LOCUS       Exported                 821 bp ds-DNA     linear   SYN 13-JUN-2021
DEFINITION  Constitutive expression of sgRNA targeting human TP53.
ACCESSION   .
VERSION     .
KEYWORDS    pXPR003-sgTP53-3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Giacomelli AO, Yang X, Lintner RE, McFarland JM, Duby M, Kim J, 
            Howard TP, Takeda DY, Ly SH, Kim E, Gannon HS, Hurhula B, Sharpe T, 
            Goodale A, Fritchman B, Steelman S, Vazquez F, Tsherniak A, Aguirre 
            AJ, Doench JG, Piccioni F, Roberts CWM, Meyerson M, Getz G, 
            Johannessen CM, Root DE, Hahn WC
  TITLE     Mutational processes shape the landscape of TP53 mutations in human 
            cancer.
  JOURNAL   Nat Genet. 2018 Oct;50(10):1381-1387. doi: 
            10.1038/s41588-018-0204-y.
  PUBMED    30224644
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_RNA        61..136
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    192..309
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        467..678
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 tcnttggctt tatatatctt gtggaaagga cgaaacaccg ccccttgccg tcccaagcaa
       61 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
      121 ggcaccgagt cggtgctttt ttaagcttgg cgtaactaga tcttgagaca aatggcagta
      181 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
      241 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
      301 caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg gctcgagggg
      361 gcccgggtgc aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc
      421 ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca
      481 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgatccgg tgcctagaga
      541 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
      601 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
      661 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
      721 ggttatggcc tttgcgtgcc ttgaattact tccacctggc tgctgtacgt ggattcttga
      781 tcccgagctt cgggttggaa ggggggtggg atggtggagg c
//
