LOCUS Exported 3223 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Multisite gateway vector for 3' tagging with Rab31. ACCESSION . VERSION . KEYWORDS p3E-Rab31 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3223) TITLE Parton Lab Plasmids for in vivo cell biology in the zebrafish REFERENCE 2 (bases 1 to 3223) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3223 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(1..125) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(709..804) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(841..860) /label=T7 /note="T7 promoter, forward primer" promoter complement(842..860) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(865..881) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(865..881) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(983..1002) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 994..1803 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1061..1080) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 1950..2538 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2439..2458 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2692..2709 /label=L4440 /note="L4440 vector, forward primer" terminator 2868..2895 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 2987..3073 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3122..3144 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3136..3153 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3137..3153 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc 61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa 121 gtggtggcca taagggagct aaaagtttgt ctgctcgggg acactggtgt ggggaaatcg 181 agtatcgtgt gtcggtttgt tcaagatcac tttgaccaca acattagtcc gacaataggt 241 gcatcatttt taactaagac cgtcccctct ggaaatgaac ttcacaaatt tctgatctgg 301 gacactgcag gacaagagcg gtttcattcc ctggctccca tgtattacag aggctcggca 361 gcggctgtca ttgtttatga catcacaaag cttgactcgt tccagacgtt aaagaagtgg 421 gtgaaagagc tgaaggagca cgggccggag gatatagtgg tggccatcgc tggaaataag 481 aacgatctgg gtgacatcag ggaagttcca actaaggagg cgaaagaatt tgcagagtcg 541 attgcagcca tatttatgga gaccagtgcc agaaatgctg taaacataga ggagctgttc 601 caaaaaatca gtcgacagat ccctccactg gagaacactg atgcagacag ccacgactcc 661 ttcaagctga cccgacagcc tcctccagcc accaaacgct gctgttagca actttattat 721 acaaagttgg cattataaaa aagcattgct tatcaatttg ttgcaacgaa caggtcacta 781 tcagtcaaaa taaaatcatt atttggagct ccatggtagc gttaacgcgg ccgcgatatc 841 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct 901 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg 961 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg 1021 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat 1081 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag 1141 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga 1201 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct 1261 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa 1321 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg 1381 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag 1441 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat 1501 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat 1561 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta 1621 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc 1681 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat 1741 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc 1801 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac 1861 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca 1921 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 1981 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 2041 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 2101 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 2161 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 2221 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 2281 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 2341 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 2401 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 2461 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 2521 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 2581 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 2641 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 2701 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 2761 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 2821 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg 2881 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc 2941 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca 3001 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct 3061 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt 3121 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggccctg cagctctaga 3181 gctcgaattc tacaggtcac taataccatc taagtagttg gtt //