LOCUS       Exported                1035 bp ds-DNA     linear   SYN 12-SEP-2021
DEFINITION  Level 0 Part. CDS.
ACCESSION   .
VERSION     .
KEYWORDS    pC0.286
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Vasudevan R, Gale GAR, Schiavon AA, Puzorjov A, Malin J, Gillespie 
            MD, Vavitsas K, Zulkower V, Wang B, Howe CJ, Lea-Smith DJ, McCormick
            AJ
  TITLE     CyanoGate: A Modular Cloning Suite for Engineering Cyanobacteria 
            Based on the Plant MoClo Syntax.
  JOURNAL   Plant Physiol. 2019 May;180(1):39-55. doi: 10.1104/pp.18.01401. Epub
            2019 Feb 28.
  PUBMED    30819783
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..103
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             104..763
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(170..189)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
       61 attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
      121 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
      181 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
      241 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
      301 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
      361 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
      421 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
      481 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
      541 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
      601 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
      661 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
      721 tgaattacaa cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta
      781 ttggtgccct taaacgcctg gttgctacgc ctgaataagt gataataagc ggatgaatgg
      841 cagaaattcg aaagcaaatt cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc
      901 gcttatgtct attgctggtt taccggttta ttgactaccg gaagcagtgt gaccgtgtgc
      961 ttctcaaatg cctgaggcca gtttgctcag gctctccccg tggaggtaat aattgacgat
     1021 atgatcgacg gatct
//
