LOCUS Exported 3265 bp ds-DNA circular SYN 19-OCT-2023 DEFINITION Multisite gateway vector for 3' tagging with rab6bb. ACCESSION . VERSION . KEYWORDS p3E-rab6bb SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3265) AUTHORS Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim YW, Parton RG TITLE In vivo cell biological screening identifies an endocytic capture mechanism for T-tubule formation. JOURNAL Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w. PUBMED 32709891 REFERENCE 2 (bases 1 to 3265) AUTHORS . TITLE Direct Submission JOURNAL Exported Oct 19, 2023 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3265 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(1..125) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(751..846) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(883..902) /label=T7 /note="T7 promoter, forward primer" promoter complement(884..902) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(907..923) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(907..923) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1025..1044) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1036..1845 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1103..1122) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 1992..2580 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2481..2500 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2734..2751 /label=L4440 /note="L4440 vector, forward primer" terminator 2910..2937 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3029..3115 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3164..3186 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3178..3195 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3179..3195 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc 61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa 121 gtggtgtcca tgggaagtga ctttgggaac cctttacgga aattcaaact tgttttttta 181 ggggagcaaa gcgttgggaa gacatcgctg ataacaaggt tcatgtatga cagttttgac 241 aacacttatc aggcaactat tggcattgac tttttatcaa aaaccatgta tctggaggac 301 cgaactgtga ggttacagct atgggacact gcagggcagg agcgttttag gagtcttatc 361 ccgagctaca ttagagactc cacagtggct gtggtggtgt atgatatcac aaatatcaat 421 tcattccagc taacctctaa atggattgat gatgtcagaa cagagagggg aagtgatgtc 481 attatcatgc tggtgggcaa taaaacagat ctagaggaaa agaggcaaat cactatagag 541 gagggggagc agcgggccaa agagttgaat gtgatgttta tcgagactag tgcaaagacc 601 ggcagtaatg ttaaacagtt gtttcggcga gtggccgctg ctttgcctgg tatggagagc 661 ttggatgata ataacaaaga aggaatgatt gacattaagc tggacaaaca gccggatcct 721 ccggccactg aaagcggctg ctcctgttag caactttatt atacaaagtt ggcattataa 781 aaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca 841 ttatttggag ctccatggta gcgttaacgc ggccgcgata tcccctatag tgagtcgtat 901 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac 961 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt 1021 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc 1081 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt 1141 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc 1201 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa 1261 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc 1321 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt 1381 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt 1441 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat 1501 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa 1561 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt 1621 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt 1681 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt 1741 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat 1801 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat 1861 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca 1921 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc 1981 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 2041 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 2101 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 2161 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 2221 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 2281 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 2341 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 2401 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 2461 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 2521 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 2581 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 2641 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 2701 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 2761 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 2821 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc 2881 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct 2941 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct 3001 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca 3061 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc 3121 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt 3181 aaaacgacgg ccagtcttaa gctcgggccc tgcagctcta gagctcgaat tctacaggtc 3241 actaatacca tctaagtagt tggtt //