LOCUS Exported 3166 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with ARF6. ACCESSION . VERSION . KEYWORDS p3E-ARF6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3166) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3166) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3166 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(1..125) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(652..747) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(784..803) /label=T7 /note="T7 promoter, forward primer" promoter complement(785..803) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(808..824) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(808..824) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(926..945) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 937..1746 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1004..1023) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 1893..2481 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2382..2401 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2635..2652 /label=L4440 /note="L4440 vector, forward primer" terminator 2811..2838 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 2930..3016 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3065..3087 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3079..3096 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3080..3096 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc 61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa 121 gtggctggga agatgctttc caagatcttc ggcaacaagg agatgagaat attgatgctc 181 ggacttgacg ccgccggaaa gacaacgatt ctgtacaaac tcaaactggg ccagtcggtt 241 acgaccatcc cgacagtggg cttcaatgta gagactgtca cgtataaaaa cgtcaagttt 301 aacgtttggg acgtcggcgg tcaggataag atccgtcctc tatggcggca ctactacacc 361 ggcacgcagg gcctgatttt cgtagtggac tgtgcggatc gtgaccgcat cgatgaggct 421 cggcaggagc tgcaccgcat tatcaacgat cgtgagatgc gcgacgccat tatcctgatc 481 ttcgccaaca aacaagacct tccagacgcc atgaagccgc atgagataca ggagaagctg 541 ggcctgacgc gcatccgaga tcgcaactgg tacgttcagc cgtcttgcgc tacaaccggc 601 gacggactct acgaagggct cacctggctc acgtccaact acaaatccta acaactttat 661 tatacaaagt tggcattata aaaaagcatt gcttatcaat ttgttgcaac gaacaggtca 721 ctatcagtca aaataaaatc attatttgga gctccatggt agcgttaacg cggccgcgat 781 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg 841 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa 901 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg 961 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc 1021 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca 1081 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc 1141 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact 1201 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta 1261 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg 1321 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct 1381 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt 1441 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg 1501 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa 1561 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc 1621 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa 1681 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt 1741 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg 1801 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg 1861 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 1921 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 1981 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 2041 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 2101 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 2161 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 2221 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 2281 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 2341 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 2401 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 2461 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 2521 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 2581 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 2641 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg 2701 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 2761 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat 2821 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc 2881 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac 2941 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag 3001 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg 3061 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc ctgcagctct 3121 agagctcgaa ttctacaggt cactaatacc atctaagtag ttggtt //