LOCUS Exported 6417 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Localization: Focal Adhesions, Excitation: 530, Emission: 541. ACCESSION . VERSION . KEYWORDS mPapaya-Zyxin-6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6417) AUTHORS Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, Davidson MW, Campbell RE TITLE An engineered monomeric Zoanthus sp. yellow fluorescent protein. JOURNAL Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3. PUBMED 24094838 REFERENCE 2 (bases 1 to 6417) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6417 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 574..583 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 2304..2313 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2310..3023 /codon_start=1 /product="monomeric yellow fluorescent protein derived from the Zoanthus fluorescent protein zFP538" /label=mPapaya1 /note="mammalian codon-optimized" /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSFLF EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ GILKGKVKMYLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW QLTEHAIAGMDELYK" polyA_signal 3145..3266 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3182..3201) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3236..3255 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(3273..3728) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3410..3431) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3622..3641 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3755..3859 /gene="bla" /label=AmpR promoter promoter 3861..4218 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3884..3904) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 4069..4204 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4131..4150 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4253..5047 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4307..4326) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4917..4936 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(5235..5254) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 5279..5326 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5655..6243 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6144..6163 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 541 ccggactcag atctcgagct caagcttcga attccatggc ggccccccgc ccgtctcccg 601 cgatccccgt ttcggtctcg gctccggctt tttacgcccc gcagaagaag ttcggccctg 661 tggtggcccc aaagcccaaa gtgaatccct tccggcccag ggacagcgag cctcccccgg 721 cacccggggc ccagcgcgca cagatgggcc gggtgggcga tattcccccg ccgcccccgg 781 aagactttcc cctgcctcca cctccccttg ctggggatgg cgacgatgca gagggtgctc 841 tgggaggtgc cttcccgccg ccccctcccc cgatcgagga atcatttccc cctgcgcctc 901 tggaggagga gatcttccct tccccgccgc ctcctccgga ggaggaggga gggcctgagg 961 cccccatacc gcccccacca cagcccaggg agaaggtgag cagtattgat ttggagatcg 1021 actctctgtc ctcactgctg gatgacatga ccaagaatga tcctttcaaa gcccgggtgt 1081 catctggata tgtgccccca ccagtggcca ctccattcag ttccaagtcc agtaccaagc 1141 ctgcagccgg gggcacagca cccctgcctc cttggaagtc cccttccagc tcccagcctc 1201 tgccccaggt tccggctccg gctcagagcc agacacagtt ccatgttcag ccccagcccc 1261 agcccaagcc tcaggtccaa ctccatgtcc agtcccagac ccagcctgtg tctttggcta 1321 acacccagcc ccgagggccc ccagcctcat ctccggctcc agcccctaag ttttctccag 1381 tgactcctaa gtttactcct gtggcttcca agttcagtcc tggagcccca ggtggatctg 1441 ggtcacaacc aaatcaaaaa ttggggcacc ccgaagctct ttctgctggc acaggctccc 1501 ctcaacctcc cagcttcacc tatgcccagc agagggagaa gccccgagtg caggagaagc 1561 agcaccccgt gcccccaccg gctcagaacc aaaaccaggt gcgctcccct ggggccccag 1621 ggcccctgac tctgaaggag gtggaggagc tggagcagct gacccagcag ctaatgcagg 1681 acatggagca tcctcagagg cagaatgtgg ctgtcaacga actctgcggc cgatgccatc 1741 aacccctggc ccgggcgcag ccagccgtcc gcgctctagg gcagctgttc cacatcgcct 1801 gcttcacctg ccaccagtgt gcgcagcagc tccagggcca gcagttctac agtctggagg 1861 gggcgccgta ctgcgagggc tgttacactg acaccctgga gaagtgtaac acctgcgggg 1921 agcccatcac tgaccgcatg ctgagggcca cgggcaaggc ctatcacccg cactgcttca 1981 cctgtgtggt ctgcgcccgc cccctggagg gcacctcctt catcgtggac caggccaacc 2041 ggccccactg tgtccccgac taccacaagc agtacgcccc gaggtgctcc gtctgctctg 2101 agcccatcat gcctgagcct ggccgagatg agactgtgcg agtggtcgcc ctggacaaga 2161 acttccacat gaagtgttac aagtgtgagg actgcgggaa gcccctgtcg attgaggcag 2221 atgacaatgg ctgcttcccc ctggacggtc acgtgctctg tcggaagtgc cacactgcta 2281 gagcccagac ggatccaccg gtcgccacca tggtgagcaa gggcgagggg caatcgaagc 2341 atgggctaaa ggaggagatg acggtgaagt accacatgga aggttgcgtt aatggtcaca 2401 aatttgtcat tactggagag ggcattggaa acccttttaa gggtaagcaa actgcaaatt 2461 tgtgtgtgat agaaggaggc ccgctgccgt tctcggagga cattttaagt ccgggtttta 2521 aatatggtga ccggattttc acagagtacc cacaggatat tgtagattac ttcaagaact 2581 catgtccagc gggctatacg tgggaaagga gcttcctctt tgaggacgga gcggtctgtc 2641 gatgcaacgt ggacataaca gtctctgaaa aggagaactg catctatcac aaaagtatct 2701 tcagaggggt gaattttccc gccgacggcc ccgtaatgaa gaaaatgacc actaattggg 2761 aagctagtac cgagaaaatt gtgcctgttc caaagcaagg gatattaaaa ggaaaggtca 2821 aaatgtacct attgctgaag gatggcggtc gttatcattg ccagtttgat acggtatata 2881 aagctaaatc agtgccctcc aagatgccag aatggcattt tatacagcat aagctcctta 2941 gggaggaccg ctctgatgct aaaaaccaga aatggcaatt aacagaacat gcaatcgcag 3001 gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca 3061 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 3121 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 3181 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 3241 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa 3301 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 3361 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 3421 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 3481 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 3541 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 3601 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 3661 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 3721 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 3781 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 3841 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta 3901 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 3961 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 4021 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 4081 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 4141 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 4201 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga 4261 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 4321 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 4381 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga 4441 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 4501 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 4561 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 4621 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 4681 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 4741 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga 4801 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 4861 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 4921 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 4981 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 5041 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 5101 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 5161 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct 5221 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg 5281 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt 5341 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc 5401 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca 5461 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt 5521 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 5581 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 5641 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 5701 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 5761 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 5821 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 5881 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 5941 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 6001 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 6061 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 6121 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 6181 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 6241 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 6301 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcattag 6361 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccg //