LOCUS Exported 9463 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Synaptic transmission. ACCESSION . VERSION . KEYWORDS LZF40: LZF40_Fsyn_Cre SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9463) AUTHORS Adam Y, Kim JJ, Lou S, Zhao Y, Xie ME, Brinks D, Wu H, Mostajo-Radji MA, Kheifets S, Parot V, Chettih S, Williams KJ, Gmeiner B, Farhi SL, Madisen L, Buchanan EK, Kinsella I, Zhou D, Paninski L, Harvey CD, Zeng H, Arlotta P, Campbell RE, Cohen AE TITLE Voltage imaging and optogenetics reveal behaviour-dependent changes in hippocampal dynamics. JOURNAL Nature. 2019 May 1. pii: 10.1038/s41586-019-1166-7. doi: 10.1038/s41586-019-1166-7. PUBMED 31043747 REFERENCE 2 (bases 1 to 9463) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9463 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2632..3079 /label=hSyn promoter /note="human synapsin I promoter; confers neuron-specific expression (Kugler et al., 2003)" CDS 3099..4130 /codon_start=1 /product="site-specific recombinase" /label=Cre /note="Cre is a site-specific recombinase from bacteriophage P1. Recombination occurs at loxP sequences." /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE DGD" misc_feature 4155..4743 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4208..4228) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4626..4637) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(4746..4762) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4747..4763) /label=pBluescriptKS /note="For pBluescript vector" LTR 5268..5448 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(5474..5491) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5480..5704 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 5750..6178 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5837..5856) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6047..6068 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(6187..6207) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 6192..6521 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6372..6507 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6434..6453 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 6569..6616 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 6635..7009 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 7139..7260 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7176..7195) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7230..7249 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(7309..7325) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7309..7325) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7322..7344) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7333..7349 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7357..7387) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7402..7423 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7540..7557) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7711..8299) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7791..7810) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8470..9330) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9093..9112 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9331..9435) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta agtgtctaga ctgcagaggg ccctgcgtat gagtgcaagt 2641 gggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg accccgaccc 2701 actggacaag cacccaaccc ccattcccca aattgcgcat cccctatcag agagggggag 2761 gggaaacagg atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc 2821 ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc 2881 actcgccggt cccccgcaaa ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc 2941 cggcccagcc ggaccgcacc acgcgaggcg cgagataggg gggcacgggc gcgaccatct 3001 gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt 3061 gtcgtgcctg agagcgcagt cgagaaggat ccgccaccat gagcaattta ctgaccgtac 3121 accaaaattt gcctgcatta ccggtcgatg caacgagtga tgaggttcgc aagaacctga 3181 tggacatgtt cagggatcgc caggcgtttt ctgagcatac ctggaaaatg cttctgtccg 3241 tttgccggtc gtgggcggca tggtgcaagt tgaataaccg gaaatggttt cccgcagaac 3301 ctgaagatgt tcgcgattat cttctatatc ttcaggcgcg cggtctggca gtaaaaacta 3361 tccagcaaca tttgggccag ctaaacatgc ttcatcgtcg gtccgggctg ccacgaccaa 3421 gtgacagcaa tgctgtttca ctggttatgc ggcggatccg aaaagaaaac gttgatgccg 3481 gtgaacgtgc aaaacaggct ctagcgttcg aacgcactga tttcgaccag gttcgttcac 3541 tcatggaaaa tagcgatcgc tgccaggata tacgtaatct ggcatttctg gggattgctt 3601 ataacaccct gttacgtata gccgaaattg ccaggatcag ggttaaagat atctcacgta 3661 ctgacggtgg gagaatgtta atccatattg gcagaacgaa aacgctggtt agcaccgcag 3721 gtgtagagaa ggcacttagc ctgggggtaa ctaaactggt cgagcgatgg atttccgtct 3781 ctggtgtagc tgatgatccg aataactacc tgttttgccg ggtcagaaaa aatggtgttg 3841 ccgcgccatc tgccaccagc cagctatcaa ctcgcgccct ggaagggatt tttgaagcaa 3901 ctcatcgatt gatttacggc gctaaggatg actctggtca gagatacctg gcctggtctg 3961 gacacagtgc ccgtgtcgga gccgcgcgag atatggcccg cgctggagtt tcaataccgg 4021 agatcatgca agctggtggc tggaccaatg taaatattgt catgaactat atccgtaacc 4081 tggatagtga aacaggggca atggtgcgcc tgctggaaga tggcgattaa gaattcgata 4141 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 4201 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 4261 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 4321 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 4381 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 4441 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 4501 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct 4561 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 4621 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 4681 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 4741 cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata 4801 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt 4861 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta 4921 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag 4981 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca 5041 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag 5101 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg 5161 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc 5221 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt 5281 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 5341 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 5401 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa 5461 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc 5521 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag 5581 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag 5641 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct 5701 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt 5761 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 5821 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 5881 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 5941 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 6001 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 6061 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 6121 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa 6181 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa 6241 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 6301 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 6361 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 6421 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga 6481 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta 6541 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg 6601 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc 6661 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt 6721 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt 6781 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg 6841 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc 6901 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg 6961 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg 7021 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga 7081 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa 7141 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 7201 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 7261 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt 7321 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 7381 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 7441 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 7501 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 7561 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 7621 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 7681 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 7741 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 7801 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 7861 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 7921 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 7981 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 8041 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 8101 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 8161 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 8221 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 8281 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 8341 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 8401 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 8461 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 8521 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 8581 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 8641 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 8701 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 8761 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 8821 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 8881 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 8941 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 9001 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 9061 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 9121 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 9181 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 9241 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 9301 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 9361 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 9421 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gac //