LOCUS Exported 9782 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Constitutive expression of Renilla luciferase. ACCESSION . VERSION . KEYWORDS pLX313-Renilla luciferase SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9782) AUTHORS Giacomelli AO, Yang X, Lintner RE, McFarland JM, Duby M, Kim J, Howard TP, Takeda DY, Ly SH, Kim E, Gannon HS, Hurhula B, Sharpe T, Goodale A, Fritchman B, Steelman S, Vazquez F, Tsherniak A, Aguirre AJ, Doench JG, Piccioni F, Roberts CWM, Meyerson M, Getz G, Johannessen CM, Root DE, Hahn WC TITLE Mutational processes shape the landscape of TP53 mutations in human cancer. JOURNAL Nat Genet. 2018 Oct;50(10):1381-1387. doi: 10.1038/s41588-018-0204-y. PUBMED 30224644 REFERENCE 2 (bases 1 to 9782) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9782 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..181 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 228..353 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 846..1079 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1264..1308 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 1539..1749 /label=SV40 promoter /note="SV40 early promoter" promoter 1561..1757 /label=SV40 promoter /note="SV40 early promoter" rep_origin 1608..1743 /label=SV40 ori /note="SV40 origin of replication" CDS 1779..2798 /codon_start=1 /product="hygromycin B phosphotransferase" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK " misc_feature 2869..2986 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 3124..4302 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 3355..4293 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 4250..4270 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 4322..4346 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 4346..5281 /codon_start=1 /product="luciferase from the anthozoan coelenterate Renilla reniformis (sea pansy)" /label=Rluc /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" protein_bind complement(5284..5308) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 5316..5357 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5408..5996 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5461..5481) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5879..5890) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6068..6301 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 6379..6500 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6416..6435) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6470..6489 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 6540..6675 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(6695..6714) /label=T7 /note="T7 promoter, forward primer" promoter complement(6696..6714) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(6724..6741) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(6724..6740) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6733..6755) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 6882..7337 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6969..6988) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7179..7200 /label=F1ori-F /note="F1 origin, forward primer" promoter 7363..7467 /gene="bla" /label=AmpR promoter CDS 7468..8328 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7686..7705) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8499..9087 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8988..9007 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9241..9258 /label=L4440 /note="L4440 vector, forward primer" protein_bind 9375..9396 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9411..9441 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9449..9465 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9454..9476 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9473..9489 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9473..9489 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9508..9528 /label=T3 /note="T3 promoter, forward primer" promoter 9510..9528 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 9556..9782 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" ORIGIN 1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca 241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc 301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta 361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa 421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc 481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc 541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg 601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc 661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga 721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg catctcaatt 1501 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 1561 tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 1621 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 1681 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 1741 gcctaggctt ttgcaaaaag ctttctagag gtaccaccat gaaaaagcct gaactcaccg 1801 ctacctctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc 1861 tctccgaggg cgaagaatct cgggctttca gcttcgatgt gggagggcgt ggatatgtcc 1921 tgcgggtgaa tagctgcgcc gatggtttct acaaagatcg ctatgtttat cggcactttg 1981 catccgccgc tctccctatt cccgaagtgc ttgacattgg ggagttcagc gagagcctga 2041 cctattgcat ctcccgccgt gcacagggtg tcaccttgca agacctgcct gaaaccgaac 2101 tgcccgctgt tctccagccc gtcgccgagg ccatggatgc catcgctgcc gccgatctta 2161 gccagaccag cgggttcggc ccattcggac ctcaaggaat cggtcaatac actacatggc 2221 gcgatttcat ctgcgctatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg 2281 acaccgtcag tgcctccgtc gcccaggctc tcgatgagct gatgctttgg gccgaggact 2341 gccccgaagt ccggcacctc gtgcacgccg atttcggctc caacaatgtc ctgaccgaca 2401 atggccgcat aacagccgtc attgactgga gcgaggccat gttcggggat tcccaatacg 2461 aggtcgccaa catcttcttc tggaggccct ggttggcttg tatggagcag cagacccgct 2521 acttcgagcg gaggcatccc gagcttgcag gatctcctcg gctccgggct tatatgctcc 2581 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt 2641 gggctcaggg tcgctgcgac gcaatcgtcc ggtccggagc cgggactgtc gggcgtacac 2701 aaatcgcccg cagaagcgct gccgtctgga ccgatggctg tgtggaagtg ctcgccgata 2761 gtggaaacag acgccccagc actcgtccta gggcaaagga tctgcagtaa tgaggcgcgc 2821 cgttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa 2881 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat 2941 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag 3001 ggacagcaga gatccacttt ggcgccggct cgaggcctgc aggtgcaaag atggataaag 3061 ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga 3121 attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg 3181 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa 3241 