LOCUS Exported 3277 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with rab15. ACCESSION . VERSION . KEYWORDS p3E-rab15 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3277) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3277) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3277 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(1..125) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(763..858) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(895..914) /label=T7 /note="T7 promoter, forward primer" promoter complement(896..914) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(919..935) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(919..935) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1037..1056) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1048..1857 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1115..1134) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2004..2592 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2493..2512 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2746..2763 /label=L4440 /note="L4440 vector, forward primer" terminator 2922..2949 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3041..3127 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3176..3198 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3190..3207 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3191..3207 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc 61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa 121 gtggtggcca aacagtacga tgtgctgttc agactcctgc tgctcgggga ctccggtgtc 181 ggcaaaacct gtttattatg cagattcacg gacaatgaat ttcatccctc tcacatttcc 241 accatcggtg tcgatttcaa aatgaagacc ttagagatag atggcattaa agtacggata 301 cagatatggg acacagcggg tcaggagaga tatcagacca tcactaagca gtactacaga 361 agagcacagg gtatatttct ggtgtatgac atcacaagcg agcgctcgtt ccagcacatc 421 atgaagtggg ccagtgatgt agatgagtac gcccctgaaa aggtgcagaa gatacttgtt 481 gggaacaaat ctgatgaaca agagaagaga caagtagcta cagaacaggg agataagctc 541 gccaaagctt atggaatgga cttttttgag acaagtgcct ttaccaacca caacattaca 601 gagtctttca cccgactggc tgaattagta ttacaagcca acaagaagga tcttgatttg 661 ctgggaggat cattgaatga cgagttgaat ctggctgatt tggaggagca ggaaggtttg 721 tgtaatgtta gcgacgacac tcgaaagtcc tgctggtgct gacaacttta ttatacaaag 781 ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc 841 aaaataaaat cattatttgg agctccatgg tagcgttaac gcggccgcga tatcccctat 901 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat 961 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct 1021 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg 1081 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc 1141 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt 1201 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac 1261 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat 1321 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat 1381 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg 1441 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa 1501 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg 1561 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc 1621 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt 1681 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg 1741 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt 1801 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca 1861 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca 1921 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc 1981 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 2041 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 2101 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 2161 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 2221 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 2281 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 2341 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 2401 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 2461 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 2521 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 2581 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 2641 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 2701 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 2761 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 2821 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 2881 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga 2941 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc 3001 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag 3061 cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt 3121 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag 3181 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cctgcagctc tagagctcga 3241 attctacagg tcactaatac catctaagta gttggtt //