LOCUS Exported 3310 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Multisite gateway vector for 5' tagging with lamp1. ACCESSION . VERSION . KEYWORDS pME-lamp1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3310) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3310) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3310 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..100 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(867..966) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(983..1002) /label=T7 /note="T7 promoter, forward primer" promoter complement(984..1002) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1007..1023) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1007..1023) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1125..1144) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1136..1945 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1203..1222) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2092..2680 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2581..2600 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2834..2851 /label=L4440 /note="L4440 vector, forward primer" terminator 3010..3037 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3129..3215 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3264..3286 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3278..3295 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3279..3295 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct gccaccatga tctctatcat 121 ccccaaacaa aacagacccg tcggggcaat ttttagcctg ctgttagctc taaccataca 181 ccagactttg acctccaatg ttccgaccac tgcggccacc cctgaaacca gctctcctcc 241 ggcctctcct gtcacccccg gacccccaga gcggggcaac tacaatgtca ccaatgccaa 301 tgatacagtc tgtctcctgg cacgcatggc cctccaactc aacataagct tcaactcaac 361 ctctcagggc aagcttgtcc aggaggtctt gaacttgcat ccaaacctcg ttaaattctc 421 tggatcctgt gagttggacc gtgcatctct gattctcaca gaggacaaca ttactctgac 481 tttcagcttc tctttaaact ccacatcaaa caagtatcat cttagtggtt tggagctgtc 541 agcttcattg cctgatatgg ctaagggtct tgtggtcaga aacaccacac tgaactactt 601 tgtgggaacg ctgggccact cttacatgtg tcagaaggaa aagaccttga gcgtcaccca 661 aaatttctct ctcaacacct tccaacttca agtgcaacct ttcggtgtca agggagattt 721 tggagcagct gaggagtgcg agttggatga ggacgacatg ttgatcccca tcgttgtggg 781 cgctgcattg gctgttctag tgctcatagt gatcctggcg tacctgatcg gcagaaacag 841 gagtcacgcc ggctaccaga ccatccaccc agctttcttg tacaaagttg gcattataag 901 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat 961 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc 1021 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat 1081 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag 1141 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata 1201 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta 1261 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga 1321 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat 1381 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa 1441 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct 1501 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga 1561 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag 1621 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa 1681 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct 1741 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga 1801 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca 1861 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca 1921 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag 1981 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta 2041 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 2101 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 2161 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 2221 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc 2281 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 2341 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 2401 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 2461 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 2521 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 2581 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 2641 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 2701 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 2761 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg 2821 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc 2881 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 2941 gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt 3001 agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt 3061 tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct 3121 cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag 3181 gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc 3241 gttaacgcta gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa 3301 gctcgggccc //