LOCUS       Exported                5772 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Localization: Actin, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    mPapaya-Actin-C-18
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             553..1263
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSFLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMYLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
     polyA_signal    2572..2693
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2609..2628)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2663..2682
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2700..3155)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2837..2858)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3049..3068
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3182..3286
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3311..3331)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3355..3565
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3377..3573
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3424..3559
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3486..3505
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3608..4402
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3662..3681)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4272..4291
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4590..4609)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4634..4681
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5010..5598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5499..5518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gggcaatcga agcatgggct aaaggaggag
      601 atgacggtga agtaccacat ggaaggttgc gttaatggtc acaaatttgt cattactgga
      661 gagggcattg gaaacccttt taagggtaag caaactgcaa atttgtgtgt gatagaagga
      721 ggcccgctgc cgttctcgga ggacatttta agtccgggtt ttaaatatgg tgaccggatt
      781 ttcacagagt acccacagga tattgtagat tacttcaaga actcatgtcc agcgggctat
      841 acgtgggaaa ggagcttcct ctttgaggac ggagcggtct gtcgatgcaa cgtggacata
      901 acagtctctg aaaaggagaa ctgcatctat cacaaaagta tcttcagagg ggtgaatttt
      961 cccgccgacg gccccgtaat gaagaaaatg accactaatt gggaagctag taccgagaaa
     1021 attgtgcctg ttccaaagca agggatatta aaaggaaagg tcaaaatgta cctattgctg
     1081 aaggatggcg gtcgttatca ttgccagttt gatacggtat ataaagctaa atcagtgccc
     1141 tccaagatgc cagaatggca ttttatacag cataagctcc ttagggagga ccgctctgat
     1201 gctaaaaacc agaaatggca attaacagaa catgcaatcg caggcatgga cgagctgtac
     1261 aagtccggac tcagatctgg cagcggtgga ggcagcgcat ccggcggaag cggaagcgat
     1321 gatgatatcg ccgcgctcgt cgtcgacaac ggctccggca tgtgcaaggc cggcttcgcg
     1381 ggcgacgatg ccccccgggc cgtcttcccc tccatcgtgg ggcgccccag gcaccagggc
     1441 gtgatggtgg gcatgggtca gaaggattcc tatgtgggcg acgaggccca gagcaagaga
     1501 ggcatcctca ccctgaagta ccccatcgag cacggcatcg tcaccaactg ggacgacatg
     1561 gagaaaatct ggcaccacac cttctacaat gagctgcgtg tggctcccga ggagcacccc
     1621 gtgctgctga ccgaggcccc cctgaacccc aaggccaacc gcgagaagat gacccagatc
     1681 atgtttgaga ccttcaacac cccagccatg tacgttgcta tccaggctgt gctatccctg
     1741 tacgcctctg gccgtaccac tggcatcgtg atggactccg gtgacggggt cacccacact
     1801 gtgcccatct acgaggggta tgccctcccc catgccatcc tgcgtctgga cctggctggc
     1861 cgggacctga ctgactacct catgaagatc ctcaccgagc gcggctacag cttcaccacc
     1921 acggccgagc gggaaatcgt gcgtgacatt aaggagaagc tgtgctacgt cgccctggac
     1981 ttcgagcaag agatggccac ggctgcttcc agctcctccc tggagaagag ctacgagctg
     2041 cctgacggcc aggtcatcac cattggcaat gagcggttcc gctgccctga ggcactcttc
     2101 cagccttcct tcctgggcat ggagtcctgt ggcatccacg aaactacctt caactccatc
     2161 atgaagtgtg acgtggacat ccgcaaagac ctgtacgcca acacagtgct gtctggcggc
     2221 accaccatgt accctggcat tgccgacagg atgcagaagg agatcactgc cctggcaccc
     2281 agcacaatga agatcaagat cattgctcct cctgagcgca agtactccgt gtggatcggc
     2341 ggctccatcc tggcctcgct gtccaccttc cagcagatgt ggatcagcaa gcaggagtat
     2401 gacgagtccg gcccctccat cgtccaccgc aaatgcttct agggatccac cggatctaga
     2461 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     2521 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     2581 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     2641 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta
     2701 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     2761 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     2821 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     2881 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     2941 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     3001 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     3061 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     3121 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     3181 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3241 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag
     3301 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     3361 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     3421 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3481 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     3541 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     3601 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     3661 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     3721 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     3781 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     3841 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     3901 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     3961 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     4021 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     4081 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     4141 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     4201 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     4261 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     4321 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     4381 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     4441 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     4501 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     4561 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
     4621 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     4681 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     4741 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     4801 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
     4861 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     4921 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4981 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5041 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5101 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     5161 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5221 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5281 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5341 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5401 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5461 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5521 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5581 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5641 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5701 accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     5761 tatggagttc cg
//
