LOCUS Exported 6012 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Red fluorescent voltage indicator for neuronal expression. ACCESSION . VERSION . KEYWORDS pAAV-Syn-VARNAM SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6012) AUTHORS Kannan, Madhuvanthi, Vasan, Ganesh, Huang, Cheng, Haziza, Simon, Li, Jin Zhong, Inan, Hakan, Schnitzer, Mark J, Pieribone, Vincent A TITLE Fast, in vivo voltage imaging using a red fluorescent indicator JOURNAL Nature Methods, 2018 REFERENCE 2 (bases 1 to 6012) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6012 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 44..173 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" promoter 218..665 /label=hSyn promoter /note="human synapsin I promoter; confers neuron-specific expression (Kugler et al., 2003)" regulatory 685..694 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" misc_feature 2182..2770 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2235..2255) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2653..2664) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 2802..3278 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3001..3020) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 3318..3458 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3318..3447 /label=AAV2 ITR rep_origin 3533..3988 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3620..3639) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3830..3851 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4005..4024) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4124..4146 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4184..4202) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4270..4374 /gene="bla" /label=AmpR promoter CDS 4375..5235 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4593..4612) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5406..5994 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5895..5914 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtcctgcag gcagctgcgc 61 gctcgctcgc tcactgaggc cgcccgggcg tcgggcgacc tttggtcgcc cggcctcagt 121 gagcgagcga gcgcgcagag agggagtggc caactccatc actaggggtt cctgcggccg 181 cacgcgtgtg tctagactgc agagggccct gcgtatgagt gcaagtgggt tttaggacca 241 ggatgaggcg gggtgggggt gcctacctga cgaccgaccc cgacccactg gacaagcacc 301 caacccccat tccccaaatt gcgcatcccc tatcagagag ggggagggga aacaggatgc 361 ggcgaggcgc gtgcgcactg ccagcttcag caccgcggac agtgccttcg cccccgcctg 421 gcggcgcgcg ccaccgccgc ctcagcactg aaggcgcgct gacgtcactc gccggtcccc 481 cgcaaactcc ccttcccggc caccttggtc gcgtccgcgc cgccgccggc ccagccggac 541 cgcaccacgc gaggcgcgag ataggggggc acgggcgcga ccatctgcgc tgcggcgccg 601 gcgactcagc gctgcctcag tctgcggtgg gcagcggagg agtcgtgtcg tgcctgagag 661 cgcagtcgag aaggtaccgg atccgccacc atggctgacg tggaaaccga gaccggcatg 721 attgcacagt ggattgtctt tgctattatg gctgctgctg ctattgcttt tggagtggct 781 gtgcactttc ggccttcaga gctgaagagc gcatactata tcaacattgc catctgcact 841 atcgccgcta ccgcttacta tgcaatggcc gtgaactacc aggacctgac aatgaatggt 901 gaaaggcagg tggtctacgc aagatatatt agctgggtgc tgaccacacc actgctcctg 961 ctcgatctca tcgtcatgac caagatgggc ggagtgatga tttcttgggt catcggcgca 1021 gacattttca tgatcgtgtt tggtattctg ggcgccttcg aggatgaaca caagttcaaa 1081 tgggtgtact ttatcgctgg atgtgtgatg caggcagtcc tgacatacgg gatgtataac 1141 gccacttgga aagacgatct gaagaaaagc cccgagtacc atagctccta tgtcagtctg 1201 ctcgtcttcc tgtcaatcct ctgggtgttt tatcctgtcg tgtgggcttt cgggtctggt 1261 agtggcgtgc tgtccgtcga caatgaggcc attctcatgg gaatcctgga tgtgctcgct 1321 aagccactgt ttggaatggg gtgcctcatt gcccatgaga ctatcttcaa gtggaggtcc 1381 ggactgatta aggaaaatat gcggatgaag gtcgtgatgg aagggtctgt caatgggcac 1441 cagttcaagt gcaccggaga gggagagggc aggccatacg agggcgtgca gacaatgagg 1501 atcaaagtga tcgagggagg accactgcct ttcgcctttg acatcctggc caccagcttc 1561 atgtacggca gcaggacctt catcaagtat ccagccgaca tccccgattt ctttaagcag 1621 agcttccccg agggctttac ctgggagagg gtgacaagat acgaggatgg cggcgtggtg 1681 accgtgacac aggacacctc tctggaggat ggcgagctgg tgtataacgt gaaggtgagg 1741 ggcgtgaact tccctagcaa tggcccagtg atgcagaaga agaccaaggg ctgggagccc 1801 aatacagaga tgatgtaccc tgcagacgga ggcctgaggg gatataccga catcgccctg 1861 aaggtggatg gaggaggaca cctgcactgt aactttgtga ccacataccg ctccaagaag 1921 acagtgggca atatcaagat gccaggagtg cacgccgtgg accacaggct ggagcgcatc 1981 gaggagtctg ataacgagac atatgtggtg cagagagagg tggccgtggc caagtactct 2041 aatctgggcg gcgggatgga cgagctgtat aagaagagca ggatcaccag cgagggcgag 2101 tacatccccc tggaccagat cgacatcaac gtgttctgct acgagaacga ggtgtaagaa 2161 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact 2221 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 2281 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 2341 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 2401 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 2461 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 2521 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca 2581 tcgtcctttc cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc 2641 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 2701 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc 2761 gcctccccgc atcgataccg agcgctgctc gagagatcta cgggtggcat ccctgtgacc 2821 cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc 2881 taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt 2941 ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg 3001 tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct 3061 cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca 3121 tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca 3181 ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg 3241 gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccacgt 3301 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc 3361 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc 3421 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct 3481 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc 3541 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 3601 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 3661 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 3721 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc 3781 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 3841 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt 3901 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 3961 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat 4021 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct 4081 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt 4141 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta 4201 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 4261 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 4321 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 4381 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 4441 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 4501 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 4561 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 4621 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 4681 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 4741 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 4801 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 4861 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 4921 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 4981 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 5041 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 5101 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 5161 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 5221 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 5281 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 5341 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 5401 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 5461 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 5521 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 5581 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 5641 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 5701 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 5761 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 5821 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 5881 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 5941 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 6001 agcaacgcgg cc //