LOCUS       Exported                6245 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Cre-dependant expression of Voltron in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-hsyn-flex-Voltron
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Abdelfattah AS, Kawashima T, Singh A, Novak O, Liu H, Shuai Y, Huang
            YC, Campagnola L, Seeman SC, Yu J, Zheng J, Grimm JB, Patel R, 
            Friedrich J, Mensh BD, Paninski L, Macklin JJ, Murphy GJ, Podgorski 
            K, Lin BJ, Chen TW, Turner GC, Liu Z, Koyama M, Svoboda K, Ahrens 
            MB, Lavis LD, Schreiter ER
  TITLE     Bright and photostable chemigenetic indicators for extended in vivo 
            voltage imaging.
  JOURNAL   Science. 2019 Aug 1. pii: science.aav6416. doi: 
            10.1126/science.aav6416.
  PUBMED    31371562
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   56..185
                     /label=AAV2 ITR
     promoter        267..714
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    complement(744..777)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     protein_bind    complement(840..873)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      2539..2548
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     protein_bind    2559..2592
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     protein_bind    2663..2696
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    2738..3326
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2791..2811)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3209..3220)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3329..3345)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3330..3346)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3375..3496
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3386..3405)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3440..3459
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3658..3787
                     /label=AAV2 ITR
     rep_origin      3859..4314
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3946..3965)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4156..4177
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4340..4444
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4445..5305
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4663..4682)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5476..6064
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5965..5984
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6218..6235
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg
       61 cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg
      121 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg
      181 ttccttgtag ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa
      241 gatctctgca gagggccctg cgtatgagtg caagtgggtt ttaggaccag gatgaggcgg
      301 ggtgggggtg cctacctgac gaccgacccc gacccactgg acaagcaccc aacccccatt
      361 ccccaaattg cgcatcccct atcagagagg gggaggggaa acaggatgcg gcgaggcgcg
      421 tgcgcactgc cagcttcagc accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc
      481 caccgccgcc tcagcactga aggcgcgctg acgtcactcg ccggtccccc gcaaactccc
      541 cttcccggcc accttggtcg cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg
      601 aggcgcgaga taggggggca cgggcgcgac catctgcgct gcggcgccgg cgactcagcg
      661 ctgcctcagt ctgcggtggg cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaat
      721 tcaagctgct agcaaggatc cccataactt cgtataaagt atcctatacg aagttatatc
      781 aaaataggaa gaccaatgct tcaccatcga cccgaattgc caagcatcac catcgaccca
      841 taacttcgta taatgtatgc tatacgaagt tatactagag cttttacacc tcgttctcgt
      901 agcagaacac gttgatgtcg atctggtcca gggggatgta ctcgccctcg ctggtgatcc
      961 tgctcttagt ggttggctcg ccggaaatct cgagcgtcga cagccagcgc gcgatctcgc
     1021 tgccgatcag gtccgggttg tcttcttgca gcagattcag acccgggccg atgtccacag
     1081 ccttgcagtt aggcaggctt ttggccaggc gagcggcttc ggccggtggg atcagaacgc
     1141 ctggggtgcc ccagaacagc agcttcggga caggggactg gtgcagccag tccatgtatt
     1201 cttcgaccag cgcgacgatg ttcgctggct caccggcgat tggcagctcg tttgggaagc
     1261 gccacagtgg ctcgcggtca acaggattca ggaacggctc gcggtaatgg tccatctcga
     1321 cttcagtcag cgggcggacg acacccatcg gcagcgtacc ctcgataaaa acgttctgat
     1381 cgatgatcag cttgcggccg acgtcggtgg tgcggaaggc ctggaaggtc tcgcgggcaa
     1441 attctggcca ttcgtcccag gtcgggatag ggcggatgaa ctccataaat gcaatacctt
     1501 tgacgcgctc tggattgcgc ttggcccagt ggaaacccag agcggagccc cagtcgtgaa
     1561 tgaccaggac gacctcttcc agacccaggg cttcgatgaa ggcatccatg aagcggacgt
     1621 ggtcgtcgaa gaaataaccc aggtctggtt tgtcggattt gcccataccg atcaggtctg
     1681 gagcaatgca gcgatgggtc ggtgcaacat gcgggatgat gttgcgccac acgtaggagg
     1741 aggtcgggtt accgtgcagg aacagcacag gggtgccatc gcgcggacca acatcgacgt
     1801 agtgcatgcg ctcgcccagg acttccacat aatgggggtc gaatggaaag ccagtaccga
     1861 tcttgaagat agtctcatgg gcaatgaggc accccattcc aaacagtggc ttagcgagca
     1921 catccaggat tcccatgaga atggcctcat tgtcgacgga cagcacgcca ctaccagacc
     1981 cgaaagccca cacgacagga taaaacaccc agaggattga caggaagacg agcagactga
     2041 cataggagct atggtactcg gggcttttct tcagatcgtc tttccaagtg gcgttataca
     2101 tcccgtatgt caggactgcc tgcatcacac atccagcgat aaagtacacc catttgaact
     2161 tgtgttcatc ctcgaaggcg cccagaatac caaacacgat catgaaaatg tctgcgccga
     2221 tgacccaaga aatcatcact ccgcccatct tggtcatgac gatgagatcg agcaggagca
     2281 gtggtgtggt cagcacccag ttaatatatc ttgcgtagac cacctgcctt tcaccattca
     2341 ttgtcaggtc ctggtagttc acggccattg catagtaagc ggtagcggcg atagtgcaga
     2401 tggcaatgtt gatatagtat gcgctcttca gctctgaagg ccgaaagtgc acagccactc
     2461 caaaagcaat agcagcagca gccataatag caaagacaat ccactgtgca atcatgccgg
     2521 tctcggtttc cacgtcagcc atggtggcgg atcctagtat aacttcgtat aggatacttt
     2581 atacgaagtt atcattggga ttcttcctat tttgatccaa gcatcaccat cgaccctcta
     2641 gtccagatct caccatcgac ccataacttc gtatagcata cattatacga agttatgtcc
     2701 ctcgaagagg ttcgaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     2761 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     2821 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     2881 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     2941 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     3001 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     3061 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     3121 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     3181 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     3241 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     3301 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgacccg ggcggccgct
     3361 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     3421 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     3481 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     3541 gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga
     3601 tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg
     3661 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     3721 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     3781 cgcgcagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     3841 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3901 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     3961 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4021 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4081 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4141 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4201 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4261 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     4321 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4381 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4441 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4501 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4561 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4621 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4681 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4741 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4801 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4861 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4921 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4981 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5041 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5101 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5161 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5221 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5281 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5341 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5401 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5461 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5521 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5581 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     5641 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5701 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5761 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5821 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5881 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5941 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6001 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6061 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6121 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6181 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6241 ggaag
//
