LOCUS Exported 7217 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS p6599 MSCV-IP N-HAonly FOSL1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7217) AUTHORS White EA, Sowa ME, Tan MJ, Jeudy S, Hayes SD, Santha S, Munger K, Harper JW, Howley PM TITLE Systematic identification of interactions between host cell proteins and E7 oncoproteins from diverse human papillomaviruses. JOURNAL Proc Natl Acad Sci U S A. 2012 Jan 9. PUBMED 22232672 REFERENCE 2 (bases 1 to 7217) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7217 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..517 /label=5' LTR /note="5' long terminal repeat from murine embryonic stem cell virus" misc_feature 581..922 /label=MESV Psi /note="packaging signal of murine embryonic stem cell virus" CDS 989..1405 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1335..1357 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1373..1389 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" CDS 1439..1465 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" protein_bind 1481..1505 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(2319..2343) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 2373..2925 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(2517..2534) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 2744..2763 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 2937..3536 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(2937..2956) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 3433..3453 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 3612..4126 /label=3' LTR /note="3' long terminal repeat from murine embryonic stem cell virus" protein_bind 4288..4304 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4312..4342) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4357..4378 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4495..4512) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4666..5254) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4746..4765) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5425..6285) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6048..6067 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6286..6390) /gene="bla" /label=AmpR promoter primer_bind 6458..6476 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(6514..6536) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 6636..6655 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 6849..6871 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 6999..7018 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" ORIGIN 1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc 61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag 121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa 181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt 241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt 301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac 361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa 421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct 481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga 541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt 601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct 661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg 721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc 781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg 841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg 901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac 961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac 1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa 1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg 1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg 1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga 1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc 1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc 1381 agccctcact ccttctctag gcgccggaat tagatctgcc accatgctcg atggaggata 1441 cccatacgat gttccagatt acgctgctcg aggttatcaa acaagtttgt acaaaaaagt 1501 tggcatgttc cgagacttcg gggaacccgg cccgagctcc gggaacggcg gcgggtacgg 1561 cggccccgcg cagcccccgg ccgcagcgca ggcagcccag cagaagttcc acctggtgcc 1621 aagcatcaac accatgagtg gcagtcagga gctgcagtgg atggtacagc ctcatttcct 1681 ggggcccagc agttacccca ggcctctgac ctaccctcag tacagccccc cacaaccccg 1741 gccaggagtc atccgggccc tggggccgcc tccaggggta cgtcgaaggc cttgtgaaca 1801 gatcagcccg gaggaagagg agcgccgccg agtaaggcgc gagcggaaca agctggctgc 1861 ggccaagtgc aggaaccgga ggaaggaact gaccgacttc ctgcaggcgg agactgacaa 1921 actggaagat gagaaatctg ggctgcagcg agagattgag gagctgcaga agcagaagga 1981 gcgcctagag ctggtgctgg aagcccaccg acccatctgc aaaatcccgg aaggagccaa 2041 ggagggggac acaggcagta ccagtggcac cagcagccca ccagccccct gccgccctgt 2101 accttgtatc tccctttccc cagggcctgt gcttgaacct gaggcactgc acacccccac 2161 actcatgacc acaccctccc taactccttt cacccccagc ctggtcttca cctaccccag 2221 cactcctgag ccttgtgcct cagctcatcg caagagtagc agcagcagcg gagacccatc 2281 ctctgacccc cttggctctc caaccctcct cgctttgtgc ccaactttct tgtacaaagt 2341 ggttcgataa cgaattccgc cccccccccc taacgttact ggccgaagcc gcttggaata 2401 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt 2461 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct 2521 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc 2581 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga 2641 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc 2701 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt 2761 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg 2821 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc 2881 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatga 2941 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca 3001 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc 3061 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg 3121 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg 3181 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc 3241 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg 3301 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg 3361 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga 3421 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg 3481 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgatgca 3541 tcgatgtcga cctgcagcca agcttatcga taaaataaaa gattttattt agtctccaga 3601 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat 3661 tttgcaaggc atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac 3721 agagagacag cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc 3781 tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac 3841 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta 3901 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag 3961 agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc 4021 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga 4081 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatgggt aacagtttct 4141 tgaagttgga gaacaacatt ctgagggtag gagtcgaata ttaagtaatc ctgactcaat 4201 tagccactgt tttgaatcca catactccaa tactcctgaa atagttcatt atggacagcg 4261 cagaaagagc tggggagaat tgtgaaattg ttatccgctc acaattccac acaacatacg 4321 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 4381 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 4441 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 4501 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 4561 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 4621 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 4681 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 4741 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 4801 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 4861 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 4921 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 4981 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 5041 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 5101 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 5161 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 5221 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 5281 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 5341 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 5401 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 5461 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 5521 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 5581 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 5641 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 5701 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 5761 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 5821 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 5881 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 5941 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 6001 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 6061 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 6121 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 6181 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 6241 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 6301 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 6361 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 6421 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 6481 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 6541 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 6601 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 6661 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 6721 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 6781 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 6841 agggttttcc cagtcacgac gttgtaaaac gacggcgcaa ggaatggtgc atgcaaggag 6901 atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg 6961 ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg 7021 ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggcga 7081 ttagtccaat ttgttaaaga caggatatca gtggtccagg ctctagtttt gactcaacaa 7141 tatcaccagc tgaagcctat agagtacgag ccatagataa aataaaagat tttatttagt 7201 ctccagaaaa agggggg //