LOCUS Exported 5853 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Mitochondria, Excitation: 530, Emission: 541. ACCESSION . VERSION . KEYWORDS mPapaya-PDHA1-N-10 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5853) AUTHORS Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, Davidson MW, Campbell RE TITLE An engineered monomeric Zoanthus sp. yellow fluorescent protein. JOURNAL Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3. PUBMED 24094838 REFERENCE 2 (bases 1 to 5853) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5853 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 1740..1749 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1746..2459 /codon_start=1 /product="monomeric yellow fluorescent protein derived from the Zoanthus fluorescent protein zFP538" /label=mPapaya1 /note="mammalian codon-optimized" /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSFLF EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ GILKGKVKMYLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW QLTEHAIAGMDELYK" polyA_signal 2581..2702 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2618..2637) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2672..2691 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2709..3164) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2846..2867) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3058..3077 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3191..3295 /gene="bla" /label=AmpR promoter promoter 3297..3654 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3320..3340) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3505..3640 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3567..3586 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3689..4483 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3743..3762) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4353..4372 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4671..4690) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4715..4762 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5091..5679 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5580..5599 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcaccg 541 ccaccatgag gaagatgctc gccgccgtct cccgcgtgct gtctggcgct tctcagaagc 601 cggcaagcag agtgctggta gcatcccgta attttgcaaa tgatgctaca tttgaaatta 661 agaaatgtga ccttcaccgg ctggaagaag gccctcctgt cacaacagtg ctcaccaggg 721 aggatgggct caaatactac aggatgatgc agactgtacg ccgaatggag ttgaaagcag 781 atcagctgta taaacagaaa attattcgtg gtttctgtca cttgtgtgat ggtcaggaag 841 cttgctgtgt gggcctggag gccggcatca accccacaga ccatctcatc acagcctacc 901 gggctcacgg ctttactttc acccggggcc tttccgtccg agaaattctc gcagagctta 961 caggacgaaa aggaggttgt gctaaaggga aaggaggatc gatgcacatg tatgccaaga 1021 acttctacgg gggcaatggc atcgtgggag cgcaggtgcc cctgggcgct gggattgctc 1081 tagcctgtaa gtataatgga aaagatgagg tctgcctgac tttatatggc gatggtgctg 1141 ctaaccaggg ccagatattc gaagcttaca acatggcagc tttgtggaaa ttaccttgta 1201 ttttcatctg tgagaataat cgctatggaa tgggaacgtc tgttgagaga gcggcagcca 1261 gcactgatta ctacaagaga ggcgatttca ttcctgggct gagagtggat ggaatggata 1321 tcctgtgcgt ccgagaggca acaaggtttg ctgctgccta ttgtagatct gggaaggggc 1381 ccatcctgat ggagctgcag acttaccgtt accacggaca cagtatgagt gaccctggag 1441 tcagttaccg tacacgagaa gaaattcagg aagtaagaag taagagtgac cctattatgc 1501 ttctcaagga caggatggtg aacagcaatc ttgccagtgt ggaagaacta aaggaaattg 1561 atgtggaagt gaggaaggag attgaggatg ctgcccagtt tgccacggcc gatcctgagc 1621 cacctttgga agagctgggc taccacatct actccagcga cccacctttt gaagttcgtg 1681 gtgccaatca gtggatcaag tttaagtcag tcagtagctc cggaggggat ccaccggtcg 1741 ccaccatggt gagcaagggc gaggggcaat cgaagcatgg gctaaaggag gagatgacgg 1801 tgaagtacca catggaaggt tgcgttaatg gtcacaaatt tgtcattact ggagagggca 1861 ttggaaaccc ttttaagggt aagcaaactg caaatttgtg tgtgatagaa ggaggcccgc 1921 tgccgttctc ggaggacatt ttaagtccgg gttttaaata tggtgaccgg attttcacag 1981 agtacccaca ggatattgta gattacttca agaactcatg tccagcgggc tatacgtggg 2041 aaaggagctt cctctttgag gacggagcgg tctgtcgatg caacgtggac ataacagtct 2101 ctgaaaagga gaactgcatc tatcacaaaa gtatcttcag aggggtgaat tttcccgccg 2161 acggccccgt aatgaagaaa atgaccacta attgggaagc tagtaccgag aaaattgtgc 2221 ctgttccaaa gcaagggata ttaaaaggaa aggtcaaaat gtacctattg ctgaaggatg 2281 gcggtcgtta tcattgccag tttgatacgg tatataaagc taaatcagtg ccctccaaga 2341 tgccagaatg gcattttata cagcataagc tccttaggga ggaccgctct gatgctaaaa 2401 accagaaatg gcaattaaca gaacatgcaa tcgcaggcat ggacgagctg tacaagtaag 2461 cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta 2521 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt 2581 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 2641 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 2701 taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat 2761 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata 2821 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt 2881 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc 2941 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa 3001 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg 3061 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt 3121 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg 3181 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc 3241 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga 3301 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc 3361 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag 3421 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 3481 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct 3541 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 3601 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga 3661 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc 3721 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 3781 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 3841 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc 3901 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 3961 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 4021 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 4081 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 4141 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 4201 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 4261 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 4321 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 4381 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 4441 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc 4501 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc 4561 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag 4621 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg 4681 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg 4741 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc 4801 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc 4861 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct 4921 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt 4981 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 5041 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 5101 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 5161 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 5221 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct 5281 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 5341 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 5401 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 5461 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 5521 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 5581 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 5641 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 5701 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 5761 gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat caattacggg 5821 gtcattagtt catagcccat atatggagtt ccg //