LOCUS       Exported                6796 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCAX APP 695
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Young-Pearse TL, Bai J, Chang R, Zheng JB, LoTurco JJ, Selkoe DJ
  TITLE     A critical function for beta-amyloid precursor protein in neuronal 
            migration revealed by in utero RNA interference.
  JOURNAL   J Neurosci. 2007 Dec 26. 27(52):14459-69.
  PUBMED    18160654
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        117..496
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        527..631
                     /gene="bla"
                     /label=AmpR promoter
     CDS             632..1492
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(850..869)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1663..2251
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2152..2171
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2405..2422
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    2490..2624
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2514..2533)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2568..2587
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(2630..2825)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2644..2779
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2698..2717)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    2884..2905
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2920..2950
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2958..2974
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2963..2985
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2982..2998
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2982..2998
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3341..3360
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    3359..3414
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     3460..3479
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(4752..4772)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             5252..5263
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     primer_bind     complement(5636..5655)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5663..6679
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5717..5739)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 cccccccccg cccccatcgc tgcacaaaat aattaaaaaa taaataaata caaaattggg
       61 ggtggggagg ggggggagat ggggagagtg aagcagaacg tggggctcac ctcgaccatg
      121 gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg
      181 tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt
      241 ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat
      301 tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc
      361 aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg
      421 cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta
      481 gtcaataatc aatgtcgacc caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      541 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      601 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      661 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      721 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      781 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      841 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      901 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      961 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     1021 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     1081 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     1141 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     1201 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     1261 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     1321 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     1381 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1441 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     1501 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     1561 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1621 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1681 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1741 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1801 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1861 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1921 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1981 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2041 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2101 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2161 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2221 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2281 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     2341 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     2401 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     2461 cgcgcgttgg ccgattcatt aatgcagcgg atccagacat gataagatac attgatgagt
     2521 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     2581 ctattgcttt atttgtaacc attataagct gcaataaaca agttagcttt ttgcaaaagc
     2641 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct
