LOCUS       Exported               13814 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses pentamerised full-length protein ectodomain with no 
            N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 
            d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags. .
ACCESSION   .
VERSION     .
KEYWORDS    RON2-COMP-blac-flag-his
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13814)
  TITLE     Pentamerised full-length protein ectodomains with no N-linked 
            glycans
REFERENCE   2  (bases 1 to 13814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13814
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     310..329
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      352..361
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             7348..8133
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             8140..8205
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             8212..8229
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    8265..8320
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8319..8338)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(10556..10574)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10642..10746
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10747..11607
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11778..12366
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12267..12286
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12520..12537
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12654..12675
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12690..12720
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12728..12744
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12733..12755
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12752..12768
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12752..12768
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        13039..13418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13419..13622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     13573..13593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     13619..13643
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgcctct
      421 gagctgagca agcacgtgaa gcacgacacc gacgagctga agtacgccag ccccacctac
      481 gaccccaaga aaggcaccaa agtgatcttc tacatgcccg gcaacgagca gggcgtgatc
      541 cccaacaaca tccagaacaa gcagcacggc accagcatct accccgccat cgagtacccc
      601 accaccaagt accctgccat tgagtatccc ggcagcgaga tgaacaacgg caagaccggc
      661 accaccaaca gcggcatcta caacaaggcc cacggcagca gcaacgacta caacgccgcc
      721 aacagccagg acaagaccat caacctgcac atcgacgaga acaacgccaa caatgcctac
      781 cagccctacg acagcaacaa tgctaacaac aacgctaact ccaacaataa cgcaaacaat
      841 aatgccaata acaacgcaaa caacaatgca aacaattatg ctaacaacaa tgccaacacg
      901 aacagcaaca ccaactccaa taccaacagt aataacaatg ccgccaatta cgacctgaac
      961 agcgagtacg agaagatccg gcggaaagag gaagaggccg ccagacggat cgagagagag
     1021 cggagagccg acatcaaccg gaagaaccag gataataacg ctaataagta caacaatgag
     1081 cagaacggcg gctacgagag cgacggcaac agccccaaca gcagagtgaa catcaatatc
     1141 gccaacaacg gcgcccacgg aaaccctcac aacaacaacg cctacggcca caagaacaac
     1201 atgcacaata ccgccaacgg caactacgcc aatggcaatt atgcaaacgg aaattacgcc
     1261 aagggcgact acaccaacgg ggattacaca aacggcgatt atacgaatgg ggactatacg
     1321 aacgggatca acaacaatat gcacgggaag aacaactaca acaccgcaaa tggcgagtac
     1381 gtgaacggcg cctatgacgg cctgaacaat ggcgcctaca agctgatcgg caacctgaac
     1441 aacaatcaga acgtggacaa cagctacaac cagggcaacg agaacgacaa gacctacacc
     1501 agcaactaca atatcaacct cgaggacaac aagaacagcc ccgataacaa ccagaactac
     1561 atcagcacct tcaacaagga catcggccct aacaaggccg agcggagcta ctacgacgtg
     1621 tacggcagag agtacgacga caacaagtac aatccgtaca ataaggccaa caaccataac
     1681 acgaaccaga atggcgccac cacctacggc tctaacgcca acggggccta tggccccaac
     1741 ggcgcttacg gcagcaatgg ggcctacgag cccaacgagg cctacggacc taatggcgca
     1801 tacggcccaa atggcgctta tggacacaac gggaagtacg gctccaaggg cacatacggc
     1861 aacaacgagg ctccctacta cgtggaacac cccgagtacg ataacggcaa gtccatgagc
     1921 gacttccacg tgaaggacag caaggacaat atcggccctg gcggcgacta ccctaacctg
     1981 taccagaaca tctacggcaa tgagaagaac cccaacatct tccccggcag ccccagaaac
     2041 atcaacgtgt acagcgtgca ccacatccct aacaacgggg ctaacggcgg cctgaatagc
     2101 ggagccaatg gcggactcaa caatggtgcc aacgatgggc ttaacaatgg ggcaaatggt
     2161 ggcctcaata acggcgcaaa cggtggactt aataatggtg ctaatggagg acttaacaac
     2221 ggaatgaata atggcatgaa caacgggatg aacaacggaa tgaacaatgg gatgaataat
     2281 ggaatgaata acggcatgaa taatggcgcc aacggcggcc ttaacaatgg gatgaacaat
     2341 ggcatgaaca atggaatgaa taatgggatg aacaatggta tccacgacga cctgtacaac
     2401 agcgagaaca gcgctttcaa caacgggctt aataacgccg gcagaaccgg actgaacaac
     2461 gcttacccac acaacggcat gctcaataat ggggccgagt acaacgtgca ctatggcaac
     2521 agcgacagca ataacgccaa tgacgccatg ctgaacgaga actactacag cgactccgac
     2581 tacgacgacc acacccctgg caacaagaaa aaggtgtaca agagcgtggc cgagagaaac
     2641 aagaagtccg cctctcagga cagcctggga gccggcttca gcgatagcga cagcgattct
     2701 gagtacgagg tggtggacgg cgagaacaaa aagtacaaga agaagaacaa agaaaacaac
     2761 gagaaggata agtatgagaa caactgggat agcaacaatt acaatagcga caacgagatc
     2821 aaggacggct acctgagcga gagcgagcgc gagtacgccc ggaacaaggc caacgagatt
     2881 gaggacaaga tgaagaaggg cgagtacagc cggaagtata agaacagcaa gagcaatgag
     2941 gccggctacg ccagcaagca gacctccgac agcgacgact ccgatatcga ggccaacgcc
     3001 ttctatgtgg acaacggcca agagatgctg atcaaagaga aagagcacta cagctccgac
     3061 tccgagcacc acaaagagga aagcgccagc atcggaaatc tgaacgtgtt ctttcccgcc
     3121 gaaaactacc acttcagcac ctacatgggc ttcgaccggc ggagcttcct gcccagcaat
     3181 gagatcgagc tggaaaagat gatcggcgcc aacttcgcca acgaagtgaa gaactactgc
     3241 tcccggcaga atgtggccca gaagatcggc gattacctga atatcgcctt cgagtactcc
     3301 agagccctgg aagaactgag atccgagatg atcctggatt tcaacaagcg gaagcacctg
     3361 acaaacaacg ccgacgacac catcctgcac atgattgaga acgccgagaa gcggaagaac
     3421 gaccctaact acaaagaagc ctacgaaaac aaggactacg caaataatgc taatatcttc
     3481 atgaatgagt acagcaaccc gctgagcacc aagtataaca agatcctgaa agagtacctc
     3541 tgccacctgt tcgtgaacaa ccccggcacc aagcccctgg aaagactgta ctacaacagc
     3601 ctggccctgg gcgagctggt ggaacccatc agaaacaagt tcaagagcct ggccagcagc
     3661 accatcgact tcaactacga gatccacatg gccagcgcct ccaacatcta cctgctggcc
     3721 cacttcctgg tgctgtccct ggcctacctg tcctacaacg agtacttcac caccggcaca
     3781 aagagcttct acagcctgcc taccatcctg accgccaact ccgacaatag cttctttatg
     3841 ctcaacgaga tgtgcaacat ccactacaac ccgaacaaga acttcaagaa ggatatcacc
     3901 ttcatcccca tcgagagccg gcccaagcgg accaccacct tctacggcga gcggcggctg
     3961 acctgcgacc tgctggaact ggtgctgaac gccatcatgc tcatcaacat caatgagatt
     4021 aacaacgttt tcagcaataa taacgtggac ggctatgaga actccctgag cttcagccac
     4081 aacgccatcc gcatcttcag caaagtgtgc cccaagatca acaatgacaa cgtcctgaag
     4141 tgcgagttcg aggaaagcag cctgtacaat cctaagatca tcaagaacga cgccagcgag
     4201 aagtccagcc agaagaacct gaagaaggcc tttgacctgc tgcggaccta cgccgagatc
     4261 gagggccaca gcgccgaggg ctccaccagc ccttactacg tgtccctgat tctggacgac
     4321 atgaagtaca acgacttcta caagtacacc ctttggtacg agccccgcga gctgatctat
     4381 ggcgacatcc ggggcatgca aatgaagaag aaaaaaaaga ccaagtacat ctacaatgac
     4441 tttatgaaga agtctaccca gctgaagaaa aagctgatca agaatgacct caagtacaac
     4501 ctgaagtcca agggcctggt gttcctgtac gccatgatcg ataagtacgg atccatcctg
     4561 aacaaagccc agaaagccaa ggtgcagttc ctgaactccg ccagctccat ccggtactac
     4621 ctgtacctca acaaagtcat cttcaagagc gccaagacat acctggacat catgaagcgg
     4681 gtgctcgaag aactgcagac cagcaccaat acccctctga agttcctcgt gcggggcaac
     4741 tacatcgaga acatcaacaa tattgcccgg aacgacaaca tgttctacgc caacctgttt
     4801 gtgctgaccg ccctgagcag acgggacccc gtgaaggatt actacaacga caaacggaag
     4861 atgctgagcg ccaccctggc cgagaagttc gccaattccg cctccatgct gatccctcac
     4921 aagctgcgga agctggtggt gtccatgaag aaaggcctgc tgaaaaagaa gctgctgacc
     4981 tctctggcca aagtgaagct gctccagcac atccccgccc acatgctgga aaacattgcc
     5041 tccagcatcc ggttcaccac ccacaccatt gccaccatgc agatcatcca gaatgccaag
     5101 tatatgtcca agcacaattt cgcccagtac gatagcaagg gcatgctggc ccggcagatc
     5161 ttcaccaagg gcggcttcgc cgagtatgcc gacaacctga tggccaagtg gttcagcaag
     5221 ggcttcgaag agtacaagag agagcagatc gagaatttca agatggaaaa cagcatcgac
     5281 agcgagctga aagactctga gcgcgaggac gagaatgacg cctccgagga atccgccaag
     5341 aagaaactgc aggatctgca gctggaagaa agggaaaaaa tgaaaaaaga gaacagcctg
     5401 ctgttcaacc aggccgacaa gtgggaccag ttcatcaaca aagaacttgt gcgcgccctg
     5461 ggcctgtggc tggaattcaa cgacaacccc gctaacgccg cctccttcgt gtacaaggtg
     5521 gtcgaggact ccaagcacct cctcgagaac aatatcgata acaatattat cttcagccgg
     5581 accgtgaagc ccaccaagca gacagccttc cggcggttct tcaacaaaat cctgagcctg
     5641 ggcaacatgc tgctgagaaa gcccagcttc agagtggaac acgccctttg gttcggcgcc
     5701 acaatcgaca tcaagaaagc cttcatcctg ctcgagaagg tgtccgagct gcacaaaatg
     5761 ctgaacaacc aggacgagag ctggctgatc aacgaagcct ttatcgagat cgtggatcac
     5821 gtggtggacc tgagcacata caaacacgtg cgcgagccct tcggcgtggc ccggaatccc
     5881 ggaatgatgg ccattaaccc taagtacgcc gagctgagcc acgagaaccg gctgcgcgag
     5941 ctgcagaaca gcatgtgcgc cgaccactgc agcagcgtgt ggaaagtgat cagctccttc
     6001 gccctgcacc acctgaaaaa ccccgacagc ctgcacacct acgagtccaa gttctccaag
     6061 aactccttcg gaaacaagat cgacgacaag gacttcgtgc acaacttcaa aatgattctg
     6121 ggcggcgacg ctgtgctgca ctacttcgac aatctgctgc ccaagaccat gaaaaaggac
     6181 ctcaaggcca tgaagtatgg cgtcagcctg accagcgcct actccctgaa gctgaccaag
     6241 atcattttca gccagatgca gctgccttac ctgagccaga tgttttacat gcaagcccct
     6301 tacttcggcc acttcatcgg caagtggcag aagaagcggc agcagagccg gctgaaagaa
     6361 atcatgagct tcatgaccct gggcagcctg tccgcctaca ccctgttcag cgccatggac
     6421 atcacccagc aggccaagga cattggcgcc ggacccgtgg ccagctgctt taccaccaga
     6481 atgagccctc cccagcagat ctgtctgaat tccgtggtca acaccgccct gtccaccagc
     6541 acccagagcg ctatgaagtg cgttttctcc gtcggcgcgc cgtcgacctc catcacggcc
     6601 tataagagtg agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc
     6661 ctgcagggag agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc
     6721 ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag
     6781 ctgtccgaaa cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt
     6841 tctggcaacc tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg
     6901 gtgatgaaag tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc
     6961 tcacccaaga tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag
     7021 gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa
     7081 ggtgaagagg tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattccggc
     7141 ggcggctccg gaggcggtac cggtggcggc gacctagccc cacagatgct tcgagaactc
     7201 caggagacta atgcggcgct gcaagacgtg agagagctct tgcgacagca ggtcaaggag
     7261 atcaccttcc tgaagaatac ggtgatggaa tgtgacgcct gcggtctaga cagaaacctg
     7321 cctccactag cacctctggg cccacaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7381 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7441 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7501 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     7561 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7621 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7681 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7741 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7801 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7861 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7921 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7981 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8041 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8101 gctgagatag gtgcctcact gattaagcat tggggccctg actacaaaga ccatgacggt
     8161 gattataaag atcatgacat cgattacaag gatgacgatg acaagggccc tcaccaccac
     8221 catcaccact aataataagg atcccccgac ctcgacctct ggctaataaa ggaaatttat
     8281 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     8341 ggcaaatcat ttggtcgaga tccctcggag atctctagct agaggatcga tccccgcccc
     8401 ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag tgcatgtaat
     8461 cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg acacgggggg
     8521 ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg gatgtgcaca
     8581 tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg gactgcaaca
     8641 caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct gggggacatg
     8701 tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc agaggggcct
     8761 gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt ataaggcccc
     8821 cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac tatccagact
     8881 aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga attagggtta
     8941 gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac ggttttattt
     9001 acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg cagtggctga
     9061 agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca ttctccttcg
     9121 tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa
     9181 acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag tggtggcatt
     9241 gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact agtgtaggaa
     9301 tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa gccgtaaaga
     9361 ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct agtgcaatat
     9421 gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt
     9481 acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga ctccactggt
     9541 tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc ataaacaaag
     9601 acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag cccccacacg
