LOCUS Exported 5100 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Nucleus/Histones, Excitation: 530, Emission: 541. ACCESSION . VERSION . KEYWORDS mPapaya1-H2B-6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5100) AUTHORS Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, Davidson MW, Campbell RE TITLE An engineered monomeric Zoanthus sp. yellow fluorescent protein. JOURNAL Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3. PUBMED 24094838 REFERENCE 2 (bases 1 to 5100) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5100 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 597..974 /codon_start=1 /gene="HIST1H2BJ" /product="human histone H2B" /label=H2B /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP GELAKHAVSEGTKAITKYTSAK" regulatory 987..996 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 993..1706 /codon_start=1 /product="monomeric yellow fluorescent protein derived from the Zoanthus fluorescent protein zFP538" /label=mPapaya1 /note="mammalian codon-optimized" /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSYLF EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ GILKGKVKMCLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW QLTEHAIAGMDELYK" polyA_signal 1828..1949 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1865..1884) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1919..1938 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1956..2411) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2093..2114) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2305..2324 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2438..2542 /gene="bla" /label=AmpR promoter promoter 2544..2901 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2567..2587) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2752..2887 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2814..2833 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2936..3730 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2990..3009) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3600..3619 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3918..3937) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3962..4009 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4338..4926 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4827..4846 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 541 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gccaccatgc 601 cagagccagc gaagtctgct cccgccccga aaaagggctc caagaaggcg gtgactaagg 661 cgcagaagaa aggcggcaag aagcgcaagc gcagccgcaa ggagagctat tccatctatg 721 tgtacaaggt tctgaagcag gtccaccctg acaccggcat ttcgtccaag gccatgggca 781 tcatgaattc gtttgtgaac gacattttcg agcgcatcgc aggtgaggct tcccgcctgg 841 cgcattacaa caagcgctcg accatcacct ccagggagat ccagacggcc gtgcgcctgc 901 tgctgcctgg ggagttggcc aagcacgccg tgtccgaggg tactaaggcc atcaccaagt 961 acaccagcgc taaggatcca ccggtcgcca ccatggtgag caagggcgag gggcaatcga 1021 agcatggact aaaggaggag atgacggtga agtaccacat ggaaggttgc gttaatggtc 1081 acaaatttgt cattactgga gagggcattg gaaacccttt taagggtaag caaaccgcaa 1141 atttgtgtgt gatagaagga ggcccgctgc cgttctcgga ggacatttta agtccgggtt 1201 ttaaatatgg tgaccggatt ttcacagagt acccacagga tattgtagat tacttcaaga 1261 actcatgtcc agcgggctat acgtgggaaa ggagctacct ctttgaggac ggagcggtct 1321 gtcgatgcaa cgtggacata acagtctctg aaaaggagaa ctgcatctat cacaaaagta 1381 tcttcagagg ggtgaatttt cccgccgacg gccccgtaat gaagaaaatg accactaatt 1441 gggaagctag taccgagaaa attgtgcctg ttccaaagca agggatatta aagggaaagg 1501 tcaaaatgtg cctgttgctg aaggatggcg gtcgttatca ttgccagttt gatacggtat 1561 ataaagctaa atcagtgccc tccaagatgc cagaatggca ttttatacag cataagctcc 1621 ttagggagga ccgctctgat gctaaaaacc agaaatggca attaacagaa catgcaatcg 1681 caggcatgga cgagctgtac aagtaagcgg ccgcgactct agatcataat cagccatacc 1741 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa 1801 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa 1861 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 1921 ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt 1981 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg 2041 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg 2101 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta 2161 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg 2221 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa 2281 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct 2341 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct 2401 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 2461 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 2521 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag 2581 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 2641 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 2701 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc 2761 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 2821 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 2881 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat 2941 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta 3001 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca 3061 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga 3121 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga 3181 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct 3241 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg 3301 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga 3361 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca 3421 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga 3481 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg 3541 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc 3601 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt 3661 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga 3721 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca 3781 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc 3841 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac 3901 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg 3961 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg 4021 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac 4081 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc 4141 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag 4201 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 4261 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 4321 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 4381 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 4441 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 4501 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 4561 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 4621 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 4681 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 4741 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 4801 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 4861 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 4921 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 4981 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat 5041 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg //