LOCUS Exported 7208 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Plasmid name in publication: pAAV-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labeling. ACCESSION . VERSION . KEYWORDS pAAV-SYP-HRP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7208) AUTHORS Zhang Q, Lee WA, Paul DL, Ginty DD TITLE Multiplexed peroxidase-based electron microscopy labeling enables simultaneous visualization of multiple cell types. JOURNAL Nat Neurosci. 2019 Mar 18. pii: 10.1038/s41593-019-0358-7. doi: 10.1038/s41593-019-0358-7. PUBMED 30886406 REFERENCE 2 (bases 1 to 7208) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7208 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" promoter 231..1409 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 462..1400 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1357..1377 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" regulatory 1429..1438 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" misc_feature 3335..3923 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3388..3408) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3806..3817) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3955..4431 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(4154..4173) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 4471..4611 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 4471..4600 /label=AAV2 ITR rep_origin 4686..5141 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4773..4792) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4983..5004 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5158..5177) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 5277..5299 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(5337..5355) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 5423..5527 /gene="bla" /label=AmpR promoter CDS 5528..6388 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5746..5765) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6559..7147 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7048..7067 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtaagctt tgcaaagatg gataaagttt taaacagaga 181 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 241 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 301 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 361 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 421 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 481 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 541 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 601 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 661 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 721 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 781 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 841 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 901 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 961 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 1021 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 1081 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 1141 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 1201 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 1261 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 1321 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 1381 gtttttttct tccatttcag gtgtcgtgag gtaccggatc ctctagaggc caccatggac 1441 gtggtgaatc agctggtggc tgggggtcag ttccgggtgg tcaaggagcc ccttggcttc 1501 gtgaaggtgc tgcagtgggt ctttgccatc ttcgcctttg ctacgtgcgg cagctacacc 1561 ggagagcttc ggctgagcgt ggagtgtgcc aacaagacgg agagtgccct caacatcgaa 1621 gtcgaatttg agtacccatt caggctgcac caagtgtact ttgatgcacc ctcctgcgtt 1681 aaagggggca ctaccaagat cttcctagtt ggtgactact cctcctcggc tgaattcttt 1741 gtcaccgtgg ctgtgtttgc cttcctctac tccatggggg ccctggccac ctacatcttc 1801 ctgcagaaca agtaccgaga gaacaacaaa gggccaatga tggacttcct ggccacagca 1861 gtgttcgctt tcatgtggct agttagctca tccgcctggg ccaaaggcct gtccgatgtg 1921 aagatggcca ctgacccaga gaacattatc aaggagatgc ctatgtgccg ccagacagat 1981 atggatctag ccacccagtt aacccctaca ttctacgaca atagctgtcc caacgtgtcc 2041 aacatcgttc gcgacacaat cgtcaacgag ctcagatccg atcccaggat cgctgcttca 2101 atattacgtc tgcacttcca tgactgcttc gtgaatggtt gcgacgctag catattactg 2161 gacaacacca ccagtttccg cactgaaaag gatgcattcg ggaacgctaa cagcgccagg 2221 ggctttccag tgatcgatcg catgaaggct gccgttgagt cagcatgccc acgaacagtc 2281 agttgtgcag acctgctgac tatagctgcg caacagagcg tgactcttgc aggcggaccg 2341 tcctggagag tgccgctcgg tcgacgtgac tccctacagg cattcctaga tctggccaac 2401 gccaacttgc ctgctccatt cttcaccctg ccccagctga aggatagctt tagaaacgtg 2461 ggtctgaatc gctcgagtga ccttgtggct ctgtccggag gacacacatt tggaaagaac 2521 cagtgtaggt tcatcatgga taggctctac aatttcagca acactgggtt acctgacccc 2581 acgctgaaca ctacgtatct ccagacactg agaggcttgt gcccactgaa tggcaacctc 2641 agtgcactag tggactttga tctgcggacc ccaaccatct tcgataacaa gtactatgtg 2701 aatctagagg agcagaaagg cctgatacag agtgatcaag aactgtttag cagtccaaac 2761 gccactgaca ccatcccact ggtgagaagt tttgctaact ctactcaaac cttctttaac 2821 gccttcgtgg aagccatgga ccgtatgggt aacattaccc ctctgacggg tacccaaggc 2881 cagattcgtc tgaactgcag agtggtcaac agcaactcta ccggtggagc tcgaatcaca 2941 tgcaaggaac tgagggaccc tgtgacttca ggactcaaca cctcggtggt gtttggcttc 3001 ctgaacctgg tgctctgggt tggcaaccta tggttcgtgt tcaaggagac aggctgggcc 3061 gccccattca tgcgcgcacc tccaggcgcc ccagaaaagc aaccagctcc tggcgatgcc 3121 tacggcgatg cgggctatgg gcagggcccc ggaggctatg ggccccagga ctcctacggg 3181 cctcagggtg gttatcaacc cgattacggg cagccagcca gcggcggtgg cggtggctac 3241 gggcctcagg gcgactatgg gcagcaaggc tacggccaac agggtgcgcc cacctccttc 3301 tccaatcaga tgtaacgata tcaagcttat cgataatcaa cctctggatt acaaaatttg 3361 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 3421 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 3481 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 3541 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 3601 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 3661 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 3721 gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg 3781 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 3841 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 3901 ctccctttgg gccgcctccc cgcatcgata ccgagcgctg ctcgagagat ctacgggtgg 3961 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc 4021 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat 4081 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt 4141 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact 4201 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga 4261 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc 4321 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct 4381 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgattttg 4441 taggtaacca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc 4501 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg 4561 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga 4621 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc 4681 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 4741 gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct 4801 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 4861 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag 4921 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 4981 tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga 5041 tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa 5101 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac 5161 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc 5221 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 5281 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct 5341 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg 5401 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 5461 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 5521 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 5581 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 5641 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 5701 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 5761 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 5821 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 5881 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 5941 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 6001 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 6061 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 6121 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 6181 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 6241 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 6301 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 6361 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 6421 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 6481 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 6541 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 6601 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 6661 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 6721 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 6781 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 6841 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 6901 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 6961 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 7021 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 7081 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 7141 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 7201 tcacatgt //