     2701 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
     2761 gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga
     2821 gatgcggatc cgctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     2881 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     2941 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     3001 tgattacgcc aagcttgggc tgcaggtcga gggatctcca taagagaaga gggacagcta
     3061 tgactgggag tagtcaggag aggaggaaaa atctggctag taaaacatgt aaggaaaatt
     3121 ttagggatgt taaagaaaaa aataacacaa aacaaaatat aaaaaaaatc taacctcaag
     3181 tcaaggcttt tctatggaat aaggaatgga cagcaggggg ctgtttcata tactgatgac
     3241 ctctttatag ccaacctttg ttcatggcag ccagcatatg ggcatatgtt gccaaactct
     3301 aaaccaaata ctcattctga tgttttaaat gatttgccct cccatatgtc cttccgagtg
     3361 agagacacaa aaaattccaa cacactattg caatgaaaat aaatttcctt tattagccag
     3421 aagtcagatg ctcaaggggc ttcatgatgt ccccataatt tttggcagag ggaaaaagat
     3481 ctctcgaggc ggccgcgata tcctagttct gcatctgctc aaagaacttg taggttggat
     3541 tttcgtagcc gttctgctgc atcttggaca ggtggcgctc ctctggggtg acagcggcgt
     3601 caacctccac cacaccatga tgaatggatg tgtactgttt cttcttcagc atcaccaagg
     3661 tgatgacgat cactgtcgct atgacaacac cgcccaccat gagtccaatg attgcacctt
     3721 tgtttgaacc cacatcttct gcaaagaaca ccaatttttg atgatgaact tcatatcctg
     3781 agtcatgtcg gaattctgca tccatcttca cttcagagat ctcctccgtc ttgatatttg
     3841 tcaacccaga acctggtcga gtggtcagtc ctcggtcggc agcagggcgg gcatcaacag
     3901 gctcaacttc gttttctgtg ttggctggca cagagtcagc cccaaaagaa tgccacggct
     3961 ggagatcgtc caggctgaac tctccattca cgggaaggag ctccacggtg gttttcgttt
     4021 cggtcaaaga tggcatgaga gcatcgtttc cgtaactgat ccttggttca ctaatcatgt
     4081 tggccaagac gtcatctgaa tagttttgct ctttctgaag cagctcatca acttcatcct
     4141 gaatctcctc ggccactgca ggcacgttgt agagcaggga gagagactga ttcatgcgct
     4201 cataaatcac acggaggtgt gtcataacct gggaccggat ctgagcggct ttcttgggat
     4261 ccaccatgcg cacatgctcg aaatgcttta gggtgtgctg tctgtccttc tgttctgcgc
     4321 ggacatactt ctttagcata ttgaacacgt gacgaggccg aggaggaaca gcctgcagag
     4381 cggtgatgta gttctccagg gccaggcggc ggcggtcatt gagcatggct tccactctgg
     4441 ccatgtgtgt ctccaccagc tgctgtctct cgttggctgc ttcctgttcc aaagattcca
     4501 ctttctcctg gaaatgctgg ataactgcct tcttatcagc tttaggcaag ttctttgctt
     4561 gacgttctgc ctcttcccat tctctcatga cctgggacat tctctctcgg tgcttggcct
     4621 caagcctctc tttggctttc tggaaatggg catgttcatt ctcatcccca ggtgtctcga
     4681 gatacttgtc aacggcatca ggggtactgg ctgctgttgt aggaactcga accacctctt
     4741 ccacagactc tgtggtggtg gtggtggtgg tggcaatgct ggtggttctc tctgtggctt
     4801 cttcgtaggg ttcctcagcc tcttcctcta cctcatcacc atcctcatcg tcctcgtcat
     4861 catcggcttc ttcttcttcc acctcagcca cttcttcctc ctctgctact tctactactt
     4921 tgtcttcact cccatctgca tagtctgtgt ctgctccgcc ccaccagaca tccgagtcat
     4981 cctcctccgc atcagcagaa tccacattgt cactttcttc agccagtggg caacacacaa
     5041 actctacccc tcggaacttg tcaattccgc agggcagcaa catgccgtag tcatgcaagt
     5101 tggtactctt ctcactgcat gtctctttgg cgacggtgtg ccagtgaaga tgagtttcgc
     5161 aaacatccat cctctcctgg tgtaagaatt tgcacttgtc aggaacgaga agggcatcac
     5221 ttacaaactc accaactaag cagcggtagg gaatcacaaa gtggggatgg gtcttgcact
     5281 gcttgcggcc ccgcttgcac cagttctgga tggtcactgg ttggttggct tctaccacat
     5341 tggtgatctg cagttcaggg tagacttctt ggcaatactg caggatgcct tccttggtat
     5401 caatgcaggt tttggtccct gatggatctg aatcccactt cccattctgg acattcatgt
     5461 gcatgttcag tctgccacag aacatggcaa tctggggttc agccagcagg ccagcattac
     5521 catcagtggg tacctccagc gcccgagccg tccaggcggc cagcaggagc agtgccaaac
     5581 cgggcagcat ggcaagctat cggtacctga attctttgcc aaaatgatga gacagcacaa
     5641 taaccagcac gttgcccagg agctgtagga aaaagaagaa ggcatgaaca tggttagcag
     5701 aggctctaga gccgccggtc acacgccaga agccgaaccc cgccctgccc cgtccccccc
     5761 gaaggcagcc gtccccccgc ggacagcccc gaggctggag agggagaagg ggacggcggc
     5821 gcggcgacgc acgaaggccc tccccgccca tttccttcct gccggcgccg caccgcttcg
     5881 ccccgcgccc gctagagggg gtgcggcggc gcctcccaga tttcggctcc gcacagattt
     5941 gggacaaagg aagtccctgc gccctctcgc acgattacca taaaaggcaa tggctgcggc
     6001 tcgccgcgcc tcgacagccg ccggcgctcc gggggccgcc gcgcccctcc cccgagccct
     6061 ccccggcccg aggcggcccc gccccgcccg gcacccccac ctgccgccac cccccgcccg
     6121 gcacggcgag ccccgcgcca cgccccgtac ggagccccgc acccgaagcc gggccgtgct
     6181 cagcaactcg gggagggggg tgcagggggg gttgcagccc gaccgacgcg cccacacccc
     6241 ctgctcaccc ccccacgcac acaccccgca cgcagccttt gttcccctcg cagccccccc
     6301 cgcaccgcgg ggcaccgccc ccggccgcgc tcccctcgcg cacactgcgg agcgcacaaa
     6361 gccccgcgcc gcgcccgcag cgctcacagc cgccgggcag cgcggagccg cacgcggcgc
     6421 tccccacgca cacacacacg cacgcacccc ccgagccgct ccccccgcac aaagggccct
     6481 cccggagccc ctcaaggctt tcacgcagcc acagaaaaga aacaagccgt cattaaacca
     6541 agcgctaatt acagcccgga ggagaagggc cgtcccgccc gctcacctgt gggagtaacg
     6601 cggtcagtca gagccggggc gggcggcgcg aggcggcggc ggagcggggc acggggcgaa
     6661 ggcagcgcgc agcgactccc gcccgccgcg cgcttcgctt tttatagggc cgccgccgcc
     6721 gccgcctcgc cataaaagga aactttcgga gcgcgccgct ctgattggct gccgccgcac
     6781 ctctccgcct cgcccc
//
