LOCUS Exported 9908 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION drives expression of V5-NES-APEX2-GFP from human PGK promotor. ACCESSION . VERSION . KEYWORDS hPGK_V5-APEX2-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9908) AUTHORS Markmiller S, Soltanieh S, Server KL, Mak R, Jin W, Fang MY, Luo EC, Krach F, Yang D, Sen A, Fulzele A, Wozniak JM, Gonzalez DJ, Kankel MW, Gao FB, Bennett EJ, Lecuyer E, Yeo GW TITLE Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules. JOURNAL Cell. 2018 Jan 25;172(3):590-604.e13. doi: 10.1016/j.cell.2017.12.032. PUBMED 29373831 REFERENCE 2 (bases 1 to 9908) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9908 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(29..51) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 151..170 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 364..386 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 378..395 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 403..903 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 822..840 /label=hPGK-F /note="Human PGK promoter, forward primer" CDS 913..954 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" CDS 955..1701 /codon_start=1 /product="soybean ascorbate peroxidase, engineered to serve as a monomeric tag for electron microscopy and live-cell proteomics (Lam et al., 2015)" /label=APEX2 /note="more active than the original APEX due to the A134P mutation" /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK YAADEDAFFADYAEAHQKLSELGFADA" CDS 1708..1734 /codon_start=1 /product="nuclear export signal from the HIV Rev protein (Fischer et al., 1995)" /label=NES /translation="LPPLERLTL" CDS 1792..2454 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /label=CopGFP /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" misc_feature 2545..3133 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS complement(3016..3027) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 3207..3440 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 3512..3633 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" protein_bind 3720..3753 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." promoter 4107..4318 /label=EF-1-alpha core promoter /note="core promoter for human elongation factor EF-1-alpha" LTR 4331..4599 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" polyA_signal 5356..5477 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" protein_bind 5977..6010 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." promoter 6068..6567 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(6103..6126) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 6500..6519 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 6594..7166 /codon_start=1 /gene="Corynebacterium diphtheriae tox" /product="diphtheria toxin fragment A" /label=DTA /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI NFETRGKRGQDAMYEYMAQACAGNRVRR" intron 7243..7308 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" primer_bind complement(7376..7393) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7382..7606 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(7687..7703) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7687..7703) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7700..7722) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7711..7727 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7735..7765) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7780..7801 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7918..7935) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8089..8677) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8169..8188) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8848..9708) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9471..9490 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9709..9813) /gene="bla" /label=AmpR promoter primer_bind 9881..9899 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgttgg ggttgcgcct tttccaaggc 421 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc 481 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc 541 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc 601 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc 661 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat 721 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg 781 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag 841 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc 901 caggaattca tgggtaagcc tatccctaac cctctcctcg gtctcgattc tacgggaaag 961 tcttacccaa ctgtgagtgc tgattaccag gacgccgttg agaaggcgaa gaagaagctc 1021 agaggcttca tcgctgagaa gagatgcgct cctctaatgc tccgtttggc attccactct 1081 gctggaacct ttgacaaggg cacgaagacc ggtggaccct tcggaaccat caagcaccct 1141 gccgaactgg ctcacagcgc taacaacggt cttgacatcg ctgttaggct tttggagcca 1201 ctcaaggcgg agttccctat tttgagctac gccgatttct accagttggc tggcgttgtt 1261 gccgttgagg tcacgggtgg acctaaggtt ccattccacc ctggaagaga ggacaagcct 1321 gagccaccac cagagggtcg cttgcccgat cccactaagg gttctgacca tttgagagat 1381 gtgtttggca aagctatggg gcttactgac caagatatcg ttgctctatc tgggggtcac 1441 actattggag ctgcacacaa ggagcgttct ggatttgagg gtccctggac ctctaatcct 1501 cttattttcg acaactcata cttcacggag ttgttgagtg gtgagaagga aggtctcctt 1561 cagctacctt ctgacaaggc tcttttgtct gaccctgtat tccgccctct cgttgacaaa 1621 tatgcagcgg acgaagatgc cttctttgct gattacgctg aggctcacca aaagctttcc 1681 gagcttgggt ttgctgatgc cctgcagctg cctcccctgg agcgcctgac cctggacggt 1741 ggcggtggct cgggcggtgg tgggtcgatg gagagcgacg agagcggcct gcccgccatg 1801 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc 1861 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc 1921 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc 1981 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc 2041 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc 2101 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac 2161 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc 2221 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc 2281 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc 2341 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc 2401 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc 2461 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct 2521 cgctagagct gaatctaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg 2581 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 2641 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 2701 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 2761 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 2821 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 2881 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa 2941 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 3001 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 3061 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg 3121 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag 3181 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga 3241 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 