LOCUS Exported 16038 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells. ACCESSION . VERSION . KEYWORDS pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q109 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 16038) AUTHORS Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith CH TITLE Design and characterization of mutant and wildtype huntingtin proteins produced from a toolkit of scalable eukaryotic expression systems. JOURNAL J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 10.1074/jbc.RA118.007204. Epub 2019 Mar 6. PUBMED 30842263 REFERENCE 2 (bases 1 to 16038) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..16038 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 102..556 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(189..208) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 398..419 /label=F1ori-F /note="F1 origin, forward primer" promoter 582..686 /gene="bla" /label=AmpR promoter CDS 687..1547 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(905..924) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1718..2306 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2207..2226 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2460..2477 /label=L4440 /note="L4440 vector, forward primer" mobile_element 2611..2835 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (right end of the Tn7 transposon)" CDS complement(2902..3435) /codon_start=1 /gene="aacC1" /product="gentamycin acetyltransferase" /label=GmR /note="confers resistance to gentamycin" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" promoter complement(3624..3652) /gene="intI1 (promoter lies within the coding sequence)" /label=Pc promoter /note="class 1 integron promoter" polyA_signal 4160..4208 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" primer_bind 4233..4252 /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" enhancer 4313..4692 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 4693..4896 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 4847..4867 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" promoter 4943..5052 /label=p10 promoter /note="baculovirus promoter for expression in insect cells" regulatory 14091..14100 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 14786..14809 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 14831..14860 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS 14861..14884 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" primer_bind complement(14974..14994) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(15392..15403) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 15628..15762 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(15665..15684) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 15719..15738 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" mobile_element 15791..15956 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (left end of the Tn7 transposon)" ORIGIN 1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga 61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc 121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga 421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata 541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc 2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg 2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa 2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga 2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact 2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg 2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc 2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac 2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc 