LOCUS Exported 5163 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expression of ARL3. ACCESSION . VERSION . KEYWORDS CS51 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5163) AUTHORS Cavenagh MM, Breiner M, Schurmann A, Rosenwald AG, Terui T, Zhang C, Randazzo PA, Adams M, Joost HG, Kahn RA TITLE ADP-ribosylation factor (ARF)-like 3, a new member of the ARF family of GTP-binding proteins cloned from human and rat tissues. JOURNAL J Biol Chem. 1994 Jul 22;269(29):18937-42. PUBMED 8034651 REFERENCE 2 (bases 1 to 5163) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5163 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 27..46 /label=T7 /note="T7 promoter, forward primer" promoter 27..45 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" RBS 77..99 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" primer_bind complement(719..737) /label=T7 Term /note="T7 terminator, reverse primer" terminator 733..780 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" promoter complement(1146..1174) /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" primer_bind complement(1201..1219) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 1287..1391 /gene="bla" /label=AmpR promoter CDS 1392..2252 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1610..1629) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2423..3011 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2912..2931 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3165..3182 /label=L4440 /note="L4440 vector, forward primer" misc_feature 3197..3336 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(3262..3281) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3422..3444 /label=pGEX 3' /note="pGEX vectors, reverse primer" CDS complement(3438..3629) /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /label=rop /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" primer_bind 5087..5106 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" ORIGIN 1 ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt 61 tccctctaga aataattttg tttaacttta agaaggagat atacatatgg gcttgctctc 121 aattttgcgc aagttgaaaa gtgcaccaga ccaggaggtg agaatacttc tcctgggctt 181 ggataatgct ggcaagacca ctcttctgaa gcagcttgca tctgaagaca tcagccacat 241 cacacctaca cagggtttca acatcaaaag tgtacaatca caaggtttta aactgaatgt 301 atgggacatt ggtggacaga ggaaaatcag accatactgg aagaattatt ttgaaaatac 361 cgatattctt atatatgtaa tcgacagtgc agacagaaaa agatttgaag agacgggtca 421 ggaactagcg gaattactgg aggaagaaaa actaagttgt gtgccagtgc tcatctttgc 481 taataagcag gatttgctca cagcagcccc tgcctctgaa attgcagaag gactgaacct 541 gcataccatc cgcgaccgag tctggcagat ccagtcttgc tcagctctca caggagaggg 601 cgttcaggat ggcatgaact gggtctgcaa aaacgtcaat gcaaagaaga aataaaatct 661 aggtcgacgg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc 721 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg 781 ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta 841 gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga 901 cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac 961 gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac 1021 cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag 1081 cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact 1141 accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg 1201 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt 1261 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 1321 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 1381 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 1441 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 1501 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 1561 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 1621 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc 1681 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 1741 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 1801 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 1861 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 1921 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 1981 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 2041 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 2101 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 2161 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 2221 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 2281 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 2341 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 2401 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 2461 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 2521 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 2581 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 2641 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 2701 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 2761 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 2821 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 2881 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 2941 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 3001 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 3061 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 3121 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 3181 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac 3241 accgcaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac 3301 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct 3361 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc 3421 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg 3481 cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg 3541 tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg 3601 ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc 3661 atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat 3721 gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg 3781 gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt 3841 ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct 3901 gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct 3961 caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca 4021 ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg 4081 atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg 4141 gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc 4201 cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg 4261 cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga 4321 caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg 4381 cataaatcgc cgtgacgatc agcggtccaa tgatcgaagt taggctggta agagccgcga 4441 gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca 4501 acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc 4561 ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa 4621 tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg 4681 cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc 4741 ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa 4801 agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga 4861 ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta 4921 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat 4981 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga 5041 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga 5101 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt 5161 aga //