LOCUS       Exported               14458 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Homologous recombination vector for dox-inducible overexpression of 
            OTX2, PAX6, and MITF in CLYBL locus.
ACCESSION   .
VERSION     .
KEYWORDS    CLYBL hOPM
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14458)
  TITLE     TF-induced RPE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14458
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        2..379
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    81..99
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    117..135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    153..171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    189..207
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    225..243
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     288..312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1295..1348
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2666..2719
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    4305..4426
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4342..4361)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4396..4415
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4475..4491)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4475..4491)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4488..4510)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4499..4515
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4523..4553)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4568..4589
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4706..4723)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     5015..5034
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    5033..5088
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     5134..5153
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(5244..5990)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6022..6041)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          6049..7065
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(6103..6125)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     enhancer        7345..7724
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    7730..7954
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7943..7960
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    complement(7976..8009)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     complement(8285..8304)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8554..8572
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      8714..8723
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     LTR             8761..9029
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(9042..9253)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     polyA_signal    9289..9513
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(9534..10133)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9617..9637)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     10114..10133
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(10143..10196)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    10220..10245
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    complement(10252..10285)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             11154..12014
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11372..11391)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12185..12773
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12674..12693
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta tcagtgatag
       61 agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga gtttactccc
      121 tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag aacgtatcta
      181 cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag
      241 agaacgtata agctttaggc gtgtacggtg ggcgcctata aaagcagagc tcgtttagtg
      301 aaccgtcaga tcgcctggag caattccaca acacttttgt cttataccaa ctttccgtac
      361 cacttcctac cctcgtaaag gatccgccgc caccatgatg tcttatctta agcaaccgcc
      421 ttacgcagtc aatgggctga gtctgaccac ttcgggtatg gacttgctgc acccctccgt
      481 gggctacccg gggccctggg cttcttgtcc cgcagccacc ccccggaaac agcgccggga
      541 gaggacgacg ttcactcggg cgcagctaga tgtgctggaa gcactgtttg ccaagacccg
      601 gtacccagac atcttcatgc gagaggaggt ggcactgaaa atcaacttgc ccgagtcgag
      661 ggtgcaggta tggtttaaga atcgaagagc taagtgccgc caacaacagc aacaacagca
      721 gaatggaggt caaaacaaag tgagacctgc caaaaagaag acatctccag ctcgggaagt
      781 gagttcagag agtggaacaa gtggccaatt cactcccccc tctagcacct cagtcccgac
      841 cattgccagc agcagtgctc ctgtgtctat ctggagccca gcttccatct ccccactgtc
      901 agatcccttg tccacctcct cttcctgcat gcagaggtcc tatcccatga cctatactca
      961 ggcttcaggt tatagtcaag gatatgctgg ctcaacttcc tactttgggg gcatggactg
     1021 tggatcatat ttgaccccta tgcatcacca gcttcccgga ccaggggcca cactcagtcc
     1081 catgggtacc aatgcagtca ccagccatct caatcagtcc ccagcttctc tttccaccca
     1141 gggatatgga gcttcaagct tgggttttaa ctcaaccact gattgcttgg attataagga
     1201 ccaaactgcc tcctggaagc ttaacttcaa tgctgactgc ttggattata aagatcagac
     1261 atcctcgtgg aaattccagg ttttgggatc gggagagggc agaggaagtc tgctaacatg
     1321 cggtgacgtc gaggagaatc ctggcccaat gcagaacagt cacagcggag tgaatcagct
     1381 cggtggtgtc tttgtcaacg ggcggccact gccggactcc acccggcaga agattgtaga
     1441 gctagctcac agcggggccc ggccgtgcga catttcccga attctgcaga cccatgcaga
     1501 tgcaaaagtc caagtgctgg acaatcaaaa cgtgtccaac ggatgtgtga gtaaaattct
     1561 gggcaggtat tacgagactg gctccatcag acccagggca atcggtggta gtaaaccgag
     1621 agtagcgact ccagaagttg taagcaaaat agcccagtat aagcgggagt gcccgtccat
     1681 ctttgcttgg gaaatccgag acagattact gtccgagggg gtctgtacca acgataacat
     1741 accaagcgtg tcatcaataa acagagttct tcgcaacctg gctagcgaaa agcaacagat
     1801 gggcgcagac ggcatgtatg ataaactaag gatgttgaac gggcagaccg gaagctgggg
     1861 cacccgccct ggttggtatc cggggacttc ggtgccaggg caacctacgc aagatggctg
     1921 ccagcaacag gaaggagggg gagagaatac caactccatc agttccaacg gagaagattc
     1981 agatgaggct caaatgcgac ttcagctgaa gcggaagctg caaagaaata gaacatcctt
     2041 tacccaagag caaattgagg ccctggagaa agagtttgag agaacccatt atccagatgt
     2101 gtttgcccga gaaagactag cagccaaaat agatctacct gaagcaagaa tacaggtatg
     2161 gttttctaat cgaagggcca aatggagaag agaagaaaaa ctgaggaatc agagaagaca
     2221 ggccagcaac acacctagtc atattcctat cagcagtagt ttcagcacca gtgtctacca
     2281 accaattcca caacccacca caccggtttc ctccttcaca tctggctcca tgttgggccg
     2341 aacagacaca gccctcacaa acacctacag cgctctgccg cctatgccca gcttcaccat
     2401 ggcaaataac ctgcctatgc aacccccagt ccccagccag acctcctcat actcctgcat
     2461 gctgcccacc agcccttcgg tgaatgggcg gagttatgat acctacaccc ccccacatat
     2521 gcagacacac atgaacagtc agccaatggg cacctcgggc accacttcaa caggactcat
     2581 ttcccctggt gtgtcagttc cagttcaagt tcccggaagt gaacctgata tgtctcaata
     2641 ctggccaaga ttacagggat cgggagaggg cagaggaagt ctgctaacat gcggtgacgt
     2701 cgaggagaat cctggcccaa tgcagtccga atcggggatc gtgccggatt tcgaagtcgg
     2761 ggaggagttt catgaagagc ccaaaaccta ttacgaactc aaaagtcaac cgctgaagag