3301 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 3361 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 3421 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 3481 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta 3541 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 3601 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc 3661 ctaactaaaa ttcgagctcg gtacctcgcg aatgcatcag atctttccac tcaaaacata 3721 taacttcgta tagcatacat tatacgaagt tatattcaaa aatctagaca gcggccgcca 3781 ctcaaaattt cttctataaa gtaacaaaac ttttatgagg gacagccccc ccccaaagcc 3841 cccagggatg taattacgtc cctcccccgc tagggggcag cagcgagccg cccggggctc 3901 cgctccggtc cggcgctccc cccgcatccc cgagccggca gcgtgcgggg acagcccggg 3961 cacggggaag gtggcacggg atcgctttcc tctgaacgct tctcgctgct ctttgagcct 4021 gcagacacct ggggggatac ggggaaaagg cctccaaggc cttaattcgg ccgcaaggat 4081 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa 4141 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg 4201 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat 4261 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct 4321 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca 4381 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc 4441 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg 4501 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta 4561 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac 4621 gatatcgcca ccatgacgga gtataagccg acagttaggc ttgctactag ggacgatgtg 4681 ccccgcgccg tccgcacact ggcagcagct tttgctgact accctgcaac acggcacacg 4741 gtggacccgg acaggcatat cgagagggta acagagctgc aggagctgtt cttgacgcgg 4801 gtaggtctgg atatcggtaa ggtttgggtg gcagacgacg gagcagctgt cgcggtgtgg 4861 accaccccgg agagcgttga ggcgggagcg gttttcgccg aaatcgggcc taggatggct 4921 gagttgtctg ggtccagact ggccgctcaa cagcaaatgg agggcttgct ggcaccgcat 4981 cgcccgaaag agccagcttg gttcctggca accgtgggcg tttcacccga tcaccaaggc 5041 aagggtctgg gaagtgcagt ggtgctgccc ggagtggaag ccgcagaacg cgcgggggtg 5101 cccgcatttc tggagacaag tgcccctagg aacctgccct tctatgagag acttggtttc 5161 accgttacag ctgacgtgga ggtgcccgag ggtccccgca cgtggtgcat gactcggaaa 5221 ccgggcgctt gaaatcaacc tctggacgaa attccgcggt tattacaaaa tttgtgaaag 5281 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat 5341 gcctttgtat cagttaactt gtttattgca gcttataatg gttacaaata aagcaatagc 5401 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 5461 ctcatcaatg tatcttatca tgtctggaat tgactcaaat gatgtcaatt agtctatcag 5521 aagctatctg gtctcccttc cgggggacaa gacatccctg tttaatattt aaacagcagt 5581 gttcccaaac tgggttctta tatcccttgc tctggtcaac caggttgcag ggtttcctgt 5641 cctcacagga acgaagtccc taaagaaaca gtggcagcca ggtttagccc cggaattgac 5701 tggattcctt ttttagggcc cattggtatg gctttttccc cgtatccccc caggtgtctg 5761 caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt 5821 gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc 5881 ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg 5941 gggctttggg ggggggctgt ccctgatatc aaacatataa cttcgtatag catacattat 6001 acgaagttat tttatcacgt aagtagaaca tgaaataacc tagatcggat ccccgtcgac 6061 tgcatgcggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc 6121 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc 6181 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc 6241 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa 6301 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta 6361 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg 6421 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga 6481 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc 6541 tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat cctgatgatg 6601 ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac gggactaaac 6661 ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt acacaaggaa 6721 attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac gctgcgggat 6781 actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt 6841 atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact attaagaaag 6901 agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa gagtttatca 6961 aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt 7021 ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta gaacttgaga 7081 ttaattttga aacccgtgga aaacgtggcc aagatgcgat gtatgagtat atggctcaag 7141 cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg 7201 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta 7261 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat 7321 ggaactgatg aatgggagca gtggtggaat gcagatccta gagctcgctg atcagcctcg 7381 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 7441 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 7501 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 7561 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 7621 agaaccagct ggggctcgag atccactagt tctagcctcg aggcatgcaa gcttggcgta 7681 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 7741 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 7801 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 7861 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 7921 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 7981 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 8041 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 8101 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 8161 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 8221 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 8281 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 8341 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 8401 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 8461 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 8521 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 8581 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 8641 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 8701 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 8761 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 8821 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 8881 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 8941 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 9001 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 9061 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 9121 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 9181 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 9241 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 9301 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 9361 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 9421 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 9481 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 9541 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 9601 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 9661 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 9721 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 9781 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 9841 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 9901 ctttcgtc //