3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttcaaga gattgatgag 3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat 3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc 3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta 3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag 3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat 3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt 3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg 3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga 3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg 3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc 3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg 3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg 3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc 3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag 3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct 3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg 4021 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa 4081 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg 4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt 4201 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct 4261 cccccatctc ccggtaccgc atgcggatct gtacgggcca gatatacgcg ttgacattga 4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg 4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 4681 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 4741 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 4801 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 4861 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact 4921 gcttactggc ttatcgaata cggaccttta attcaaccca acacaatata ttatagttaa 4981 ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt 5041 ttatttacaa tcactcgacg gatccttaag aaggagatat actatggcga ccctggaaaa 5101 gctgatgaag gccttcgagt ccctcaagtc cttccaacag cagcaacagc aacaacagca 5161 gcaacagcaa caacagcagc aacagcaaca acagcagcaa cagcaacaac agcagcaaca 5221 gcaacaacag cagcaacagc aacaacaaca gcagcaacag caacaacagc agcaacagca 5281 acaacagcag caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca 5341 gcagcaacag caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca 5401 acagcaacaa cagcagcaac agcaacaaca gcagcaacag caacaacagc agcaacagca 5461 accgccaccg ccgccgccgc cgccgccgcc tcctcagctt cctcagccgc cgccgcaggc 5521 acagccgctg ctgcctcagc cgcagccgcc cccgccgccg cccccgccgc cacccggccc 5581 ggctgtggct gaggagccgc tgcaccgacc aaagaaagaa ctttcagcta ccaagaaaga 5641 ccgtgtgaat cattgtctga caatatgtga aaacatagtg gcacagtctg tcagaaattc 5701 tccagaattt cagaaacttc tgggcatcgc tatggaactt tttctgctgt gcagtgatga 5761 cgcagagtca gatgtcagga tggtggctga cgaatgcctc aacaaagtta tcaaagcttt 5821 gatggattct aatcttccaa ggttacagct cgagctctat aaggaaatta aaaagaatgg 5881 tgcccctcgg agtttgcgtg ctgccctgtg gaggtttgct gagctggctc acctggttcg 5941 gcctcagaaa tgcaggcctt acctggtgaa ccttctgccg tgcctgactc gaacaagcaa 6001 gagacccgaa gaatcagtcc aggagacctt ggctgcagct gttcccaaaa ttatggcttc 6061 ttttggcaat tttgcaaatg acaatgaaat taaggttttg ttaaaggcct tcatagcgaa 6121 cctgaagtca agctccccca ccattcggcg gacagcggct ggatcagcag tgagcatctg 6181 ccagcactca agaaggacac aatatttcta tagttggcta ctaaatgtgc tcttaggctt 6241 actcgttcct gtcgaggatg aacactccac tctgctgatt cttggcgtgc tgctcaccct 6301 gaggtatttg gtgcccttgc tgcagcagca ggtcaaggac acaagcctga aaggcagctt 6361 cggagtgaca aggaaagaaa tggaagtctc tccttctgca gagcagcttg tccaggttta 6421 tgaactgacg ttacatcata cacagcacca agaccacaat gttgtgaccg gagccctgga 6481 gctgttgcag cagctcttca gaacgcctcc acccgagctt ctgcaaaccc tgaccgcagt 6541 cgggggcatt gggcagctca ccgctgctaa ggaggagtct ggtggccgaa gccgtagtgg 6601 gagtattgtg gaacttatag ctggaggggg ttcctcatgc agccctgtcc tttcaagaaa 6661 acaaaaaggc aaagtgctct taggagaaga agaagccttg gaggatgact ctgaatcgag 6721 atcggatgtc agcagctctg ccttaacagc ctcagtgaag gatgagatca gtggagagct 6781 ggctgcttct tcaggggttt ccactccagg gtcagcaggt catgacatca tcacagaaca 6841 gccacggtca cagcacacac tgcaggcgga ctcagtggat ctggccagct gtgacttgac 6901 aagctctgcc actgatgggg atgaggagga tatcttgagc cacagctcca gccaggtcag 6961 cgccgtccca tctgaccctg ccatggacct gaatgatggg acccaggcct cgtcgcccat 7021 cagcgacagc tcccagacca ccaccgaagg gcctgattca gctgttaccc cttcagacag 7081 ttctgaaatt gtgttagacg gtaccgacaa ccagtatttg ggcctgcaga ttggacagcc 7141 ccaggatgaa gatgaggaag ccacaggtat tcttcctgat gaagcctcgg aggccttcag 7201 gaactcttcc atggcccttc aacaggcaca tttattgaaa aacatgagtc actgcaggca 7261 gccttctgac agcagtgttg ataaatttgt gttgagagat gaagctactg aaccgggtga 7321 tcaagaaaac aagccttgcc gcatcaaagg tgacattgga cagtccactg atgatgactc 7381 tgcacctctt gtccattgtg tccgcctttt atctgcttcg tttttgctaa cagggggaaa 7441 aaatgtgctg gttccggaca gggatgtgag ggtcagcgtg aaggccctgg ccctcagctg 7501 tgtgggagca gctgtggccc tccacccgga atctttcttc agcaaactct ataaagttcc 7561 tcttgacacc acggaatacc ctgaggaaca gtatgtctca gacatcttga actacatcga 7621 tcatggagac ccacaggttc gaggagccac tgccattctc tgtgggaccc tcatctgctc 7681 catcctcagc aggtcccgct tccacgtggg agattggatg ggcaccatta gaaccctcac 7741 aggaaataca ttttctttgg cggattgcat tcctttgctg cggaaaacac tgaaggatga 7801 gtcttctgtt acttgcaagt tagcttgtac agctgtgagg aactgtgtca tgagtctctg 7861 cagcagcagc tacagtgagt taggactgca gctgatcatc gatgtgctga ctctgaggaa 7921 cagttcctat tggctggtga ggacagagct tctggaaacc cttgcagaga ttgacttcag 7981 gctggtgagc tttttggagg caaaagcaga aaacttacac agaggggctc atcattatac 8041 agggctttta aaactgcaag aacgagtgct caataatgtt gtcatccatt tgcttggaga 8101 tgaagacccc agggtgcgac atgttgccgc agcatcacta attaggcttg tcccaaagct 8161 gttttataaa tgtgaccaag gacaagctga tccagtagtg gccgtggcaa gagatcaaag 8221 cagtgtttac ctgaaacttc tcatgcatga gacgcagcct ccatctcatt tctccgtcag 8281 cacaataacc agaatatata gaggctataa cctactacca agcataacag acgtcactat 8341 ggaaaataac ctttcaagag ttattgcagc agtttctcat gaactaatca catcaaccac 8401 cagagcactc acatttggat gctgtgaagc tttgtgtctt ctttccactg ccttcccagt 8461 ttgcatttgg agtttaggtt ggcactgtgg agtgcctcca ctgagtgcct cagatgagtc 8521 taggaagagc tgtaccgttg ggatggccac aatgattctg accctgctct cgtcagcttg 8581 gttcccattg gatctctcag cccatcaaga tgctttgatt ttggccggaa acttgcttgc 8641 agccagtgct cccaaatctc tgagaagttc atgggcctct gaagaagaag ccaacccagc 8701 agccaccaag caagaggagg tctggccagc cctgggggac cgggccctgg tgcccatggt 8761 ggagcagctc ttctctcacc tgctgaaggt gattaacatt tgtgcccacg tcctggatga 8821 cgtggctcct ggacccgcaa taaaggcagc cttgccttct ctaacaaacc ccccttctct 8881 aagtcccatc cgacgaaagg ggaaggagaa agaaccagga gaacaagcat ctgtaccgtt 8941 gagtcccaag aaaggcagtg aggccagtgc agcttctaga caatctgata cctcaggtcc 9001 tgttacaaca agtaaatcct catcactggg gagtttctat catcttcctt catacctcaa 9061 actgcatgat gtcctgaaag ctacacacgc taactacaag gtcacgctgg atcttcagaa 9121 cagcacggaa aagtttggag ggtttcttcg ctcagccttg gatgttcttt ctcagatact 9181 agagctggcc acactgcagg acattgggaa gtgtgttgaa gagatcctag gatacctgaa 9241 atcctgcttt agtcgagaac caatgatggc aactgtttgt gttcaacaat tgttgaagac 9301 tctctttggc acaaacttgg cctcccagtt tgatggctta tcttccaacc ccagcaagtc 9361 acaaggccga gcacagcgcc ttggctcctc cagtgtgagg ccaggcttgt accactactg 9421 cttcatggcc ccgtacaccc acttcaccca ggccctcgct gacgccagcc tgaggaacat 9481 ggtgcaggcg gagcaggaga acgacacctc gggatggttt gatgtcctcc agaaagtgtc 9541 tacccagttg aagacaaacc tcacgagtgt cacaaagaac cgtgcagata agaatgctat 9601 tcataatcac attcgtttgt ttgaacctct tgttataaaa gctttaaaac agtacacgac 9661 tacaacatgt gtgcagttac agaagcaggt tttagatttg ctggcgcagc tggttcagtt 9721 acgggttaat tactgtcttc tggattcaga tcaggtgttt attggctttg tattgaaaca 9781 gtttgaatac attgaagtgg gccagttcag ggaatcagag gcaatcattc caaacatctt 9841 tttcttcttg gtattactat cttatgaacg ctatcattca aaacagatca ttggaattcc 9901 taaaatcatt cagctctgtg atggcatcat ggccagtgga aggaaggctg tgacacatgc 9961 cataccggct ctgcagccca tagtccacga cctctttgta ttaagaggaa caaataaagc 10021 tgatgcagga aaagagcttg aaacccaaaa agaggtggtg gtgtcaatgt tactgagact 10081 catccagtac catcaggtgt tggagatgtt cattcttgtc ctgcagcagt gccacaagga 10141 gaatgaagac aagtggaagc gactgtctcg acagatagct gacatcatcc tcccaatgtt 10201 agccaaacag cagatgcaca ttgactctca tgaagccctt ggagtgttaa atacattatt 10261 tgagattttg gccccttcct ccctccgtcc ggtagacatg cttttacgga gtatgttcgt 10321 cactccaaac acaatggcgt ccgtgagcac tgttcaactg tggatatcgg gaattctggc 10381 cattttgagg gttctgattt cccagtcaac tgaagatatt gttctttctc gtattcagga 10441 gctctccttc tctccgtatt taatctcctg tacagtaatt aataggttaa gagatgggga 10501 cagtacttca acgctagaag aacacagtga agggaaacaa ataaagaatt tgccagaaga 10561 aacattttca aggtttctat tacaactggt tggtattctt ttagaagaca ttgttacaaa 10621 acagctgaag gtggaaatga gtgagcagca acatactttc tattgccagg aactaggcac 10681 actgctaatg tgtctgatcc acatcttcaa gtctggaatg ttccggagaa tcacagcagc 10741 tgccactagg ctgttccgca gtgatggctg tggcggcagt ttctacaccc tggacagctt 10801 gaacttgcgg gctcgttcca tgatcaccac ccacccggcc ctggtgctgc tctggtgtca 10861 gatactgctg cttgtcaacc acaccgacta ccgctggtgg gcagaagtgc agcagacccc 10921 gaaaagacac agtctgtcca gcacaaagtt acttagtccc cagatgtctg gagaagagga 10981 ggattctgac ttggcagcca aacttggaat gtgcaataga gaaatagtac gaagaggggc 11041 tctcattctc ttctgtgatt atgtctgtca gaacctccat gactccgagc acttaacgtg 11101 gctcattgta aatcacattc aagatctgat cagcctttcc cacgagcctc cagtacagga 11161 cttcatcagt gccgttcatc ggaactctgc tgccagcggc ctgttcatcc aggcaattca 11221 gtctcgttgt gaaaaccttt caactccaac catgctgaag aaaactcttc agtgcttgga 11281 ggggatccat ctcagccagt cgggagctgt gctcacgctg tatgtggaca ggcttctgtg 11341 cacccctttc cgtgtgctgg ctcgcatggt cgacatcctt gcttgtcgcc gggtagaaat 11401 gcttctggct gcaaatttac agagcagcat ggcccagttg ccaatggaag aactcaacag 11461 aatccaggaa taccttcaga gcagcgggct cgctcagaga caccaaaggc tctattccct 11521 gctggacagg tttcgtctct ccaccatgca agactcactt agtccctctc ctccagtctc 11581 ttcccacccg ctggacgggg atgggcacgt gtcactggaa acagtgagtc cggacaaaga 11641 ctggtacgtt catcttgtca aatcccagtg ttggaccagg tcagattctg cactgctgga 11701 aggtgcagag ctggtgaatc ggattcctgc tgaagatatg aatgccttca tgatgaactc 11761 ggagttcaac ctaagcctgc tagctccatg cttaagccta gggatgagtg aaatttctgg 11821 tggccagaag agtgcccttt ttgaagcagc ccgtgaggtg actctggccc gtgtgagcgg 11881 caccgtgcag cagctccctg ctgtccatca tgtcttccag cccgagctgc ctgcagaacc 11941 ggcggcctac tggagcaagt tgaatgatct gtttggggat gctgcactgt atcagtccct 12001 gcccactctg gcccgggccc tggcacagta cctggtggtg gtctccaaac tgcccagtca 12061 tttgcacctt cctcctgaga aagagaagga cattgtgaaa ttcgtggtgg caacccttga 12121 ggccctgtcc tggcatttga tccatgagca gatcccgctg agtctggatc tccaggcagg 12181 gctggactgc tgctgcctgg ccctgcagct gcctggcctc tggagcgtgg tctcctccac 12241 agagtttgtg acccacgcct gctccctcat ccactgtgtg cacttcatcc tggaggccgt 12301 tgcagtgcag cctggagagc agcttcttag tccagaaaga aggacaaata ccccaaaagc 12361 catcagcgag gaggaggagg aagtagatcc aaacacacag aatcctaagt atatcactgc 12421 agcctgtgag atggtggcag aaatggtgga gtctctgcag tcggtgttgg ccttgggtca 12481 taaaaggaat agcggcgtgc cggcgtttct cacgccattg ctcaggaaca tcatcatcag 12541 cctggcccgc ctgccccttg tcaacagcta cacacgtgtg cccccactgg tgtggaagct 12601 tggatggtca cccaaaccgg gaggggattt tggcacagca ttccctgaga tccccgtgga 12661 gttcctccag gaaaaggaag tctttaagga gttcatctac cgcatcaaca cactaggctg 12721 gaccagtcgt actcagtttg aagaaacttg ggccaccctc cttggtgtcc tggtgacgca 12781 gcccctcgtg atggagcagg aggagagccc accagaagaa gacacagaga