     2821 cagcagttcc gccgagcatc ctggggcctc caagcctccg ataagctcct ccagtatgac
     2881 atcacgcatc ttgctacgcc agcaactcat gcgtgagcag atgcaggagc aggagcgcag
     2941 ggagcagcag cagaagctgc aggcggccca gttcatgcaa cagagagtgc ccgtgagtca
     3001 gacaccagcc ataaacgtca gtgtgcccac cacccttccc tctgccacgc aggtgccgat
     3061 ggaagtcctt aaggtgcaga cccacctcga aaaccccacc aagtaccaca tacagcaagc
     3121 ccaacggcag caggtaaagc agtacctttc taccacttta gcaaataaac atgccaacca
     3181 agtcctgagc ttgccatgtc caaaccagcc tggcgatcat gtcatgccac cggtgccggg
     3241 gagcagcgca cccaacagcc ccatggctat gcttacgctt aactccaact gtgaaaaaga
     3301 gggattttat aagtttgaag agcaaaacag ggcagagagc gagtgcccag gcatgaacac
     3361 acattcacga gcgtcctgta tgcagatgga tgatgtaatc gatgacatca ttagcctaga
     3421 atcaagttat aatgaggaaa tcttgggctt gatggatcct gctttgcaaa tggcaaatac
     3481 gttgcctgtc tcgggaaact tgattgatct ttatggaaac caaggtctgc ccccaccagg
     3541 cctcaccatc agcaactcct gtccagccaa ccttcccaac ataaaaaggg agctcacaga
     3601 gtctgaagca agagcactgg ccaaagagag gcagaaaaag gacaatcaca acctgattga
     3661 acgaagaaga agatttaaca taaatgaccg cattaaagaa ctaggtactt tgattcccaa
     3721 gtcaaatgat ccagacatgc gctggaacaa gggaaccatc ttaaaagcat ccgtggacta
     3781 tatccgaaag ttgcaacgag aacagcaacg cgcaaaagaa cttgaaaacc gacagaagaa
     3841 actggagcac gccaaccggc atttgttgct cagaatacag gaacttgaaa tgcaggctcg
     3901 agctcatgga ctttccctta ttccatccac gggtctctgc tctccagatt tggtgaatcg
     3961 gatcatcaag caagaacccg ttcttgagaa ctgcagccaa gacctccttc agcatcatgc
     4021 agacctaacc tgtacaacaa ctctcgatct cacggatggc accatcacct tcaacaacaa
     4081 cctcggaact gggactgagg ccaaccaagc ctatagtgtc cccacaaaaa tgggatccaa
     4141 actggaagac atcctgatgg acgacaccct ttctcccgtc ggtgtcactg atccactcct
     4201 ttcctcagtg tcccccggag cttccaaaac aagcagccgg aggagcagta tgagcatgga
     4261 agagacggag cacacttgtt agtcgcgaat cgatcgccca ccccaacttg tttattgcag
     4321 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4381 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     4441 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     4501 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     4561 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     4621 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4681 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4741 tgcggcgagc ggtatcagct cactcaaagg acggccgcac atgtaaggaa aattttaggg
     4801 atgttaaaga aaaaaataac acaaaacaaa atataaaaaa aatctaacct caagtcaagg
     4861 cttttctatg gaataaggaa tggacagcag ggggctgttt catatactga tgacctcttt
     4921 atagccaacc tttgttcatg gcagccagca tatgggcata tgttgccaaa ctctaaacca
     4981 aatactcatt ctgatgtttt aaatgatttg ccctcccata tgtccttccg agtgagagac
     5041 acaaaaaatt ccaacacact attgcaatga aaataaattt cctttattag ccagaagtca
     5101 gatgctcaag gggcttcatg atgtccccat aatttttggc agagggaaaa agatctcagt
     5161 ggtatttgtg agccagggca ttggccacac cagtcaccac cttctgatag gcagcctgca
     5221 cctgaggagt gaattcacgc gttttagcct ggcagcatat cgagatcgaa gtcatcgaga
     5281 gcgtcagcag ggagcatatc cagatcaaaa tcatccaggg catcggcggg cagcatgtcc
     5341 aggtcgaagt cgtccagggc gtcggtgggt ccgccgctct cgcacttcag ctgcttctcg
     5401 aggccgcaga tgatcagttc caggccgaac aggaaggcgg gctcggcgcc ctgtctgtcg
     5461 aacagctcga tggcctgctt cagcaggggg ggcatgctgt cggtggtggg tgtctctctt
     5521 tcctctttgg cgacctggtg ttcctgttct tccagcacgc agcccagggt gaagtggccc
     5581 acggcgctca gggcgtacag ggcgttttcc aggctgaagc cctgctggca caggaaggcc
     5641 agctggtttt ccagtgtctc gtactgcttc tcggtgggtc tggtgcccag gtgcactttg
