LOCUS Exported 3292 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with rab14l. ACCESSION . VERSION . KEYWORDS p3E-rab14l SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3292) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3292) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3292 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(1..25) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(678..773) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(810..829) /label=T7 /note="T7 promoter, forward primer" promoter complement(811..829) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(834..850) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(834..850) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(952..971) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 963..1772 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1030..1049) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 1919..2507 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2408..2427 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2661..2678 /label=L4440 /note="L4440 vector, forward primer" terminator 2837..2864 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 2956..3042 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3091..3113 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3105..3122 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3106..3122 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 gttcaacttt cttgtacaaa gtggtggcca ctgcacctta caactactcc tacatcttca 61 agtacatcat cattggtgat atgggtgtag ggaagtcctg cctgctgcat cagttcacag 121 agaagaaatt catggcggac cgtccgcaca ctatcggcgt ggagttcggc accaggatca 181 tggaggtgag cggacagaag atcaagctgc agatctggga cacggcgggt caggagcgct 241 tcagagccgt cacacgcagc tactatagag gagccgctgg agcactgatg gtctacgaca 301 tcaccaggag aagcacatac aaccacctca gcagctggct gacggacgcc agaaacctca 361 ccaaccccaa cactgtcatc atactgatag gaaataaagc cgatctggag gcgcagcggg 421 acgtgacgta cgaggaggcc aagcagtttg cggaggagaa cggtctgctg ttcctggagg 481 ccagcgctaa aaccggagag aacgtagagg acgctttcct ggaagcagcc aagaagatct 541 accagaacat tcaggacggc agtctggatc tgaacgcggc agaatccggc gtccagcaca 601 aaccctcagc gccgcaggga ggaagactga cgagtgaaac acagccgcag cgggaggggt 661 gtggctgcta attcatccaa ctttattata caaagttggc attataaaaa agcattgctt 721 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttggagctc 781 catggtagcg ttaacgcggc cgcgatatcc cctatagtga gtcgtattac atggtcatag 841 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata 901 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg 961 ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg 1021 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc 1081 gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg 1141 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc 1201 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc 1261 ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg 1321 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta 1381 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg 1441 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa 1501 tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg 1561 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa 1621 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt 1681 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc 1741 agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact 1801 ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg 1861 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 1921 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 1981 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 2041 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 2101 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 2161 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 2221 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 2281 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 2341 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 2401 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 2461 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 2521 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 2581 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 2641 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 2701 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 2761 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag 2821 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct 2881 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa 2941 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa 3001 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta 3061 ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca 3121 gtcttaagct cgggccctgc agctctagag ctcgaattct acaggtcact aataccatct 3181 aagtagttgg ttcatagtga ctgcatatgt tgtgttttac agtattatgt agtctgtttt 3241 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tc //