ggacccagat 12841 caacgtcctg gccgtgcagg ccatcacctc actggtgctc agtgcaatga ctgtgcctgt 12901 ggccggcaac ccagctgtaa gctgcttgga gcagcagccc cggaacaagc ctctgaaagc 12961 tctcgacacc aggtttggga ggaagctgag cattatcaga gggattgtgg agcaagagat 13021 tcaagcaatg gtttcaaaga gagagaatat tgccacccat catttatatc aggcatggga 13081 tcctgtccct tctctgtctc cggctactac aggtgccctc atcagccacg agaagctgct 13141 gctgcagatc aaccccgagc gggagctggg gagcatgagc tacaaactcg gccaggtgtc 13201 catacactcc gtgtggctag ggaacagcat cacacccctg agggaggagg aatgggacga 13261 ggaagaggag gaggaggccg acgcccctgc accttcgtca ccacccacgt ctccagtcaa 13321 ctccaggaaa caccgggctg gagttgacat ccactcctgt tcgcagtttt tgcttgagtt 13381 gtacagccgc tggatcctgc cgtccagctc agccaggagg accccggcca tcctgatcag 13441 tgaggtggtc agatcccttc tagtggtctc agacttgttc accgagcgca accagtttga 13501 gctgatgtat gtgacgctga cagaactgcg aagggtgcac ccttcagaag acgagatcct 13561 cgctcagtac ctggtgcctg ccacctgcaa ggcagctgcc gtccttggga tggacaaggc 13621 cgtggcggag cctgtcagcc gcctgctgga gagcacgctc aggagcagcc acctgcccag 13681 cagggttgga gccctgcacg gcatcctcta tgtgctggag tgcgacctgc tggacgacac 13741 tgccaagcag ctcatcccgg tcatcagcga ctatctcctc tccaacctga aagggatcgc 13801 ccactgcgtg aacattcaca gccagcagca cgtactggtc atgtgtgcca ctgcgtttta 13861 cctcattgag aactatcctc tggacgtagg gccggaattt tcagcatcaa taatacagat 13921 gtgtggggtg atgctgtctg gaagtgagga gtccaccccc tccatcattt accactgtgc 13981 cctcagaggc ctggagcgcc tcctgctctc tgagcagctc tcccgcctgg atgcagaatc 14041 gctggtcaag ctgagtgtgg acagagtgaa cgtgcacagc ccgcaccggg ccatggcggc 14101 tctgggcctg atgctcacct gcatgtacac aggaaaggag aaagtcagtc cgggtagaac 14161 ttcagaccct aatcctgcag cccccgacag cgagtcagtg attgttgcta tggagcgggt 14221 atctgttctt tttgatagga tcaggaaagg ctttccttgt gaagccagag tggtggccag 14281 gatcctgccc cagtttctag acgacttctt cccaccccag gacatcatga acaaagtcat 14341 cggagagttt ctgtccaacc agcagccata cccccagttc atggccaccg tggtgtataa 14401 ggtgtttcag actctgcaca gcaccgggca gtcgtccatg gtccgggact gggtcatgct 14461 gtccctctcc aacttcacgc agagggcccc ggtcgccatg gccacgtgga gcctctcctg 14521 cttctttgtc agcgcgtcca ccagcccgtg ggtcgcggcg atcctcccac atgtcatcag 14581 caggatgggc aagctggagc aggtggacgt gaaccttttc tgcctggtcg ccacagactt 14641 ctacagacac cagatagagg aggagctcga ccgcagggcc ttccagtctg tgcttgaggt 14701 ggttgcagcc ccaggaagcc catatcaccg gctgctgact tgtttacgaa atgtccacaa 14761 ggtcaccacc tgcggtggaa gcggtgacta caaagacgat gacgacaagt aaaacctcta 14821 cttccaatcg caccatcatc accatcacca tcaccaccat gattacaagg atgacgacga 14881 taagtgaggc ggccgccaac ttgatatcaa gcttatcgat aatcaacctc tggattacaa 14941 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 15001 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 15061 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 15121 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 15181 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 15241 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 15301 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat 15361 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 15421 ccgcgggcgc ccccctcctc acggcgagcg aagcttgtcg agaagtacta gaggatctct 15481 agagcctgca gtctcgacaa gcttgtcgag aagtactaga ggatcataat cagccatacc 15541 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa 15601 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa 15661 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 15721 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact gcttgagcct 15781 aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt 15841 cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa 15901 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg 15961 ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc 16021 gtcatcttcg gctacttt //