LOCUS Exported 3274 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with rab6ba. ACCESSION . VERSION . KEYWORDS p3E-rab6ba SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3274) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3274) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3274 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(1..125) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(760..855) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(892..911) /label=T7 /note="T7 promoter, forward primer" promoter complement(893..911) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(916..932) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(916..932) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1034..1053) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1045..1854 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1112..1131) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2001..2589 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2490..2509 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2743..2760 /label=L4440 /note="L4440 vector, forward primer" terminator 2919..2946 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3038..3124 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3173..3195 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3187..3204 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3188..3204 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc 61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa 121 gtggtgtctg tgggaaatga ctttggaaac cctttaagga aattcaaact cgtctttctt 181 ggagagcaaa gcgtggggaa gacttccctg atcacacgct tcatgtatga cagctttgac 241 aacacatatc aggctacaat tggaatcgac ttcctgtcaa aaaccatgta tttggaggac 301 agaacggttc ggttgcagtt gtgggacacg gcaggccagg aacgttttag gagcctcatc 361 ccgagctata taagagattc cactgtagct gtggtggtgt atgacatcac aaatgtgaat 421 tcgtttcaac aaacttccaa atggatcgat gacgtgagaa cggagcgagg aagtgatgtc 481 atcatcatgc tggtgggcaa taaaacagat ctggcagata agaggcagat cacgattgag 541 gagggagagc agagggccaa agagctgagt gtgatgttta tcgagaccag cgccaagacg 601 ggttacaacg tcaagcagct gtttcgacgt gtggcagctg cccttccagg aatggagagc 661 atgcaggaga caagcaaaga aggcatgatc gacattaaat tggacaaacc acaggagcct 721 ccaaccactg aaggcggatg ttcctgttaa taactcacac aactttatta tacaaagttg 781 gcattataaa aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa 841 ataaaatcat tatttggagc tccatggtag cgttaacgcg gccgcgatat cccctatagt 901 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc 961 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac 1021 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga 1081 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg 1141 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg 1201 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg 1261 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca 1321 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct 1381 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt 1441 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca 1501 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct 1561 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc 1621 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt 1681 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac 1741 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat 1801 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa 1861 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc 1921 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta 1981 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 2041 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 2101 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 2161 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 2221 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 2281 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 2341 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 2401 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 2461 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 2521 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 2581 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 2641 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 2701 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 2761 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 2821 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 2881 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg 2941 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg 3001 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt 3061 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta 3121 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca 3181 cgacgttgta aaacgacggc cagtcttaag ctcgggccct gcagctctag agctcgaatt 3241 ctacaggtca ctaataccat ctaagtagtt ggtt //