     9661 ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat tgtaaccccg
     9721 ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc ggggaatacc
     9781 tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat
     9841 tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc
     9901 gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat
     9961 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
    10021 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
    10081 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
    10141 agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag agattagggt
    10201 agtatatgct atcctaattt atatctgggt agcatatact acccaaatat ctggatagca
    10261 tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagca
    10321 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
    10381 tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca
    10441 tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca
    10501 tatgctatcc tcatgataag ctgtcaaaca tgagaatttt cttgaagacg aaagggcctc
    10561 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
    10621 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
    10681 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
    10741 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
    10801 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
    10861 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
    10921 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
    10981 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
    11041 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
    11101 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
    11161 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
    11221 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
    11281 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
    11341 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
    11401 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    11461 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    11521 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    11581 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    11641 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    11701 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    11761 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    11821 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    11881 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    11941 tagttaggcc accacttcaa gaactttgta gcaccgccta catacctcgc tctgctaatc
    12001 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    12061 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    12121 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    12181 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    12241 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    12301 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    12361 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    12421 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    12481 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    12541 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    12601 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
    12661 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
    12721 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
    12781 aagctctagc tagaggtcga ccaattctca tgtttgacag cttatcatcg cagatccggg
    12841 caacgttgtt gccattgctg caggcgcaga actggtaggt atggaagatc tatacattga
    12901 atcaatattg gcaattagcc atattagtca ttggttatat agcataaatc aatattggct
    12961 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc
    13021 caatatgacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
    13081 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
    13141 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
    13201 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
    13261 cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg
    13321 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt
    13381 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
    13441 ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
    13501 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaataacc
    13561 ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
    13621 ctcgtttagt gaaccgtcag atcctcactc tcttccgcat cgctgtctgc gagggccagc
    13681 tgttgggctc gcggttgagg acaaactctt cgcggtcttt ccagtactct tggatcggaa
    13741 acccgtcggc ctccgaacgg tactccgcca ccgagggacc tgagcgagtc cgcatcgacc
    13801 ggatcggaaa acct
//
