LOCUS Exported 3280 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with constitutively active rab4a. ACCESSION . VERSION . KEYWORDS p3E-rab4a-CA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3280) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3280) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3280 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(1..125) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(766..861) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(898..917) /label=T7 /note="T7 promoter, forward primer" promoter complement(899..917) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(922..938) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(922..938) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1040..1059) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1051..1860 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1118..1137) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2007..2595 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2496..2515 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2749..2766 /label=L4440 /note="L4440 vector, forward primer" terminator 2925..2952 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3044..3130 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3179..3201 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3193..3210 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3194..3210 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc 61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa 121 gtggtgtcag agacatacga tttcttgttt aagtttcttg tgattgggaa tgctggaaca 181 ggaaaatcct gtcttcttca tcagttcata gaaaaaagat tcaaagatga ctcaaatcac 241 actattgggg ttgaatttgg ttcgaagata atcagcgtgg tcaataagtt tgtaaagctc 301 cagatctggg acacagcagg actggagcgt ttcaggtctg taactaggag ttattacaga 361 ggcgcagcag gggctcttct agtctatgac atcactagtc gagaaacata caacgctcta 421 accaactggc tgacagatgc ccggatgttg gccagtcaga acatagtgat catactatgt 481 ggaaacaaga aggatctgga tgctgaccgc gaggtgacct ttctggaggc ttcacgcttc 541 gctcaggaga atgagcttat gtttttggag accagtgctc tgacagggga gaatgtggag 601 gaagctttcg tccagtgtgc tcgaaaaatc cttaacaaaa ttgaatcagg agagctggac 661 ccagagcgga tgggttcagg aatccagtat ggagacgcgg ctcttcgaca gctgcgttca 721 cccagacggg cacaagccga gagcatccag gagtgtggct gttaacaact ttattataca 781 aagttggcat tataaaaaag cattgcttat caatttgttg caacgaacag gtcactatca 841 gtcaaaataa aatcattatt tggagctcca tggtagcgtt aacgcggccg cgatatcccc 901 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa 961 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct 1021 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg 1081 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat 1141 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg 1201 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta 1261 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat 1321 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa 1381 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat 1441 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg 1501 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc 1561 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca 1621 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata 1681 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta 1741 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt 1801 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa 1861 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc 1921 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac 1981 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 2041 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 2101 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 2161 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 2221 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 2281 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 2341 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 2401 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 2461 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 2521 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 2581 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 2641 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 2701 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 2761 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 2821 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 2881 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca 2941 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc 3001 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga 3061 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc 3121 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc 3181 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccctgcag ctctagagct 3241 cgaattctac aggtcactaa taccatctaa gtagttggtt //