LOCUS       Exported                1229 bp ds-DNA     linear   SYN 11-JUN-2021
DEFINITION  Constitutive expression of mutant human Tumor Protein p53 (TP53).
ACCESSION   .
VERSION     .
KEYWORDS    pLX313-TP53-P278A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Giacomelli AO, Yang X, Lintner RE, McFarland JM, Duby M, Kim J, 
            Howard TP, Takeda DY, Ly SH, Kim E, Gannon HS, Hurhula B, Sharpe T, 
            Goodale A, Fritchman B, Steelman S, Vazquez F, Tsherniak A, Aguirre 
            AJ, Doench JG, Piccioni F, Roberts CWM, Meyerson M, Getz G, 
            Johannessen CM, Root DE, Hahn WC
  TITLE     Mutational processes shape the landscape of TP53 mutations in human 
            cancer.
  JOURNAL   Nat Genet. 2018 Oct;50(10):1381-1387. doi: 
            10.1038/s41588-018-0204-y.
  PUBMED    30224644
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(91..132)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    140..164
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgggatcatc tttccaaatt ttgtaatcca gaggttgatt gtcgacttaa cgcgttgatt
       61 gtcgacttaa ccggtactag ttcattacta cgtagaatcg agaccgagga gagggttagg
      121 gataggctta ccgatatcaa ccactttgta caagaaagtt gggcagtctg agtcaggccc
      181 ttctgtcttg aacatgagtt ttttatggcg ggaggtagac tgaccctttt tggacttcag
      241 gtggctggag tgagccctgc tcccccctgg ctccttccca gcctgggcat ccttgagttc
      301 caaggcctca ttcagctctc ggaacatctc gaagcgctca cgcccacgga tctgaagggt
      361 gaaatattct ccatccagtg gtttcttctt tggctgggga gaggagctgg tgttgttggg
      421 cagtgctcgc ttagtgctcc ctgggggcag ctcgtggtga ggctcccctt tcttgcggag
      481 attctcttcc tctgtgcgcc ggtctctccc agcacaggca caaacacgca cctcaaagct
      541 gttccgtccc agtagattac cactggagtc ttccagtgtg atgatggtga ggatgggcct
      601 ccggttcatg ccgcccatgc aggaactgtt acacatgtag ttgtagtgga tggtggtaca
      661 gtcagagcca acctcaggcg gctcataggg caccaccaca ctatgtcgaa aagtgtttct
      721 gtcatccaaa tactccacac gcaaatttcc ttccactcgg ataagatgct gaggaggggc
      781 cagaccatcg ctatctgagc agcgctcatg gtgggggcag cgcctcacaa cctccgtcat
      841 gtgctgtgac tgcttgtaga tggccatggc gcggacgcgg gtgccgggcg ggggtgtgga
      901 atcaacccac agctgcacag ggcaggtctt ggccagttgg caaaacatct tgttgagggc
      961 aggggagtac gtgcagtcac agacttggct gtcccagaat gcaagaagcc cagacgaaac
     1021 cgtagctgcc ctggttaggt ttctgggagg angagatgac agggccagag gctgtgcagg
     1081 cgccgggtaa gaactgctgt gcagggccac cgggaacaac tttggcattc agaactcact
     1141 cgaacttggn ctccatanct ngtcaatcgt cggaacnnna acttcatagc tgacgtaagt
     1201 gnacaanntg tttcatgant acaggtatt
//
