LOCUS Exported 9852 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION expression plasmid with CAG promoter driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2. ACCESSION . VERSION . KEYWORDS pCAG-EHC SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9852) AUTHORS Hartman BH, Bscke R, Ellwanger DC, Keymeulen S, Scheibinger M, Heller S TITLE Fbxo2(VHC) mouse and embryonic stem cell reporter lines delineate in vitro-generated inner ear sensory epithelia cells and enable otic lineage selection and Cre-recombination. JOURNAL Dev Biol. 2018 Sep 1. pii: S0012-1606(18)30499-8. doi: 10.1016/j.ydbio.2018.08.013. PUBMED 30179592 REFERENCE 2 (bases 1 to 9852) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9852 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 28..402 /codon_start=1 /gene="HIST1H2BJ" /product="human histone H2B" /label=H2B /translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKV LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP GELAKHAVSEGTKAVTKYTSA" CDS 412..1128 /codon_start=1 /product="brightly fluorescent monomeric variant of GFP" /label=mEmerald /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(457..478) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(718..737) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1065..1086 /label=EGFP-C /note="EGFP, forward primer" CDS 1138..1194 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 1195..2217 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" CDS 2230..2286 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 2287..3315 /codon_start=1 /product="site-specific recombinase" /label=Cre /note="Cre is a site-specific recombinase from bacteriophage P1. Recombination occurs at loxP sequences." /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE DGD" CDS 3328..4263 /codon_start=1 /product="mutated ligand-binding domain of the human estrogen receptor (Feil et al., 1997)" /label=ERT2 /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET DQSHLATAGSTSSHSLQKYYITGEAEGFPAT" primer_bind complement(4357..4376) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 4422..4477 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(4476..4495) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(4838..4854) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4838..4854) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4851..4873) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4862..4878 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4886..4916) /label=lac promoter /note="promoter for the E. coli lac operon" rep_origin complement(4964..5419) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5101..5122) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 5313..5332 /label=F1ori-R /note="F1 origin, reverse primer" promoter 5446..5550 /gene="bla" /label=AmpR promoter promoter 5552..5909 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(5575..5595) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 5760..5895 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5822..5841 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 5944..6738 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(5998..6017) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 6608..6627 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(6926..6945) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 6970..7017 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 7346..7934 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7835..7854 /label=pBR322ori-F /note="pBR322 origin, forward primer" enhancer 8108..8487 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 8490..8765 /label=chicken beta-actin promoter intron 8766..9774 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 9698..9720 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 9782..9801 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" ORIGIN 1 ggcaaagaat tcatgaggcc ggccaccatg ccagagccag cgaagtctgc tcccgccccg 61 aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aagacggcaa gaagcgcaag 121 cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct 181 gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc 241 gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc 301 tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc 361 gtgtccgagg gtactaaggc cgtcaccaag tacaccagcg ctaaatccac catggtgagc 421 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta 481 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg 541 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 601 accttgacct acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac 661 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac 721 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 781 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 841 tacaactaca acagccacaa ggtctatatc accgccgaca agcagaagaa cggcatcaag 901 gtgaacttca agacccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac 961 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 1021 acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 1081 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg aagcggagct 1141 actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg acctatgaaa 1201 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc 1261 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga 1321 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat 1381 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa 1441 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac 1501 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc 1561 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt 1621 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg 1681 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg 1741 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac 1801 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc 1861 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg 1921 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc 1981 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat 2041 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg 2101 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta 2161 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaacgc 2221 ggctccggcg caacaaattt ctctctcctc aaacaagcag gcgatgttga agaaaatccc 2281 gggcccatgt ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca 2341 acgagtgatg aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct 2401 gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg 2461 aataaccgga aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt 2521 caggcgcgcg gtctggcagt aaaaactatc cagcaacatt tgggccagct aaacatgctt 2581 catcgtcggt ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg 2641 cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa 2701 cgcactgatt tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata 2761 cgtaatctgg catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc 2821 aggatcaggg ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc 2881 agaacgaaaa cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact 2941 aaactggtcg agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg 3001 ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact 3061 cgcgccctgg aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac 3121 tctggtcaga gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat 3181 atggcccgcg ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta 3241 aatattgtca tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg 3301 ctggaagatg gcgatctcga gccatctgct ggagacatga gagctgccaa cctttggcca 3361 agcccgctca tgatcaaacg ctctaagaag aacagcctgg ccttgtccct gacggccgac 3421 cagatggtca gtgccttgtt ggatgctgag ccccccatac tctattccga gtatgatcct 3481 accagaccct tcagtgaagc ttcgatgatg ggcttactga ccaacctggc agacagggag 3541 ctggttcaca tgatcaactg ggcgaagagg gtgccaggct ttgtggattt gaccctccat 3601 gatcaggtcc accttctaga atgtgcctgg ctagagatcc tgatgattgg tctcgtctgg 3661 cgctccatgg agcacccagt gaagctactg tttgctccta acttgctctt ggacaggaac 3721 cagggaaaat gtgtagaggg catggtggag atcttcgaca tgctgctggc tacatcatct 3781 cggttccgca tgatgaatct gcagggagag gagtttgtgt gcctcaaatc tattattttg 3841 cttaattctg gagtgtacac atttctgtcc agcaccctga agtctctgga agagaaggac 3901 catatccacc gagtcctgga caagatcaca gacactttga tccacctgat ggccaaggca 3961 ggcctgaccc tgcagcagca gcaccagcgg ctggcccagc tcctcctcat cctctcccac 4021 atcaggcaca tgagtaacaa aggcatggag catctgtaca gcatgaagtg caagaacgtg 4081 gtgcccctct atgacctgct gctggaggcg gcggacgccc accgcctaca tgcgcccact 4141 agccgtggag gggcatccgt ggaggagacg gaccaaagcc acttggccac tgcgggctct 4201 acttcatcgc attccttgca aaagtattac atcacggggg aggcagaggg tttccctgcc 4261 acagcttgat agcggccgca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg 4321 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta 4381 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt 4441 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc 4501 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat 4561 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc 4621 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt 4681 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc 4741 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag 4801 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 4861 attgttatcc gctcacaatt ccacacaaca tacgagccgg atgcataaag tgtaaagcct 4921 ggggtgccta atgagtgaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat 4981 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc 5041 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt 5101 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa 5161 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg 5221 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga 5281 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct 5341 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat 5401 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt 5461 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 5521 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg 5581 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 5641 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 5701 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 5761 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 5821 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 5881 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt 5941 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 6001 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 6061 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 6121 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 6181 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 6241 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 6301 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 6361 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 6421 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc 6481 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 6541 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 6601 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 6661 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 6721 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca 6781 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 6841 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 6901 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc 6961 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata 7021 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg 7081 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc 7141 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata 7201 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 7261 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 7321 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 7381 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 7441 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 7501 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 7561 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 7621 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 7681 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 7741 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 7801 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 7861 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 7921 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 7981 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 8041 ccatgcatta gttattacta gcgctaccgg actcagatct cgagctcaag cttcgaattc 8101 tgcagtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt 8161 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga 8221 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca 8281 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca 8341 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg 8401 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc 8461 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct 8521 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg 8581 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg 8641 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa 8701 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc 8761 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc 8821 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc 8881 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg 8941 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg 9001 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc 9061 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg 9121 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg 9181 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca 9241 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg 9301 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc 9361 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc 9421 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg 9481 cagggacttc ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc 9541 cctctagcgg gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg 9601 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag 9661 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc 9721 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg 9781 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcct cgaggatgat 9841 ccaccggtcg cc //