LOCUS Exported 3310 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with rab20. ACCESSION . VERSION . KEYWORDS p3E-rab20 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3310) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3310) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3310 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(1..25) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(696..791) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(828..847) /label=T7 /note="T7 promoter, forward primer" promoter complement(829..847) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(852..868) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(852..868) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(970..989) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 981..1790 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1048..1067) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 1937..2525 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2426..2445 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2679..2696 /label=L4440 /note="L4440 vector, forward primer" terminator 2855..2882 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 2974..3060 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3109..3131 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3123..3140 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3124..3140 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 gttcaacttt cttgtacaaa gtggtgaacg ttggaaagac ttctctcctc cacagataca 61 cggagaggaa gtttaaggac acgataagca ccgtcggagg ggcgtttttc ctcaaacagt 121 ggggtcccta caacatttcc atatgggata ctgcaggtcg agagcagttt cacggtctgg 181 gctccatgta ctgccgtggc gcagctgctg tcatcctaac atatgatgtc accaactggc 241 agagcctggc ggagctggag aaccgcttcc tgtccttgac tgacactgcc aacgcagact 301 gcatcttcgc ccttgtgggg aacaaagcgg acctgaccga cccgttcgct caagagcctg 361 acagcgagag tgggagcgac ccgcagcctg tagcctcctg cccgactcct cctgccagcc 421 ctgtccagca caaacaggtg agcagagagg acgctgtggc tctgtacgcc cgcgttctcc 481 gatacaaagg cctggacgag aagacctgcc tgcccgccga gaggatgtgc ttcgagacca 541 gcgctaaaac aggttacaac gtggacgctt tgttcgagac cttatttgat ttagtactgc 601 catccattct gaagaagagg agcgagggtg aagccactgt agatctggag acgccactag 661 aggtgagaag gactaaagct ggctgctgtg catagcaact ttattataca aagttggcat 721 tataaaaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa 781 aatcattatt tggagctcca tggtagcgtt aacgcggccg cgatatcccc tatagtgagt 841 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat 901 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa 961 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa 1021 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat 1081 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac 1141 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga 1201 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt 1261 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt 1321 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg 1381 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa 1441 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg 1501 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc 1561 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg 1621 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc 1681 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc 1741 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg 1801 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat 1861 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa 1921 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 1981 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 2041 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 2101 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 2161 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 2221 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 2281 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 2341 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 2401 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 2461 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 2521 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 2581 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 2641 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 2701 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 2761 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc 2821 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt 2881 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg 2941 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac 3001 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg 3061 atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac 3121 gttgtaaaac gacggccagt cttaagctcg ggccctgcag ctctagagct cgaattctac 3181 aggtcactaa taccatctaa gtagttggtt catagtgact gcatatgttg tgttttacag 3241 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt 3301 tacgtttctc //