LOCUS Exported 3301 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with rab3aa. ACCESSION . VERSION . KEYWORDS p3E-rab3aa SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3301) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3301) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3301 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(1..125) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(787..882) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(919..938) /label=T7 /note="T7 promoter, forward primer" promoter complement(920..938) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(943..959) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(943..959) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1061..1080) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1072..1881 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1139..1158) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2028..2616 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2517..2536 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2770..2787 /label=L4440 /note="L4440 vector, forward primer" terminator 2946..2973 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3065..3151 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3200..3222 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3214..3231 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3215..3231 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc 61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa 121 gtggtggctt cagcgaatga tgctcggtat ggacaaaaag actctgcaga acagaacttt 181 gactacatgt ttaaggtcct gatcattggt aacagcagtg ttggcaaaac tagtttcctg 241 ttccgttatg ctgatgactc cttcacacct gcttttgtca gcactgtcgg catagacttc 301 aaagtcaaga ccatctatag gaacgacaag aggataaagc tccaaatatg ggacactgct 361 gggcaggaga ggtacaggac catcaccaca gcttactatc gcggagcaat gggcttcatt 421 cttatgtatg acatcaccaa cgaggattct tttaatgccg ttcaggactg gtccacacag 481 attaagacgt attcatggga caacgctcaa gtgctgctgg tgggaaataa gtgtgatatg 541 gaagatgaga gagttgttgc tggacagaga ggcagacaat tgtctgaaca gctaggcttt 601 gagttttttg aagccagtgc caaggacaat ataaacgtca agcagacatt tgagcgtctg 661 gtggatgtaa tctgtgagag aatgtcagag aacctggagt cagcagatcc atccatgact 721 ggaaacaagc tgggtcccga gctctcagag caaccgcagc gctcacacaa ggactgcgct 781 tgctaacaac tttattatac aaagttggca ttataaaaaa gcattgctta tcaatttgtt 841 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttggagctcc atggtagcgt 901 taacgcggcc gcgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg 961 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca 1021 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat 1081 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg 1141 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg 1201 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt 1261 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag 1321 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca 1381 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca 1441 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc 1501 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat 1561 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt 1621 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt 1681 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga 1741 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa 1801 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg 1861 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt 1921 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg 1981 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 2041 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 2101 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 2161 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta 2221 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 2281 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 2341 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 2401 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 2461 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 2521 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 2581 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 2641 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 2701 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 2761 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct 2821 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 2881 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa 2941 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg 3001 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg 3061 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc 3121 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta 3181 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc 3241 gggccctgca gctctagagc tcgaattcta caggtcacta ataccatcta agtagttggt 3301 t //