LOCUS Exported 972 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pSKDuet01 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 972) AUTHORS Iwai H, Zuger S, Jin J, Tam PH TITLE Highly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. JOURNAL FEBS Lett. 2006 Mar 20. 580(7):1853-8. PUBMED 16516207 REFERENCE 2 (bases 1 to 972) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..972 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 174..198 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(199..217) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS complement(773..790) /codon_start=1 /product="thrombin recognition and cleavage site" /label=thrombin site /translation="LVPRGS" CDS complement(800..817) /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" protein_bind 873..897 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(898..916) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 ggtatagctg cgctgtagac gagtccatgt gctggcggtt caaatttcgc agcagcggtt 61 tctttaccag actcgagggt accgacgtca gcgatcgcgt ggccggccga tatccaattg 121 agatctgcca tatgtatatc tccttcttat acttaactaa tatactaaga tggggaattg 181 ttatccgctc acaattcccc tatagtgagt cgtattaatt tcgattatgc ggccgtgtac 241 aatacgatta ctttctgttc gacttaagca ttatgcggcc gcaagcttaa ttcggcaaat 301 tatcaacccg catcaaatcc aattcacgtt caaatatttc atcaattggc aacatttgac 361 catcaacagt cataaactta tggtcttttg ttgcccgaat caatgaacca tcttccaaac 421 aatactcaaa cacctcttgt tctccgcgat cgtgccattg tgctacaggt tgtgtataaa 481 tatttccatt attatcaacg ctataaacag tacattcgat gcgcttttct acaattttac 541 caatcggtaa taatccatat tctactgtca atatttccgt ttcatagctt aaacaggatc 601 cttccgttac ggtgtaggtt ttggtagcgt cgtcgtaggt ccattcaccg tcaacaccgt 661 tgtcgttagc gtactgtttg aaaacttttt ccgcggtagc agcgtcaaca gcttcggtgg 721 tggtttcacc tttcagggtt ttaccgttca ggataagttt gtactgcata tggctgccgc 781 gcggcaccag gccgctgctg tgatgatgat gatgatggct gctgcccatg gtatatctcc 841 ttattaaagt taaacaaaat tatttctaca ggggaattgt tatccgctca caattcccct 901 atagtgagtc gtattaattt cctaatgcag gagtcgcata agggagagcg tcgagatccc 961 ggacacatcg aa //