LOCUS Exported 11497 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein lacking the UBA domain of UBAP2L. ACCESSION . VERSION . KEYWORDS pLIX403_deltaUBA_UBAP2L_mCherry SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11497) AUTHORS Markmiller S, Soltanieh S, Server KL, Mak R, Jin W, Fang MY, Luo EC, Krach F, Yang D, Sen A, Fulzele A, Wozniak JM, Gonzalez DJ, Kankel MW, Gao FB, Bennett EJ, Lecuyer E, Yeo GW TITLE Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules. JOURNAL Cell. 2018 Jan 25;172(3):590-604.e13. doi: 10.1016/j.cell.2017.12.032. PUBMED 29373831 REFERENCE 2 (bases 1 to 11497) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11497 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 12..36 /label=LNCX /note="Human CMV promoter, forward primer" protein_bind 60..84 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 3117..3827 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(3262..3280) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 3530..3549 /label=mCherry-F /note="mCherry, forward primer" protein_bind complement(3829..3853) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 3864..3884 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQS" CDS 3885..3908 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 3925..3942 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" promoter 3952..4462 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 4381..4399 /label=hPGK-F /note="Human PGK promoter, forward primer" CDS 4472..5068 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(4472..4491) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 4968..4988 /label=Puro-F /note="Puromycin resistance gene, forward primer" CDS 5069..5122 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 5123..5869 /codon_start=1 /product="improved tetracycline-controlled transactivator" /label=rtTA-Advanced /note="In the Tet-On(R) system, rtTA-Advanced binds to the Tet-responsive element and stimulates transcription only in the presence of tetracycline or doxycycline." /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG" primer_bind complement(5909..5929) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6327..6338) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6543..6776 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 6848..6969 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6885..6904) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6939..6958 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 7009..7144 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7071..7090 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 7144..7215 /gene="bla" /label=AmpR promoter CDS 7216..8076 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7434..7453) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8247..8835 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8736..8755 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8989..9006 /label=L4440 /note="L4440 vector, forward primer" protein_bind 9123..9144 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9159..9189 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9197..9213 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9202..9224 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9221..9237 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9221..9237 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9256..9276 /label=T3 /note="T3 promoter, forward primer" promoter 9258..9276 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 9304..9530 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 9531..9711 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 9758..9883 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 10376..10609 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 10794..10838 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 11051..11168 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 11222..39 /label=tight TRE promoter /note="Tet-responsive promoter PTight, consisting of seven tet operator sequences followed by the minimal CMV promoter" protein_bind 11230..11248 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 11266..11284 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 11301..11319 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 11337..11355 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 11373..11391 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 11408..11426 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 11444..11462 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" ORIGIN 1 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagaattgg ctagcatcaa 61 caagtttgta caaaaaagca ggcttcacca tgatgacatc ggtgggcact aaccgagccc 121 ggggaaactg ggaacaacct caaaacaagg atggtggcca gacggaatcc aatgaggaag 181 gcaaagaaaa tcgagaccgg gacagagact atagtcggcg acgtggtggg ccaccaagac 241 gggggagagg tgccagccgt ggacgagagt ttcgaggtca ggaaaatgga ttggatggca 301 ccaagagtgg agggccttct ggaagaggaa cagaaagagg cagaaggggc cgtggccgag 361 gcagaggtgg ctctggtagg cgaggaggaa ggttttctgc tcaaggaatg ggaaccttta 421 acccagctga ttatgcagag ccagccaata ctgatgataa ctatggcaat agcagcggca 481 atacgtggaa caacactggc cactttgaac cagatgatgg gacgagtgca tggaggactg 541 caacagagga gtgggggact gaagattgga atgaagatct ttctgagacc aagatcttca 601 ctgcctctaa tgtgtcttca gtgcctctgc ctgcggagaa tgtgacaatc actgctggtc 661 agagaattga ccttgctgtt ctgctgggga agacaccatc tacaatggag aatgattcat 721 ctaatctgga tccgtctcag gctccttctc tggcccagcc tctggtgttc agtaattcga 781 agcagactgc catatcacag cctgcttcag ggaacacatt ttctcatcac agtatggtga 841 gcatgttagg gaaaggattt ggtgatgtcg gtgaagctaa aggcggcagt actacaggct 901 cccagttctt ggagcaattc aagactgccc aagccctggc tcagttggca gctcagcatt 961 ctcagtctgg aagcaccacc acctcctctt gggacatggg ctcgacgaca caatccccat 1021 cactggtgca gtatgatttg aagaacccaa gtgattcagc agtgcacagc ccctttacaa 1081 agcgccaggc ttttacccca tcttcaacca tgatggaggt gttccttcag gagaagtcac 1141 ctgcagtggc tacctccaca gctgcacctc cacctccgtc ttctcctctg ccaagcaaat 1201 ccacatcggc tccacagatg tcgcctggat cttcagacaa ccagtcctct agccctcagc 1261 cggctcacca gaaactgaaa cagcagaaga aaaaagcctc cttgacttct aagattcctg 1321 ctctggctgt ggagatgcct ggctcagcag atatctcagg gctaaacctg cagtttgggg 1381 cattgcagtt tgggtcagag cctgtccttt ctgattatga gtccaccccc accacgagcg 1441 cctcttcaag ccaggctcca agtagcctgt ataccagcac ggccagtgaa tcatcctcta 1501 caatttcatc taaccagagt caggagtctg gttatcagag cggcccaatt cagtcgacaa 1561 cctatacctc ccaaaataat gctcagggcc ctctttatga acagagatcc acacagactc 1621 ggcggtaccc cagctccatc tcttcatcac cccaaaagga cctgactcag gcaaagaatg 1681 gcttcagttc tgtgcaggcc acgcagttac agaccacaca atctgttgaa ggtgctacag 1741 gctctgcagt gaaatctgat tcaccttcca cttctagcat cccccctctc aatgaaacgg 1801 tatctgcagc ttccttactg acgacaacca atcagcattc atcctccttg ggtggcttga 1861 gccacagtga ggagattcca aatactacca ccacacaaca cagcagcacg ttatctacgc 1921 agcagaatac cctttcatca tcaacatctt ctgggcgcac ttcgacatcc actcttttgc 1981 acacaagtgt ggagagtgag gcgaatctcc attcttcctc cagcactttt tccaccacat 2041 ccagcacagt ctctgcacct cccccagtgg tcagtgtctc ctccagtctc aatagtggca 2101 gtagcctggg cctcagccta ggcagcaact ccactgtcac agcctcgact cgaagctcag 2161 ttgctacgac ttcaggaaaa gctcctccca acctccctcc tggggtcccg ccgttgttgc 2221 ctaatccgta tattatggct ccagggctgt tacatgccta cccgccacaa gtatatggtt 2281 atgatgactt gcagatgctt cagacaagat ttccattgga ttactacagc atcccatttc 2341 ccacacccac tactccgctg actgggaggg atggtagcct ggccagcaac ccttattctg 2401 gtgacctcac aaagttcggc cgtggggatg cctcctcccc agccccggcc acaaccttgg 2461 cccaacccca acagaaccag acgcagactc accataccac gcagcagaca ttcctgaacc 2521 cggcgctgcc tcctggctac agttacacca gcctgccata ctatacaggg gtcccgggcc 2581 tccccagcac cttccagtat gggcctgctg tgttccctgt ggctcctacc tcttccaagc 2641 agcatggtgt gaatgtcagt gtgaatgcat cggccacccc tttccaacag ccgagtggat 2701 atgggtctca tggatacaac actggtgttt cagtcacctc cagtaacacg ggcgtgccag 2761 atatctcggg ttctgtgtac tccaaaaccc agcagtcctt tgagaaacaa ggttttcatt 2821 ccggtactcc tgctgcttcc ttcaacttgc cttcagccct aggaagtggg ggccccatca 2881 atccggccac agctgctgcc tacccacctg ccccctttat gcacattctg accccccatc 2941 agcagccgca ttctcagatc cttcaccatc acctgcagca ggatggccag acgggcagcg 3001 ggcaacgtag ccagaccagc tccatcccgc agaagcccca gaccaacaag tctgcctaca 3061 acagctacag ctggggggcc aacaccggtg tggacaccgg caacggttcc gctagcatgg 3121 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc 3181 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc 3241 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg 3301 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg 3361 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga 3421 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg 3481 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc 3541 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc 3601 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg 3661 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca 3721 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg 3781 ccgagggccg ccactccacc ggcggcatgg acgagctcta caagtaaaac ccagctttct 3841 tgtacaaagt ggttgcaacg cgtgaaaacc tgtattttca gtccgattac aaggacgacg 3901 atgacaagta ggcatagacc ggtccaccac caccaccacc actaaggatc cggggttggg 3961 gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg 4021 ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag 4081 cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc 4141 cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc 4201 acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc 4261 gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga 4321 aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg 4381 gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg 4441 aatcaccgac ctctctcccc agcaattcac catgaccgag tacaagccca cggtgcgcct 4501 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta 4561 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca 4621 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg 4681 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga 4741 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga 4801 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt 4861 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc 4921 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt 4981 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac 5041 ctggtgcatg acccgcaagc ccggtgccga aggtagaggt tctctcctca cttgtggtga 5101 tgttgaagaa aaccctggtc caatgtctag actggacaag agcaaagtca taaacggagc 5161 tctggaatta ctcaatggtg tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa 5221 gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga 5281 tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga 5341 gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca 5401 tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct 5461 ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc 5521 tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt 5581 agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc 5641 aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat 5701 catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg acgcccttga 5761 cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct 5821 gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaac taagtaagga 5881 tcgatccaag atatcgtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 5941 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 6001 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 6061 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 6121 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 6181 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 6241 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc 6301 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 6361 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 6421 tctccctttg ggccgcctcc ccgcctgttt cgcctcggcg tccggactag aggtaccttt 6481 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 6541 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc 6601 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 6661 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 6721 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 6781 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 6841 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 6901 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 6961 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta 7021 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 7081 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 7141 ggcctttcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 7201 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 7261 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 7321 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 7381 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 7441 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 7501 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 7561 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 7621 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 7681 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 7741 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 7801 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 7861 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 7921 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 7981 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 8041 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 8101 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 8161 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 8221 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 8281 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 8341 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 8401 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 8461 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 8521 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 8581 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 8641 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 8701 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 8761 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 8821 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 8881 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 8941 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 9001 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 9061 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 9121 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct 9181 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat 9241 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct 9301 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac 9361 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg 9421 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat 9481 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct 9541 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 9601 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 9661 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc 9721 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct 9781 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 9841 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 9901 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 9961 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 10021 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 10081 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 10141 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 10201 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg 10261 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 10321 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 10381 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc 10441 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 10501 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 10561 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 10621 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 10681 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca 10741 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg 10801 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa 10861 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa 10921 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt 10981 ttcagaccca cctcccaacc ccgaggggac aattctcgac ctcgagacaa atggcagtat 11041 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag 11101 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc 11161 aaaattttcg ggtttattac agggacagca gagatccact ttggccgcga atcgatatgt 11221 cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata 11281 gagaacgatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc 11341 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc 11401 gagtttatcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga 11461 gaacgtatgt cgaggtaggc gtgtacggtg ggaggcc //