     5701 gcgccgtccc ggtggctcag cagggcgcat ctgtagctct tggcgttgtt ccgcaggaag
     5761 tcctgccagc tctcgccttc cagagggcag ctgtgggtgt ggtgccggtc cagcatctcg
     5821 atgggcaggg cgtccagcag ggcccgcttg ttcttcacgt gccagtacag ggtgggctgt
     5881 tccacgccca gtttctgggc cagcttccgg gtggtcaggc cctcgatgcc cacgccgttc
     5941 agcagttcca gggcgctgtt gatcactttg ctcttgtcca gccggctcat gtcgacattc
     6001 tttgccaaaa tgatgagaca gcacaataac cagcacgttg cccaggagct gtaggaaaaa
     6061 gaagaaggca tgaacatggt tagcagaggc tctagagccg ccggtcacac gccagaagcc
     6121 gaaccccgcc ctgccccgtc ccccccgaag gcagccgtcc ccccgcggac agccccgagg
     6181 ctggagaggg agaaggggac ggcggcgcgg cgacgcacga aggccctccc cgcccatttc
     6241 cttcctgccg gcgccgcacc gcttcgcccc gcgcccgcta gagggggtgc ggcggcgcct
     6301 cccagatttc ggctccgcac agatttggga caaaggaagt ccctgcgccc tctcgcacga
     6361 ttaccataaa aggcaatggc tgcggctcgc cgcgcctcga cagccgccgg cgctccgggg
     6421 gccgccgcgc ccctcccccg agccctcccc ggcccgaggc ggccccgccc cgcccggcac
     6481 ccccacctgc cgccaccccc cgcccggcac ggcgagcccc gcgccacgcc ccgtacggag
     6541 ccccgcaccc gaagccgggc cgtgctcagc aactcgggga ggggggtgca gggggggttg
     6601 cagcccgacc gacgcgccca caccccctgc tcaccccccc acgcacacac cccgcacgca
     6661 gcctttgttc ccctcgcagc cccccccgca ccgcggggca ccgcccccgg ccgcgctccc
     6721 ctcgcgcaca ctgcggagcg cacaaagccc cgcgccgcgc ccgcagcgct cacagccgcc
     6781 gggcagcgcg gagccgcacg cggcgctccc cacgcacaca cacacgcacg caccccccga
     6841 gccgctcccc ccgcacaaag ggccctcccg gagcccctca aggctttcac gcagccacag
     6901 aaaagaaaca agccgtcatt aaaccaagcg ctaattacag cccggaggag aagggccgtc
     6961 ccgcccgctc acctgtggga gtaacgcggt cagtcagagc cggggcgggc ggcgcgaggc
     7021 ggcggcggag cggggcacgg ggcgaaggca gcgcgcagcg actcccgccc gccgcgcgct
     7081 tcgcttttta tagggccgcc gccgccgccg cctcgccata aaaggaaact ttcggagcgc
     7141 gccgctctga ttggctgccg ccgcaccycc cccccccgcc cccccccccc cccccccccc
     7201 cccccccccc cctggcgcgc gccccccccc cccccccgcc cccatcgctg cacaaaataa
     7261 ttaaaaaata aataaataca aaattggggg tggggagggg ggggagatgg ggagagtgaa
     7321 gcagaacgtg gggctcacct cgaccatggt aatagcgatg actaatacgt agatgtactg
     7381 ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc
     7441 gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg
     7501 ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac
     7561 tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg
     7621 cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga
     7681 ccccgtaatt gattactatt aataactagt caataatcaa tgtcgagacc catagagccc
     7741 accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc
     7801 accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt
     7861 tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg
     7921 caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg ggtttataac
     7981 ttcgtataat gtatgctata cgaagttatg gtaccttact cgtccatgcc gccggtggag
     8041 tggcggccct cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg
     8101 atgtccaact tgatgttgac gttgtaggcg ccgggcagct gcacgggctt cttggccttg
     8161 taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg
     8221 atctcgccct tcagggcgcc gtcctcgggg tacatccgct cggaggaggc ctcccagccc
     8281 atggtcttct tctgcattac ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc
     8341 ttgtagatga actcgccgtc ctgcagggag gagtcctggg tcacggtcac cacgccgccg
     8401 tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcaagtag
     8461 tcggggatgt cggcggggtg cttcacgtag gccttggagc cgtacatgaa ctgaggggac
     8521 aggatgtccc aggcgaaggg cagggggcca cccttggtca ccttcagctt ggcggtctgg
