LOCUS Exported 5212 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Localization: Light Chain Myosin, Excitation: 530, Emission: 541. ACCESSION . VERSION . KEYWORDS mPapaya1-LC-Myosin-C-10 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5212) AUTHORS Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, Davidson MW, Campbell RE TITLE An engineered monomeric Zoanthus sp. yellow fluorescent protein. JOURNAL Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3. PUBMED 24094838 REFERENCE 2 (bases 1 to 5212) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5212 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 1099..1108 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1105..1818 /codon_start=1 /product="monomeric yellow fluorescent protein derived from the Zoanthus fluorescent protein zFP538" /label=mPapaya1 /note="mammalian codon-optimized" /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSYLF EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ GILKGKVKMCLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW QLTEHAIAGMDELYK" polyA_signal 1940..2061 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1977..1996) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2031..2050 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2068..2523) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2205..2226) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2417..2436 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2550..2654 /gene="bla" /label=AmpR promoter promoter 2656..3013 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2679..2699) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2864..2999 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2926..2945 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3048..3842 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3102..3121) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3712..3731 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4030..4049) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4074..4121 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4450..5038 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4939..4958 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 541 ccggactcag atctcgagct caagcttatg tcgagcaaga gagccaaggc caagaccacc 601 aagaagaggc cccagagggc tacgtccaat gtcttcgcca tgtttgacca gtcccagatc 661 caggagttta aggaggcctt caacatgatt gatcagaacc gcgatggctt cattgataag 721 gaggacctgc acgacatgtt ggcctctctg gggaagaacc ccacagacga gtacctggag 781 ggcatgatga acgaggcgcc agggcccatc aacttcacca tgttcctcac aatgtttggg 841 gagaagctga acggcacaga ccccgaggat gtgatccgca atgcctttgc ctgctttgat 901 gaggaggcct caggcttcat ccacgaggac cacctgaggg agctgctcac caccatgggc 961 gaccgattca cggatgagga ggtggacgag atgtaccgcg aggcacccat tgataagaag 1021 ggcaacttca actacgtgga gttcactcgc atcctcaaac acggcgccaa ggacaaggac 1081 gacctggatc caccggtcgc caccatggtg agcaagggcg aggggcaatc gaagcatgga 1141 ctaaaggagg agatgacggt gaagtaccac atggaaggtt gcgttaatgg tcacaaattt 1201 gtcattactg gagagggcat tggaaaccct tttaagggta agcaaaccgc aaatttgtgt 1261 gtgatagaag gaggcccgct gccgttctcg gaggacattt taagtccggg ttttaaatat 1321 ggtgaccgga ttttcacaga gtacccacag gatattgtag attacttcaa gaactcatgt 1381 ccagcgggct atacgtggga aaggagctac ctctttgagg acggagcggt ctgtcgatgc 1441 aacgtggaca taacagtctc tgaaaaggag aactgcatct atcacaaaag tatcttcaga 1501 ggggtgaatt ttcccgccga cggccccgta atgaagaaaa tgaccactaa ttgggaagct 1561 agtaccgaga aaattgtgcc tgttccaaag caagggatat taaagggaaa ggtcaaaatg 1621 tgcctgttgc tgaaggatgg cggtcgttat cattgccagt ttgatacggt atataaagct 1681 aaatcagtgc cctccaagat gccagaatgg cattttatac agcataagct ccttagggag 1741 gaccgctctg atgctaaaaa ccagaaatgg caattaacag aacatgcaat cgcaggcatg 1801 gacgagctgt acaagtaagc ggccgcgact ctagatcata atcagccata ccacatttgt 1861 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat 1921 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa 1981 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 2041 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg 2101 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc 2161 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga 2221 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg 2281 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag 2341 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga 2401 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg 2461 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg 2521 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 2581 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 2641 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg 2701 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 2761 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 2821 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 2881 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 2941 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 3001 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag 3061 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 3121 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 3181 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag 3241 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 3301 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 3361 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 3421 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 3481 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 3541 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg 3601 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 3661 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 3721 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 3781 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 3841 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga 3901 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 3961 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg 4021 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat 4081 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc 4141 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt 4201 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa 4261 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt 4321 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 4381 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 4441 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 4501 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 4561 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg 4621 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 4681 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 4741 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 4801 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 4861 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 4921 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 4981 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 5041 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 5101 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc attagttatt 5161 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cg //