LOCUS       Exported                7736 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses human androgen receptor (R617M, K618M, K632M, K633M) in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-hAR-R617M,K618M,K632M,K633M
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Zhou ZX, Sar M, Simental JA, Lane MV, Wilson EM
  TITLE     A ligand-dependent bipartite nuclear targeting signal in the human 
            androgen receptor. Requirement for the DNA-binding domain and 
            modulation by NH2-terminal and carboxyl-terminal sequences.
  JOURNAL   J Biol Chem. 1994 May 6;269(18):13115-23.
  PUBMED    8175737
REFERENCE   2  (bases 1 to 7736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        178..557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        558..761
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     712..732
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    3937..4559
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4136..4155)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(4583..4603)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4588..4917
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4768..4903
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4830..4849
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4955..4974)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4956..4974)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4988..5004)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4988..5004)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5001..5023)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5012..5028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5036..5066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5081..5102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5219..5236)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5390..5978)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5470..5489)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6149..7009)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6772..6791
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7010..7114)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7141..7596
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7228..7247)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7438..7459
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7722..8
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7736..17
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgcc aagctgatct atacattgaa tcaatattgg caattagcca
       61 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
      121 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac
      181 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      421 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      481 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
      541 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
      601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      661 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
      781 attcagatct gtccaccgtg tgtcttcttc tgcacgagac tttgaggctg tcagagcgct
      841 ttttgcgtgg ttgctcccgc aagtttcctt ctctggagct tcccgcaggt gggcagctag
      901 ctgcagcgac taccgcatca tcacagcctg ttgaactctt ctgagcaaga gaaggggagg
      961 cggggtaagg gaagtaggtg gaagattcag ccaagctcaa ggatggaagt gcagttaggg
     1021 ctgggaaggg tctaccctcg gccgccgtcc aagacctacc gaggagcttt ccagaatctg
     1081 ttccagagcg tgcgcgaagt gatccagaac ccgggcccca ggcacccaga ggccgcgagc
     1141 gcagcacctc ccggcgccag tttgctgctg ctgcagcagc agcagcagca gcagcagcag
     1201 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcaaga gactagcccc
     1261 aggcagcagc agcagcagca gggtgaggat ggttctcccc aagcccatcg tagaggcccc
     1321 acaggctacc tggtcctgga tgaggaacag caaccttcac agccgcagtc ggccctggag
     1381 tgccaccccg agagaggttg cgtcccagag cctggagccg ccgtggccgc cagcaagggg
     1441 ctgccgcagc agctgccagc acctccggac gaggatgact cagctgcccc atccacgttg
     1501 tccctgctgg gccccacttt ccccggctta agcagctgct ccgctgacct taaagacatc
     1561 ctgagcgagg ccagcaccat gcaactcctt cagcaacagc agcaggaagc agtatccgaa
     1621 ggcagcagca gcgggagagc gagggaggcc tcgggggctc ccacttcctc caaggacaat
     1681 tacttagggg gcacttcgac catttctgac aacgccaagg agttgtgtaa ggcagtgtcg
     1741 gtgtccatgg gcctgggtgt ggaggcgttg gagcatctga gtccagggga acagcttcgg
     1801 ggggattgca tgtacgcccc acttttggga gttccacccg ctgtgcgtcc cactccttgt
     1861 gccccattgg ccgaatgcaa aggttctctg ctagacgaca gcgcaggcaa gagcactgaa
     1921 gatactgctg agtattcccc tttcaaggga ggttacacca aagggctaga aggcgagagc
     1981 ctaggctgct ctggcagcgc tgcagcaggg agctccggga cacttgaact gccgtctacc
     2041 ctgtctctct acaagtccgg agcactggac gaggcagctg cgtaccagag tcgcgactac
     2101 tacaactttc cactggctct ggccggaccg ccgccccctc cgccgcctcc ccatccccac
     2161 gctcgcatca agctggagaa cccgctggac tacggcagcg cctgggcggc tgcggcggcg
     2221 cagtgccgct atggggacct ggcgagcctg catggcgcgg gtgcagcggg acccggttct
     2281 gggtcaccct cagccgccgc ttcctcatcc tggcacactc tcttcacagc cgaagaaggc
     2341 cagttgtatg gaccgtgtgg tggtggtggg ggtggtggcg gcggcggcgg cggcggcggc
     2401 ggcggcggcg gcggcggcgg cggcggcgag gcgggagctg tagcccccta cggctacact
     2461 cggccccctc aggggctggc gggccaggaa agcgacttca ccgcacctga tgtgtggtac
     2521 cctggcggca tggtgagcag agtgccctat cccagtccca cttgtgtcaa aagcgaaatg
     2581 ggcccctgga tggatagcta ctccggacct tacggggaca tgcgtttgga gactgccagg
     2641 gaccatgttt tgcccattga ctattacttt ccaccccaga agacctgcct gatctgtgga
     2701 gatgaagctt ctgggtgtca ctatggagct ctcacatgtg gaagctgcaa ggtcttcttc
     2761 aaaagagccg ctgaagggaa acagaagtac ctgtgcgcca gcagaaatga ttgcactatt
     2821 gataaattcc gaaggaaaaa ttgtccatct tgtcgtctta tgatgtgtta tgaagcaggg
     2881 atgactctgg gagcccggaa gcttatgatg cttggtaatc tgaaactaca ggaggaagga
     2941 gaggcttcca gcaccaccag ccccactgag gagacaaccc agaagctgac agtgtcacac
     3001 attgaaggct atgaatgtca gcccatcttt ctgaatgtcc tggaagccat tgagccaggt
     3061 gtagtgtgtg ctggacacga caacaaccag cccgactcct ttgcagcctt gctctctagc
     3121 ctcaatgaac tgggagagag acagcttgta cacgtggtca agtgggccaa ggccttgcct
     3181 ggcttccgca acttacacgt ggacgaccag atggctgtca ttcagtactc ctggatgggg
     3241 ctcatggtgt ttgccatggg ctggcgatcc ttcaccaatg tcaactccag gatgctctac
     3301 ttcgcccctg atctggtttt caatgagtac cgcatgcaca agtcccggat gtacagccag
     3361 tgtgtccgaa tgaggcacct ctctcaagag tttggatggc tccaaatcac cccccaggaa
     3421 ttcctgtgca tgaaagcact gctactcttc agcattattc cagtggatgg gctgaaaaat
     3481 caaaaattct ttgatgaact tcgaatgaac tacatcaagg aactcgatcg tatcattgca
     3541 tgcaaaagaa aaaatcccac atcctgctca agacgcttct accagctcac caagctcctg
     3601 gactccgtgc agcctattgc gagagagctg catcagttca cttttgacct gctaatcaag
     3661 tcacacatgg tgagcgtgga ctttccggaa atgatggcag agatcatctc tgtgcaagtg
     3721 cccaagatcc tttctgggaa agtcaagccc atctatttcc acacccagtg aagcattgga
     3781 aaccctattt ccccacccca gctcatgccc cctttcagat gtcttctgcc tgttataact
     3841 ctgcactact cctctgcagt gccttgggga atttcctcta ttgatgtaca gtctgtcatg
     3901 agcttgcatg cctgcaggtc gactctagag gatcccgggt ggcatccctg tgacccctcc
     3961 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
     4021 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg
     4081 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat
     4141 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg
     4201 gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc
     4261 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg
     4321 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta
     4381 caggcgtgaa ccactgctcc cttccctgtc cttctgattt taaaataact ataccagcag
     4441 gaggacgtcc agacacagca taggctacct ggccatgccc aaccggtggg acatttgagt
     4501 tgcttgcttg gcactgtcct ctcatgcgtt gggtccactc agtagatgcc tgttgaattg
     4561 ggtacgcggc cagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     4621 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     4681 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4741 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4801 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     4861 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     4921 ctcctcgagg aactgaaaaa ccagaaagtt aattccctat agtgagtcgt attaaattcg
     4981 taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5041 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5101 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     5161 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5221 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5281 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5341 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5401 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5461 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5521 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5581 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5641 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5701 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5761 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5821 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5881 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5941 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6001 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6061 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6121 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6181 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6241 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6301 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6361 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6421 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     6481 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     6541 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     6601 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     6661 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     6721 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     6781 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     6841 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     6901 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     6961 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7021 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7081 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7141 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7201 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7261 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7321 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7381 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7441 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7501 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7561 acgcgaattt taacaaaata ttaacgctta caatttccat tcgccattca ggctgcgcaa
     7621 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     7681 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgtt
//