     8581 gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg
     8641 gagccctcca tgtgcacctt gaagcgcatg aactccttga tgatggccat gttatcctcc
     8701 tcgcccttgc tcaccatggt ggcgaccggt agcgctagcg taggcgccgg tcacagcttg
     8761 gatctgtaac ggcgcagaac agaaaacgaa acaaagacgt agagttgagc aagcagggtc
     8821 aggcaaagcg tggagagccg gctgagtcta ggtaggctcc aagggagcgc cggacaaagg
     8881 cccggtctcg acctgagctt taaacttacc tagacggcgg acgcagttca ggaggcacca
     8941 caggcgggag gcggcagaac gcgactcaac cggcgtggat ggcggcctca ggtagggcgg
     9001 cgggcgcgtg aaggagagat gcgagcccct cgaagcttca gctgtgttct ggcggcaaac
     9061 ccgttgcgaa aaagaacgtt cacggcgact actgcactta tatacggttc tcccccaccc
     9121 tcgggaaaaa ggcggagcca gtacacgaca tcactttccc agtttacccc gcgccacctt
     9181 ctctaggcac cggttcaatt gccgacccct ccccccaact tctcggggac tgtgggcgat
     9241 gtgcgctctg cccactgacg ggcaccggag cgatcgcaga tcatgcatcc atagagccca
     9301 ccgcatcccc agcatgcctg ctattgtctt cccaatcctc ccccttgctg tcctgcccca
     9361 ccccaccccc cagaatagaa tgacacctac tcagacaatg cgatgcaatt tcctcatttt
     9421 attaggaaag gacagtggga gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc
     9481 aaacaacaga tggctggcaa ctagaaggca caggtttaaa cgggccctct agatcaggca
     9541 ccgggcttgc gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg
     9601 acggtgaagc cgagccgctc gtagaagggg aggttgcggg gcgcggaggt ctccaggaag
     9661 gcgggcaccc cggcgcgctc ggccgcctcc actccgggga gcacgacggc gctgcccaga
     9721 cccttgccct ggtggtcggg cgagacgccg acggtggcca ggaaccacgc gggctccttg
     9781 ggccggtgcg gcgccaggag gccttccatc tgttgctgcg cggccagccg ggaaccgctc
     9841 aactcggcca tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac gctctccggc
     9901 gtggtccaga ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc
     9961 ccgacgcgcg tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg gcggtccgga
    10021 tcgacggtgt ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc
    10081 ctggggacgt cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt catctcgagc
    10141 ctagggccgg gattctcctc cacgtcaccg catgttagaa gacttcctct gccctctccg
    10201 ctgccagatc tctcgaggcc ctgtgggagg aagagaagag gtcagaagct tataacttcg
    10261 tataatgtat gctatacgaa gttattgccc cactgtgggg ccgctgggag aagggtaaga
    10321 gagctcaatc ctcatcttac atagaaggaa gtcagtagat aatatcttac atttaaaagt
    10381 caagaaataa cagtctgacc atactatcta gaaatatgga agttaacagt aaggaagtta
    10441 aaacagttga aagtagtttc gtctagaaag aaagacttgg ggaagaacaa agaaggctaa
    10501 ttttcattat gagctttatt gtaacagaaa agagaccact aactcagcta ggttttccca
    10561 attcctcaac actttggggc acagtgttta gtcagagctg tgatcactct ctgagcacat
    10621 ggattgtatc ttaattttca tctcattgaa aatatgtacc atttgtccaa gtggaaactg
    10681 ggctgaagca tgactgtgac caccaccaaa gaaatggcta gactcgctaa ggtctgctgg
    10741 ggtgacccgt gattgtgttg tgcttgatct tgttgagcat ggctctttta agtcaccata
    10801 cctgcccctg ccaatgcacc aaacaccaac ttaaagggac tttcagcttt tgtaagtcag
    10861 tctcagcctt acttctgaac catttctttg acttctttca tgactctgat cctgtcccag
    10921 ttttaagcct cccagccacc agtcatgctg ttttgctctg ctatttacat tcttctctac
    10981 ctactttgga cttgatggta tgcctactat attagttatt tattgctgtg taacaaacca
    11041 ctctgaaaca tagtggctta caaccacaac cattttattt tattatgaaa ctgtgggtca
    11101 gaatgtatat tggcccattg gcatgattct cctgctattg aaaaaggaag agtatgagta
    11161 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
    11221 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
    11281 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
    11341 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
