LOCUS Exported 8852 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION promoterless expression plasmid driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2. ACCESSION . VERSION . KEYWORDS ptdTHC SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8852) AUTHORS Hartman BH, Bscke R, Ellwanger DC, Keymeulen S, Scheibinger M, Heller S TITLE Fbxo2(VHC) mouse and embryonic stem cell reporter lines delineate in vitro-generated inner ear sensory epithelia cells and enable otic lineage selection and Cre-recombination. JOURNAL Dev Biol. 2018 Sep 1. pii: S0012-1606(18)30499-8. doi: 10.1016/j.ydbio.2018.08.013. PUBMED 30179592 REFERENCE 2 (bases 1 to 8852) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8852 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 28..402 /codon_start=1 /gene="HIST1H2BJ" /product="human histone H2B" /label=H2B /translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKV LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP GELAKHAVSEGTKAVTKYTSA" CDS 412..1839 /codon_start=1 /product="tandem dimeric (pseudo-monomeric) derivative of DsRed (Shaner et al., 2004)" /label=tdTomato /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE RSEGRHHLFLYGMDELYK" CDS 1849..1905 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 1906..2928 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" CDS 2941..2997 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 2998..4026 /codon_start=1 /product="site-specific recombinase" /label=Cre /note="Cre is a site-specific recombinase from bacteriophage P1. Recombination occurs at loxP sequences." /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE DGD" CDS 4039..4974 /codon_start=1 /product="mutated ligand-binding domain of the human estrogen receptor (Feil et al., 1997)" /label=ERT2 /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET DQSHLATAGSTSSHSLQKYYITGEAEGFPAT" primer_bind complement(5068..5087) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 5133..5188 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(5187..5206) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(5549..5565) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5549..5565) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(5562..5584) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 5573..5589 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5597..5627) /label=lac promoter /note="promoter for the E. coli lac operon" rep_origin complement(5675..6130) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5812..5833) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 6024..6043 /label=F1ori-R /note="F1 origin, reverse primer" promoter 6157..6261 /gene="bla" /label=AmpR promoter promoter 6263..6620 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(6286..6306) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 6471..6606 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6533..6552 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 6655..7449 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(6709..6728) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 7319..7338 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(7637..7656) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 7681..7728 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 8057..8645 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8546..8565 /label=pBR322ori-F /note="pBR322 origin, forward primer" misc_feature 8771..8848 /label=MCS /note="multiple cloning site" ORIGIN 1 ggcaaagaat tcatgaggcc ggccaccatg ccagagccag cgaagtctgc tcccgccccg 61 aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aagacggcaa gaagcgcaag 121 cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct 181 gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc 241 gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc 301 tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc 361 gtgtccgagg gtactaaggc cgtcaccaag tacaccagcg ctaaatccac catggtgagc 421 aagggagagg aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg 481 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag 541 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc 601 ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac 661 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc 721 ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg 781 aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc 841 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac 901 caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg 961 gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc 1021 tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac 1081 ctgttcctgg ggcatggcac cggcagcacc ggcagcggca gctccggcac cgcctcctcc 1141 gaggacaaca acatggccgt catcaaagag ttcatgcgct tcaaggtgcg catggagggc 1201 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc 1261 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc 1321 ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc 1381 gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag 1441 gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac 1501 aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc 1561 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag 1621 atccaccagg ccctgaagct gaaggacggc ggccgctacc tggtggagtt caagaccatc 1681 tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac 1741 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc 1801 caccacctgt tcctgtacgg catggacgag ctgtacaagg gaagcggagc tactaacttc 1861 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctatgaa aaagcctgaa 1921 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg 1981 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga 2041 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg 2101 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag 2161 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa 2221 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc 2281 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact 2341 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg 2401 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc 2461 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg 2521 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc 2581 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag 2641 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat 2701 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat 2761 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg 2821 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc 2881 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaacg cggctccggc 2941 gcaacaaatt tctctctcct caaacaagca ggcgatgttg aagaaaatcc cgggcccatg 3001 tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat 3061 gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc 3121 tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg 3181 aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc 3241 ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg 3301 tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga 3361 aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat 3421 ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg 3481 gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg 3541 gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa 3601 acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc 3661 gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg 3721 gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg 3781 gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag 3841 agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc 3901 gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc 3961 atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat 4021 ggcgatctcg agccatctgc tggagacatg agagctgcca acctttggcc aagcccgctc 4081 atgatcaaac gctctaagaa gaacagcctg gccttgtccc tgacggccga ccagatggtc 4141 agtgccttgt tggatgctga gccccccata ctctattccg agtatgatcc taccagaccc 4201 ttcagtgaag cttcgatgat gggcttactg accaacctgg cagacaggga gctggttcac 4261 atgatcaact gggcgaagag ggtgccaggc tttgtggatt tgaccctcca tgatcaggtc 4321 caccttctag aatgtgcctg gctagagatc ctgatgattg gtctcgtctg gcgctccatg 4381 gagcacccag tgaagctact gtttgctcct aacttgctct tggacaggaa ccagggaaaa 4441 tgtgtagagg gcatggtgga gatcttcgac atgctgctgg ctacatcatc tcggttccgc 4501 atgatgaatc tgcagggaga ggagtttgtg tgcctcaaat ctattatttt gcttaattct 4561 ggagtgtaca catttctgtc cagcaccctg aagtctctgg aagagaagga ccatatccac 4621 cgagtcctgg acaagatcac agacactttg atccacctga tggccaaggc aggcctgacc 4681 ctgcagcagc agcaccagcg gctggcccag ctcctcctca tcctctccca catcaggcac 4741 atgagtaaca aaggcatgga gcatctgtac agcatgaagt gcaagaacgt ggtgcccctc 4801 tatgacctgc tgctggaggc ggcggacgcc caccgcctac atgcgcccac tagccgtgga 4861 ggggcatccg tggaggagac ggaccaaagc cacttggcca ctgcgggctc tacttcatcg 4921 cattccttgc aaaagtatta catcacgggg gaggcagagg gtttccctgc cacagcttga 4981 tagcggccgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa 5041 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat 5101 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat 5161 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt 5221 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc 5281 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca 5341 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt 5401 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt 5461 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga 5521 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 5581 cgctcacaat tccacacaac atacgagccg gatgcataaa gtgtaaagcc tggggtgcct 5641 aatgagtgaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta 5701 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc 5761 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg 5821 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat 5881 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc 5941 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag 6001 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg 6061 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta 6121 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 6181 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 6241 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt 6301 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 6361 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 6421 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 6481 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 6541 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 6601 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt 6661 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat 6721 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag 6781 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac 6841 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac 6901 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc 6961 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg 7021 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag 7081 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat 7141 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag 7201 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc 7261 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg 7321 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg 7381 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag 7441 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat 7501 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc 7561 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc 7621 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga 7681 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg 7741 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg 7801 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg 7861 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga 7921 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 7981 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 8041 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 8101 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 8161 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 8221 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 8281 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 8341 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 8401 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 8461 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 8521 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 8581 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 8641 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 8701 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt 8761 agttattact agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga 8821 cggtaccgcg ggcccgggat ccaccggtcg cc //