer" promoter complement(1845..1863) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 2137..2258 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2174..2193) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2228..2247 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2265..2720) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2402..2423) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2614..2633 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2747..2851 /gene="bla" /label=AmpR promoter promoter 2853..3210 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2876..2896) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3061..3196 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3123..3142 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3245..4039 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3299..3318) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3909..3928 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4227..4246) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4271..4318 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4647..5235 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5136..5155 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg 61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc 121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt 181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc 241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 601 agcgattacg ccaagctcga aattaaccct cactaaaggg aacaaaagct ggagctccac 661 cgcggtggcg gccgccacca tggattacaa ggatgacgac gataaggccc gggcggatcc 721 cccgggctgc aggaattcga tgactgcaaa gatggaaacg accttctacg acgatgccct 781 caacgcctcg ttcctccagt ccgagagcgg tgcctacggc tacagtaacc ctaagatcct 841 aaaacagagc atgaccttga acctggccga cccggtgggc agtctgaagc cgcacctccg 901 cgccaagaac tcggaccttc tcacgtcgcc cgacgtcggg ctgctcaagc tggcgtcgcc 961 ggagctggag cgcctgatca tccagtccag caatgggcac atcaccacta caccgacccc 1021 cacccagttc ttgtgcccca agaacgtgac cgacgagcag gagggcttcg ccgagggctt 1081 cgtgcgcgcc ctggctgaac tgcatagcca gaacacgctt cccagtgtca cctccgcggc 1141 acagccggtc agcggggcgg gcatggtggc tcccgcggtg gcctcagtag caggcgctgg 1201 cggcggtggt ggctacagcg ccagcctgca cagtgagcct ccggtctacg ccaacctcag 1261 caacttcaac ccgggtgcgc tgagcagcgg cggtggggcg ccctcctatg gcgcggccgg 1321 gctggccttt ccctcgcagc cgcagcagca gcagcagccg cctcagccgc cgcaccactt 1381 gccccaacag atcccggtgc agcacccgcg gctgcaagcc ctgaaggaag agccgcagac 1441 cgtgccggag atgccgggag agacgccgcc cctgtcccct atcgacatgg agtctcagga 1501 gcggatcaag gcagagagga agcgcatgag gaaccgcatt gccgcctcca agtgccggaa 1561 aaggaagctg gagcggatcg ctcggctaga ggaaaaagtg aaaaccttga aagcgcaaaa 1621 ctccgagctg gcatccacgg ccaacatgct cagggaacag gtggcacagc ttaagcagaa 1681 agtcatgaac cacgttaaca gtgggtgcca actcatgcta acgcagcagt tgcaaacgtt 1741 tgagcagaag ctgatctcgg aggaggacct gtgatgaggt cgacctcgag ggggggcccg 1801 gtaccttaat taattaaggt accaggtaag tgtacccaat tcgccctata gtgagtcgta 1861 ttacaattca ctcgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggagatc 1921 caatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt 1981 cacagtccca aggctcattt caggcccctc agtcctcaca gtctgttcat gatcataatc 2041 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg 2101 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat 2161 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 2221 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa 2281 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc 2341 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt 2401 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa 2461 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg 2521 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg 2581 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc 2641 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa 2701 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat 2761 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 2821 aatgcttcaa taatattgaa aaaggaagaa tcctgaggcg gaaagaacca gctgtggaat 2881 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 2941 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 3001 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 3061 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 3121 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 3181 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt 3241 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct 3301 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct 3361 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 3421 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc 3481 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg 3541 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc 3601 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca 3661 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 3721 cgaagaacat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc 3781 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga 3841 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca 3901 ggacatagcg ttggctaccc gtgatattgc tgaagaactt ggcggcgaat gggctgaccg 3961 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 4021 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc 4081 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga 4141 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc 4201 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc 4261 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat 4321 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg 4381 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc 4441 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat 4501 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 4561 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 4621 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 4681 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 4741 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 4801 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 4861 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 4921 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 4981 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 5041 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 5101 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 5161 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 5221 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 5281 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 5341 gc //