    11401 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
    11461 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
    11521 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
    11581 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
    11641 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
    11701 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    11761 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    11821 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    11881 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    11941 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    12001 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    12061 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    12121 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    12181 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    12241 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    12301 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    12361 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    12421 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    12481 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    12541 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    12601 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    12661 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    12721 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    12781 gcaacgcggc ctttttacgg ttcctggcga attcgccctt agagcagagc caggaacccc
    12841 tgtagggaag gggcaggaga gccaggggca tgagatggtg gacgaggaag ggggacaggg
    12901 aagcctgagc gcctctcctg ggcttgccaa ggactcaaac ccagaagccc agagcagggc
    12961 cttagggaag cgggacggct ctgttggaga gtccagtatt gaattagtga tagatacaca
    13021 gaaaaaaagc aaattgaata ggtgaatgga taaacaaact gtggaacatc cagacaatga
    13081 aatattcacc aataaaaaga aatgagctat caagccacat tggctatgcc cttcacattt
    13141 aaggaggaac cttaaatgca tattgccaaa ttttaaaagc tagtctgaaa atgctacaga
    13201 ctgtatgatt ccaattatat gacattttag aaaaggcaaa actatagaga tcagtggttg
    13261 ccagcggctc atggaggcag gatgaattgg tggaacacag ggatgctttc agggtaatga
    13321 aaatatcttg tgtgatcctg taataatgga tacgtggcat aatgcatttg ttaaaacctg
    13381 taaactagcc aggtatagtg gcttttttgc acctatagtc tcagctactt ggtaggctga
    13441 gacaggaaga tcacttgagc ccaggacttc aagaccagcc tgggtaatat agtaagaccc
    13501 catctctatt ttttaaagaa attaagaaag aaaagaaacc catcaactgt ataacacaaa
    13561 gaatcgtaat gtaaactaca gtcctgagta atgaaacatt tttaaagaaa taaataaatt
    13621 caaggcaatt attaacttca ggaaaaacag taaagtaaac ataagtatac aaatcaaaca
    13681 tggctcagtt gtgaaaatat ttacaaggtc actttaagta acattgaatg ttgatataat
    13741 cccaaaatat atttatgttg gaaggatgag gagatttaat taagggaggt ggtccctgca
    13801 gttacgccaa tgataacccc cgccagaaaa atcttagtag ccttcccttt ttgttttccg
    13861 tgccccaact cggcggattg actcggcccc ttccggaaac acccgaatca acttctagtc
    13921 aaattattgt tcacgccgca atgacccacc cctggcccgc gtctgtggaa ctgacccctg
    13981 gtgtacagga gagttcgctg ctgaaagtgg tcccaaaggg gtactagttt ttaagctccc
    14041 aactccccct cccccagcgt ctggaggatt ccacaccctc gcaccgcagg ggcgaggaag
    14101 tgggcggagt ccggttttgg cgccagccgc tgaggctgcc aagcagaaaa gccaccgctg
    14161 aggagactcc ggtcactgtc ctcgccccgc ctcccccttc cctccccttg gggaccaccg
    14221 ggcgccacgc cgcgaacggt aagtgccgcg gtcgtcggcg cctccgccct ccccctaggg
    14281 ccccaattcc cagcgggcgc ggcgcgcggc ccctcccccc gccgggcgcg cgcccgctgc
    14341 cccgcccttc gtggccgccc ggcgtgggcg gtgccacccc tccccccggc ggccccgcgc
    14401 gcagctcccg gctccctccc ccttcggatg tggcttgagc tgtaggcgcg gagggtac
//