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt 3301 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt 3361 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga 3421 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg 3481 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc 3541 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct 3601 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc 3661 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg 3721 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg 3781 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg 3841 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca 3901 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg 3961 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt 4021 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc 4081 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg 4141 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg 4201 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag 4261 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggctagca tcgattgatc 4321 aacaagtttg tacaaaaaag ttggcatgac ttcgaaagtt tatgatccag aacaaaggaa 4381 acggatgata actggtccgc agtggtgggc cagatgtaaa caaatgaatg ttcttgattc 4441 atttattaat tattatgatt cagaaaaaca tgcagaaaat gctgttattt ttttacatgg 4501 taacgcggcc tcttcttatt tatggcgaca tgttgtgcca catattgagc cagtagcgcg 4561 gtgtattata ccagacctta ttggtatggg caaatcaggc aaatctggta atggttctta 4621 taggttactt gatcattaca aatatcttac tgcatggttt gaacttctta atttaccaaa 4681 gaagatcatt tttgtcggcc atgattgggg tgcttgtttg gcatttcatt atagctatga 4741 gcatcaagat aagatcaaag caatagttca cgctgaaagt gtagtagatg tgattgaatc 4801 atgggatgaa tggcctgata ttgaagaaga tattgcgttg atcaaatctg aagaaggaga 4861 aaaaatggtt ttggagaata acttcttcgt ggaaaccatg ttgccatcaa aaatcatgag 4921 aaagttagaa ccagaagaat ttgcagcata tcttgaacca ttcaaagaga aaggtgaagt 4981 tcgtcgtcca acattatcat ggcctcgtga aatcccgtta gtaaaaggtg gtaaacctga 5041 cgttgtacaa attgttagga attataatgc ttatctacgt gcaagtgatg atttaccaaa 5101 aatgtttatt gaatcggacc caggattctt ttccaatgct attgttgaag gtgccaagaa 5161 gtttcctaat actgaatttg tcaaagtaaa aggtcttcat ttttcgcaag aagatgcacc 5221 tgatgaaatg ggaaaatata tcaaatcgtt cgttgagcga gttctcaaaa atgaacaata 5281 aacccccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct aaccctctcc 5341 tcggtctcga ttctacgtag taatgaacta gtaccggtta agtcgacaat caacgcgtta 5401 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 5461 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 5521 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 5581 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 5641 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 5701 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 5761 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg 5821 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 5881 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 5941 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg 6001 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 6061 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact 6121 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 6181 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 6241 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 6301 agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 6361 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 6421 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 6481 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca 6541 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 6601 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 6661 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg 6721 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa 6781 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga 6841 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc 6901 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 6961 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 7021 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 7081 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 7141 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 7201 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 7261 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 7321 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 7381 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 7441 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 7501 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 7561 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 7621 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 7681 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 7741 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 7801 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 7861 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 7921 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 7981 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 8041 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 8101 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 8161 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 8221 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 8281 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 8341 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 8401 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 8461 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 8521 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 8581 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 8641 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 8701 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 8761 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 8821 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 8881 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 8941 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 9001 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 9061 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 9121 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 9181 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 9241 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 9301 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 9361 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 9421 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac 9481 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg 9541 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga 9601 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag 9661 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac 9721 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa 9781 ac //